Axio imager m2

Manufactured by Zeiss

Sourced in Germany, United States, Japan, Canada, Spain, Australia, United Kingdom

The Axio Imager M2 is a high-performance microscope system designed for a wide range of applications. It features a stable, vibration-free stand, a versatile illumination system, and advanced optics to deliver high-quality images. The Axio Imager M2 is capable of supporting various imaging techniques, including brightfield, darkfield, and fluorescence microscopy.

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Lab products found in correlation

Lsm 710, by Zeiss (33 mentions) Axiocam mrm, by Zeiss (30 mentions) Axiocam mrc, by Zeiss (30 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (28 mentions) Apotome 2, by Zeiss (25 mentions) Zen software, by Zeiss (23 mentions) Lsm 880, by Zeiss (20 mentions) Apotome, by Zeiss (18 mentions) Axio observer z1, by Zeiss (18 mentions) Axiocam mrm camera, by Zeiss (17 mentions) Axiocam 506, by Zeiss (16 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (14 mentions) Apotome system, by Zeiss (13 mentions) Hoechst 33342, by Thermo Fisher Scientific (12 mentions) Lsm 800, by Zeiss (11 mentions) Zen 2, by Zeiss (11 mentions) Tcs sp8, by Leica camera (10 mentions) Lsm 700, by Zeiss (10 mentions) Axio zoom v16, by Zeiss (10 mentions) Efficient navigation zen , by Zeiss (9 mentions)

1 035 protocols using axio imager m2

Protoplast Isolation and ROS Detection

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Protoplasts were prepared from protonemata as described previously (Fesenko et al., 2015 (link)) and incubated for 48 hours at solid BCD agar medium. Regenerated protoplasts were stained with 10 µg/ml Calcofluor White (Fluorescent Brightener 28) for 5 minutes. After that, protoplasts were analyzed using fluorescent microscope (Axio Imager M2, Zeiss, Germany) at λex = 365 nm, BS FT 395, and λem = 445nm/50 nm (Filter set 49 DAPI, Zeiss, Germany).
The fluorescent dye 2,7-Dichlorofluorescin Diacetate (DCFH-DA, Sigma-Aldrich, USA) was used to identify intracellular ROS. Using a spatula, seven-day-old protonema filaments were removed from the agar surface and transferred to mQ. Protonemata were then treated with 0.0025% driselase (diluted in mQ) for 1 minute or mQ water as a control and incubated with 10 µM DCFH-DA for 15 minutes in total. The No. 44 filter (λex BP 475 nm/40 nm; λem BP 530 nm/50 nm) was used for DCFH-DA fluorescence detection on the fluorescent microscope Axio Imager M2 (Zeiss) with an AxioCam 506 mono digital camera. Data on the fluorescence intensity were obtained from the related Zeiss software Zen.

Mamaeva A., Lyapina I., Knyazev A., Golub N., Mollaev T., Chudinova E., Elansky S., Babenko V.V., Veselovsky V.A., Klimina K.M., Gribova T., Kharlampieva D., Lazarev V, & Fesenko I. (2023). RALF peptides modulate immune response in the moss Physcomitrium patens. Frontiers in Plant Science, 14, 1077301.

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Quantifying Arcade Cell Junctions in C. elegans

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Embryos were placed on a 4% agar pad (Difco Noble Agar) in 10 mM levamisole. A #1.5 coverslip (Corning) was added, and nail polish was added at the corners to prevent the coverslip from moving. Embryos were scored using Nomarski optics for foregut attachment defects under a Zeiss AxioImager M2 running AxioVision or ZEN2 software. For live imaging of GFP fluorescence, embryos were imaged under either an AxioImager M2 with Apotome or Apotome.2 to remove out-of-focus light or the Zeiss LSM710, LSM780, or LSM880 confocal microscope. For AJM-1::GFP quantification in SU93 and SM1271 animals, z-stacks through the foregut/arcade cells were taken using the same gain and laser power for each embryo. The z-stacks were rotated in Zen Blue software (Zeiss) and culled of any noninformative slices. These modified stacks were imported into Fiji software (Schindelin et al., 2012 (link)). The region of interest containing the arcade cells was cropped out of original image and changed to 16 bit, and the stack was z-projected using the Sum Slices setting and then measured to obtain the mean gray value and area. To control for differences in area between samples, the average intensity per pixel was calculated by dividing the mean gray value by the area.

Von Stetina S.E., Liang J., Marnellos G, & Mango S.E. (2017). Temporal regulation of epithelium formation mediated by FoxA, MKLP1, MgcRacGAP, and PAR-6. Molecular Biology of the Cell, 28(15), 2042-2065.

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Brain-Wide Connectivity Analysis Protocol

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For brain-wide connectivity analysis, brain sections were kept in serial order throughout their processing. Sections with one or more GFP (or mCherry)–labeled cell somas were scanned using an epifluorescence microscope with a motorized stage (Zeiss, Axio Imager M2) equipped with a 10× objective [numerical aperture (NA) 0.3]. Automated scanning, tile alignment, and image stitching were performed to create a high-resolution image of the whole section. In sections with unclear cell numbers due to close apposition of two GFP (or mCherry) cell bodies or with high densities of GFP (or mCherry) cells, scanning of Z-stacks in a laser scanning confocal microscope (Zeiss, LSM 710) with a 40× objective (NA 1.1) was carried out. For all immunofluorescence studies, images were acquired using an epifluorescence microscope with a motorized stage (Zeiss, Axio Imager M2) and a laser scanning confocal microscope (Zeiss, LSM 710).

Grade S., Thomas J., Zarb Y., Thorwirth M., Conzelmann K.K., Hauck S.M, & Götz M. (2022). Brain injury environment critically influences the connectivity of transplanted neurons. Science Advances, 8(23), eabg9445.

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Quantifying Sca1+ Cell Uptake of PKH67+ EVs

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Four hours following the incubation of Sca1⁺ cells with PKH67⁺ EVs (10⁹ particles per 4 × 10⁵ cells) in the StemMACS media (Miltenyi Biotec), cells were stained with cell surface markers (SLAM). Cells were fixed and permeabilized with BD Cytofix/Cytoperm Plus Fixation/Permeabilization Kit and Permeabilization Buffer Plus (BD Biosciences). We used the p-Smad2 (S465/467) antibody (#18338, Cell Signaling Technology) and the secondary anti-Rabbit-AF568 antibody (Thermo Fisher Scientific). Then, SLAM cells were purified by FACS and applied on a glass slide (Superfrost plus, Thermo Fisher Scientific) for up to 10 min and fixed with ProLong Gold Antifade reagent containing DAPI (P36931, Thermo Fisher Scientific). Fluorescent images were acquired with an Axio Imager M2 (Zeiss). Fluorescent optical sections of cells were obtained under magnification ×63 using an Axio Imager M2 (Zeiss) coupled with an Apotome.2 (Zeiss). Fluorescent images were processed for study (Fiji, NIH software).

Gautheron F., Georgievski A., Garrido C, & Quéré R. (2023). Bone marrow-derived extracellular vesicles carry the TGF-β signal transducer Smad2 to preserve hematopoietic stem cells in mice. Cell Death Discovery, 9, 117.

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Microscopy Imaging of Early Embryos

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Image in Supplementary Fig. 1a (4-cell embryo) was acquired using a Plan-Apochromat ×100/1.46 Oil DIC M27 objective on a Zeiss Axio Imager.M2 equipped with a PIXIS 1024 CCD camera (Princeton Instruments). Images in Supplementary Fig 2b were acquired using a Plan-Apochromat ×40/1.0 DIC M27 objective on a Zeiss Axio Imager.M2 equipped with a PIXIS 1024 CCD camera (Pronceton Instruments).

Quarato P., Singh M., Cornes E., Li B., Bourdon L., Mueller F., Didier C, & Cecere G. (2021). Germline inherited small RNAs facilitate the clearance of untranslated maternal mRNAs in C. elegans embryos. Nature Communications, 12, 1441.

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Dendritic Structure and Spine Density Analysis

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Sections with either eGFP-expressing cells or Golgi–Cox-stained cells were visualized using a Zeiss microscope (Axio Imager M2, Zeiss) with a Hamamatsu camera (Orca-R2, Hamamatsu). Stack images with 1 µm interval on z axis were acquired (Stereology Investigator, MBF) under a ×20 objective lens. Neurolucida 360 (MBF) was used to reconstruct and analyze the dendritic structure. The total length of dendrites, nodes of dendrites, and Sholl analysis were performed. To quantitate spine density^{39 (link)}, the dendrites in the intact eGFP-expressing cells in the molecular layer of the dentate gyrus were selected and imaged. The image stacks were obtained using a Plan-Apochromat ×63 oil-immersion objective lens (NA 1.4, Zeiss) at the Zeiss Axio Imager M2 microscope with Apotome 2 with 0.25 μm z axis interval. The resulting scaling per voxel was 0.1 × 0.1 × 0.25 μm. The images were then deconvolved using Autoquant X3 (Media Cybernetics). The dendrites in the molecular layer were traced using Neurolucida 360 (MBF), and spines were identified and counted manually by the experimenter in a blinded manner.

Yu T.S., Tensaouti Y., Stephanz E.P., Chintamen S., Rafikian E.E., Yang M, & Kernie S.G. (2021). Astrocytic ApoE underlies maturation of hippocampal neurons and cognitive recovery after traumatic brain injury in mice. Communications Biology, 4, 1303.

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Multimodal Imaging of Transgenic Mice

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Except adult brain analysis, eGFP and tdTomato were detected by native fluorescence. Images are representative of at least two mice. For brain analysis, serial 60 μm thick coronal vibratome sections were generated and immunolabeled with anti-Tomato dsred as well as anti-Glial Fibrillary Acidic Protein (GFAP) antibodies, performed according to published protocols.^{25 ,36 (link)} Immunolabeled sections were counterstained with Hoechst stain. Atlas images are from the Mouse Brain Library (http://www.mbl.org/mbl_main/atlas.html).³⁷Fluorescent microscopy of slides was performed using either Zeiss LSM-700 Confocal Microscope Carl Zeiss AG, Oberkochen, Germany) (single plane) and or on a Zeiss Axio Imager M2 with respective ZEN software. Differential interference contrast microscopy of slides was performed using Zeiss Axio Imager M2. Whole-mount brightfield images were performed using an Olympus SZX12 Stereozoom microscope (Olympus Corporation, Tokyo, Japan) or Olympus MVX10 microscope. Whole mount fluorescent images were performed using an Olympus MVX10 microscope excited using an X-Cite Turbo XT600-T fluorescence light source (Excelitas Technologies Corp, Waltham, Massachusetts) at 475 and 575 nm wavelengths. Images were processed using either Canvas X (Canvas GFX), ImageJ, or Photoshop (Adobe Inc., San Jose, California).

Hagan A.S., Boylan M., Smith C., Perez-Santamarina E., Kowalska K., Hung I.H., Lewis R.M., Hajihosseini M.K., Lewandoski M, & Ornitz D.M. (2019). Generation and validation of novel conditional flox and inducible Cre alleles targeting fibroblast growth factor 18 (Fgf18). Developmental dynamics : an official publication of the American Association of Anatomists, 248(9), 882-893.

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Confocal and Wide-field Microscopy Techniques for Tissue Analysis

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The images used in this study were acquired with a Zeiss LSM 880 confocal microscope using the following objectives: Plan-Apochromat 10X 0.45 M27 (working distance 2.0 mm), and Plan-Apochromat 25X 0.8 Imm Corr DIC M27 multi-immersion. The liver images were acquired with a Keyence BZ-X700 microscope using a 20X objective. The images were then processed in the following image processing software: Zen Black 2.3 SP1 (for Zeiss confocal images) and BZ-X Analyzer (for Keyence images).
Image collection and analysis for cryosections of rat sensory ganglia and organs was performed using wide-field fluorescence microscopy with an ApoTome attachment (Zeiss AxioImager M2). Quantification of AAV+ and AAV+/Marker+ neurons was performed for DRG and trigeminal ganglia on 4 non-sequential sections with only nucleated neuronal profiles counted. Counts were performed manually while viewing sections under the microscope. For CNS sections and whole mounts of intestine and ganglia, image collection and analysis was performed using wide-field fluorescence microscopy with an ApoTome attachment (Zeiss AxioImager M2).

Chen X., Kumar S.R., Adams C.D., Yang D., Wang T., Wolfe D.A., Arokiaraj C.M., Ngo V., Campos L.J., Griffiths J.A., Ichiki T., Mazmanian S.K., Osborne P.B., Keast J.R., Miller C.T., Fox A.S., Chiu I.M, & Gradinaru V. (2022). Engineered AAVs for non-invasive gene delivery to rodent and non-human primate nervous systems. Neuron, 110(14), 2242-2257.e6.

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Immunofluorescence Analysis of Proliferative Cells

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Cells were seeded on coverslips and incubated overnight at 37°C, 5% CO₂ and 95% relative humidity. Cells attached to the surface of the microscopic slides were washed three times with PBS and fixed in 70% ethanol for 10 min. Afterwards, the samples were stained with 1:400 Hoechst diluted in PBS for 45 min. The samples were washed with PBS, embedded in Mowiol and imaged with the Zeiss Axio Imager M2. H&E stainings were done according to standard histopathological protocols. Immunofluorescence stainings of the scaffolds were performed using a primary antibody against the proliferation marker Ki67, which was incubated for 60 min at room temperature, followed by secondary antibody staining and nuclear counterstaining using Hoechst. Stained sections were examined and photographed with the Zeiss Axio Imager M2. To evaluate the impact of FLUSPIO-labeling on proliferation, the total number of cells as well as the number of proliferating Ki67-positive cells were quantified and compared. For quantifying proliferating cells in the scaffolds, 3 micrographs (100x magnification) from 3 representative sections per scaffold were analyzed. Ki67 positive cells were counted and expressed as ratio of the total cell number.

Mertens M.E., Frese J., Bölükbas D.A., Hrdlicka L., Golombek S., Koch S., Mela P., Jockenhövel S., Kiessling F, & Lammers T. (2014). FMN-Coated Fluorescent USPIO for Cell Labeling and Non-Invasive MR Imaging in Tissue Engineering. Theranostics, 4(10), 1002-1013.

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Visualizing Rhamnolipid Micelle Toxicity

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Toxicity of rhamnolipid micelles towards microorganisms was visualized using a fluorescent microscope (Zeiss Axio Imager M2). 25 μL rhamnolipid micelles were added into 75 μL 12–14 h microorganism culture in respective media. The mixture was incubated at 37°C with 225 rpm shaking for 4 h and then centrifuged at 13,300 rpm for 5 min. The pellet was resuspended in 25 μL PBS with 2 μg/mL propidium iodide for OG1RF::GFP and USA300 or 25 μL for OP50::GFP. The pellet of USA 300 was resuspended in 50 μL PBS with 40 μM acridine orange and 2 μg/mL propidium iodide. Micrographs of microorganisms were taken via Zeiss Axio Imager M2 fluorescence microscopy. Three biological replicates were performed.

Xu Q., Kang D., Meyer M.D., Pennington C.L., Gopal C., Schertzer J.W, & Kirienko N.V. (2023). Cytotoxic rhamnolipid micelles drive acute virulence in Pseudomonas aeruginosa. bioRxiv.

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