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Steponeplus taqman system

Manufactured by Thermo Fisher Scientific
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The StepOnePlus Taqman system is a real-time PCR instrument designed for gene expression analysis, genotyping, and other molecular biology applications. The system utilizes TaqMan technology to detect and quantify target DNA sequences in a sample.

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Lab products found in correlation

Multiscribe, by Thermo Fisher Scientific (2 mentions)

2 protocols using steponeplus taqman system

1

Reverse Transcription and RT-qPCR Analysis

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Reverse transcription was performed using random hexamers and MultiScribe reverse transcriptase (Applied Biosystems). RT‐qPCR analyses were performed using Assay‐on‐Demand primers and probe sets (Applied Biosystems) with a StepOnePlus Taqman system.
Cano M., Datta S., Wang L., Liu T., Flores‐Bellver M., Sachdeva M., Sinha D, & Handa J.T. (2021). Nrf2 deficiency decreases NADPH from impaired IDH shuttle and pentose phosphate pathway in retinal pigmented epithelial cells to magnify oxidative stress‐induced mitochondrial dysfunction. Aging Cell, 20(8), e13444.
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2

Reverse Transcription and RT-qPCR Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Reverse transcription was performed using random hexamers and MultiScribe reverse transcriptase according to the manufacturer’s recommendations (Applied Biosystems, Foster City, California). RT-qPCR analyses were performed using Assay-on-Demand primers and probe sets (Applied Biosystems) with a StepOnePlus Taqman system (Applied Biosystems). β-actin was used for normalization.
Cano M., Wang L., Wan J., Barnett B.P., Ebrahimi K., Qian J, & Handa J.T. (2014). Oxidative Stress Induces Mitochondrial Dysfunction and a Protective Unfolded Protein Response in RPE cells. Free radical biology & medicine, 69, 1-14.
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