Anti β tubulin

Manufactured by GeneTex

Sourced in United States

Anti-β-tubulin is a monoclonal antibody used to detect and quantify the β-tubulin protein, a key component of the cytoskeleton in eukaryotic cells. This antibody can be used in various laboratory techniques, such as Western blotting, immunocytochemistry, and flow cytometry, to study the expression and localization of β-tubulin in biological samples.

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Lab products found in correlation

Anti 4e bp1, by Cell Signaling Technology (1 mentions) Anti nrf2, by Cell Signaling Technology (1 mentions) Anti p53, by Cell Signaling Technology (1 mentions) Anti nqo1, by Santa Cruz Biotechnology (1 mentions) Anti ephrinb2, by Abcam (1 mentions) Anti ve cadherin, by Santa Cruz Biotechnology (1 mentions) Anti notch1 icd, by Abcam (1 mentions) Anti hsp90, by BD (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Anti icam 1 antibody, by R&D Systems (1 mentions) Anti flag, by Merck Group (1 mentions) Anti hsp90, by GeneTex (1 mentions) Anti vcp, by GeneTex (1 mentions) Anti calnexin, by GeneTex (1 mentions) Protease inhibitor, by Merck Group (1 mentions) Phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions) Horseradish peroxidase coupled secondary antibody, by Cell Signaling Technology (1 mentions) Anti p jnk, by Cell Signaling Technology (1 mentions) Anti ubiquitin, by Agilent Technologies (1 mentions) Anti jnk, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

β-tubulin (13 citations) Anti-tubulin (9 citations)

3 protocols using anti β tubulin

Protein Interaction Analysis by Co-IP

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Preparation of cell extracts, co-immunoprecipitation and immunoblotting were carried out as previously described (Pyronnet et al., 1999 (link)). The antibodies used were as follows: anti-eIF4GI and anti-eIF4GII (gifts of Prof. Nahum Sonenberg); anti-DAP5 (CliniSciences #610742); anti-HA-7 (Sigma); anti-β-tubulin (GeneTex #6288022); anti-4E-BP1, anti-NRF2 and anti-p53 (Cell Signaling Technologies #9452, #12721, and #1C12, respectively); anti-Core 20S (Enzo Life Sciences #PW8155); and anti-NQO1 (Santa Cruz #C19).

Alard A., Marboeuf C., Fabre B., Jean C., Martineau Y., Lopez F., Vende P., Poncet D., Schneider R.J., Bousquet C, & Pyronnet S. (2019). Differential Regulation of the Three Eukaryotic mRNA Translation Initiation Factor (eIF) 4Gs by the Proteasome. Frontiers in Genetics, 10, 254.

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Protein Expression Analysis by Western Blot

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Approximately 1 million cells were lysed, and 30 mg of protein was loaded on a 4% to 12% sodium dodecyl sulfate polyacrylamide electrophoresis gel (SDS-PAGE) (Bio-Rad Laboratories) for Western blot analysis. Anti-ICAM-1 antibody (R&D Systems BBA17, Minneapolis, MN, USA) 1:1,000, anti-CD31 (Abcam 28364) 1:400, anti-VE-cadherin 1:100 (Santa Cruz Biotechnology SC6458), anti-β-catenin 1:100 (eBioscience Inc. 14-2567, Santa Clara, CA, USA), anti-Notch1-ICD 1:400 (Abcam 8925), anti-EphrinB2 1:1,000 (Abcam ab96264), anti-β-tubulin 1:4,000 (Genetex GTX628802, Irvine, CA, USA), and anti-HSP90 1:1,000 (BD Biosciences 610419) were used to probe for proteins of interest overnight at 4 °C. Blots were washed and incubated with 1:5,000 Alexa Flour 680 rabbit anti-goat, goat anti-mouse, or donkey anti-rabbit secondary antibodies for 1 h. Protein bands were visualized using the Odyssey Infrared Imaging System (LI-COR Biosciences, Lincoln, NE, USA. Densitometry analysis of the gels was carried out using ImageJ software from the National Institutes of Health (NIH, Bethesda, MD, USA; http://rsbweb.nih.gov/ij/).

Sivarapatna A., Ghaedi M., Xiao Y., Han E., Aryal B., Zhou J., Fernandez-Hernando C., Qyang Y., Hirschi K.K, & Niklason L.E. (2017). Engineered Microvasculature in PDMS Networks Using Endothelial Cells Derived from Human Induced Pluripotent Stem Cells. Cell Transplantation, 26(8), 1365-1379.

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Protein Analysis of Mouse Retinas

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Mouse retinas were lysed in 300 μl lysis buffer (PBS, 0.5 g/ml n-dodecyl-b-D-maltoside (Calbiochem EMD Bioscience), protease inhibitors (Sigma-Aldrich), and phosphatase inhibitor (Thermo Scientific, Rockford, IL)). MEF cells and HEK293 cells with indicated drug treatments were lysed in SDS lysis buffer (2% SDS, 62.5 mM Tris-HCl pH 6.8, containing protease inhibitors and phosphatase inhibitor). The following antibodies and dilutions were used: anti-Gαt1 and 1D4 anti-rod opsin 1:1000 dilution (Santa Cruz Biotechnologies); B630N anti-rhodopsin 1:1000 (gift of W.C. Smith, Gainesville, FL); anti-calnexin 1:1000, anti-VCP at 1:1000, anti-HSP90 at 1:5000, and anti-β-tubulin at 1:5000 (GeneTex); anti-P-JNK at 1:1000 and anti-JNK at 1:1000 (Cell Signaling); anti-flag at 1:5000 (Sigma-Aldrich); and anti-ubiquitin at 1:1000 (Dako). After overnight incubation with primary antibody, membranes were washed followed by incubation with a horseradish peroxidase-coupled secondary antibody (Cell signaling). Immunoreactivity was detected with the SuperSignal West chemiluminescent substrate (Pierce).

Chiang W.C., Kroeger H., Sakami S., Messah C., Yasumura D., Matthes M.T., Coppinger J.A., Palczewski K., LaVail M.M, & Lin J.H. (2014). Robust Endoplasmic Reticulum-Associated Degradation of Rhodopsin Precedes Retinal Degeneration. Molecular neurobiology, 52(1), 679-695.

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