Total antioxidant capacity t aoc kit

Tartary buckwheat seeds (Hefeng No. 1) were cultivated in Datong, Shanxi Province, China, and were acquired from Beijing Green Valley Sprout Co., Ltd. (Beijing, China). Rutin, gallic acid, and γ-Amino butyric acid standard were purchased from Shanghai solarbio Bioscience & Technology Co., Ltd. (Shanghai, China). The analytical grade chemicals, such as Folin-Ciocalteu reagent, gallic acid (GA), sodium carbonate, sodium hypochlorite, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, phosphoric acid, glucose anhydrous, aluminum chloride, potassium acetate, o-phthalaldehyde, copper sulfate, potassium sulfate, sulfuric acid, boric acid, ethanol, ethyl acetate, hydrochloric acid, trichloroacetic acid, sodium acetate, phenol, potassium persulfate, sodium hydroxide, and aluminum chloride were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). In addition, 2,2-diphenyl picryl hydrazyl (DPPH), acetone, and methanol (HPLC grade) were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Total antioxidant capacity (T-AOC) kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China).

The animal experiment was approved by the Experimental Animal Care Ethics Committee of Northwest A&F University. A total of 320 ICR mice (male, 9 weeks old) with an average weight of 33 ± 2 g, SPF grade, were purchased from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) (Approval Number: SCXK (Beijing) 2012-0001). Before the experiment, all experimental animals were bred adaptively in the animal room for one week. The conditions in the animal room were controlled as follows: the humidity was set to 55% ± 5% and the temperature was set to 25 ± 2 ℃, a 12-h circadian rhythm was ensured, and the experimental animals could drink water and eat freely. After all the mice had adapted to the environment, the experiment was started.
The aspartate aminotransferase (AST) kit, the alanine aminotransferase (ALT) kit, the total bilirubin (TBIL) kit, the alkaline phosphatase (ALP) kit, the total triglyceride (TC) kit, the total cholesterol (TG) kit, the total antioxidant capacity (T-AOC) kit, the catalase (CAT) kit, the glutathione (GSH) kit, the superoxide dismutase (SOD) kit, and the malonaldehyde (MDA) kit were purchased from Nanjing Jiancheng Bioengineering Institute (China).

L. plantarum YW11 was maintained as frozen (−80 °C) stocks in SDM medium supplemented with 20% (v/v) glycerol and adjusted to pH 6.6 with 1 M acetic acid [18 (link),19 (link)]. L. plantarum YW11 was revitalized in SDM medium by two overnight sub-culturing events at 37 °C before use for preparation of EPS.
CAS was purchased from Beijing Mreda Technology Co. Ltd. (Beijing, China), and D-(+)-gluconic acid δ-lactone (GDL) from Beijing Hua Wei Rui Ke Chemical Co. Ltd. (Beijing, China), chenodeoxycholic acid (CDCA) from Sigma–Aldrich (St. Louis, MO, USA), total bile acids (TBA) kit and total antioxidant capacity (T-AOC) kit from Nanjing Jiancheng Bioengineering Institute. Potassium dihydrogen phosphate, potassium citrate, potassium sulfate and calcium chloride were purchased from Beijing Pinellia Technology Development Co., Ltd. (Beijing, China). Sodium hydroxide, sodium citrate, and potassium chloride were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Sodium dihydrogen phosphate and disodium hydrogen phosphate were purchased from Sinopharm Group Chemical Reagent Co. Ltd. (Beijing, China). All reagents were of analytical grade.