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2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts)

Manufactured by ITW Reagents
Sourced in Spain

ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)) is a chromogenic substrate used for the detection and quantification of enzyme activity, particularly peroxidase and laccase enzymes. It produces a blue-green colored product upon oxidation, which can be measured spectrophotometrically.

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4 protocols using 2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts)

1

Sustainable Hemp Waste Valorization

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Hemp agro-waste (HW) was supplied by local producers (Fundão region, Portugal). Urea, Nutrient Agar (NA), Nutrient Broth (NB), Tween 80, and sodium chloride (NaCl) were acquired from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). Choline chloride 99% (ChCl) and glycerol were purchased from Fisher Scientific (Fisher Scientific, Leicestershire, UK). Poly(vinyl alcohol) PVA (115,000 g/mol) was provided from VWR Chemicals (VWR Chemicals, Leuven, Belgium). ABTS was acquired from Panreac (Panreac, Barcelona, Spain). Potassium persulfate was supplied from Acros Organics (Acros Organics, Geel, Belgium). Phosphate-buffered saline (PBS) (pH 7.4) was purchased from Alfa Aesar (Ward Hill, MA, USA). S. aureus (ATCC 6538) and K. pneumoniae (ATCC 4352) were purchased from the American Type Culture Collection (Manassas, VA, USA).
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2

Valorizing Wool Waste for Antimicrobial Applications

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Wool waste was obtained from “A Transformadora, Lda”, a wool finishing manufacturer in Covilhã, Portugal. L-Cysteine (L-Cys), Urea, Mueller–Hinton Broth (MHB), Nutrient Agar (NA), Nutrient Broth (NB), Resazurin (7-hydroxy-3H-phenoxazin-3-one 10-oxide) dye, Tween 80, and Sodium chloride (NaCl) were purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). DL-Lactic acid 90% (LA), Choline chloride 99% (ChCl) were acquired from Fisher Scientific (Fisher Scientific, Leicestershire, UK). Poly(vinyl alcohol) PVA (115,000 g/mol) was provided from VWR Chemicals (VWR Chemicals, Leuven, Belgium). ABTS was purchased from Panreac (Panreac, Barcelona, Spain). Potassium persulfate was acquired from Acros Organics (Acros Organics, Geel, Belgium).
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3

Synthesis of Antioxidant Polymer Composites

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D-glucose anhydrous (MW = 180.16 g mol−1) and glycerol (MW = 92.09 g mol−1) were purchased from Fisher Scientific (Hampton, NH, USA). ABTS (MW = 548.69 g mol−1) was purchased from PanReac (Barcelona, Spain). Polyvinyl alcohol (PVA) (MW = 115,000 g mol−1, Viscosity = 46–54 mPa.s at 20 °C) was purchased from VWR Chemicals (Leuven, Belgium). Potassium persulfate (MW = 270.3 g mol−1) was purchased from Acros Organics (Geel, Belgium).
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4

Reagents and Materials Used in Antioxidant Analysis

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D-glucose anhydrous (MW = 180.16 g mol−1), glycerol (MW = 92.09 g mol−1), and acetic acid glacial (MW = 60.052 g mol−1) were purchased from Fisher Scientific (Hampton, NH, USA). ABTS (MW = 548.69 g mol−1), potassium peroxodisulphate (MW = 270.32 g mol−1), sodium acetate anhydrous (MW = 82.03 g mol−1), and Folin–Ciocalteu's reagent (MW = 260.20 g mol−1) were purchased from PanReac (Barcelona, Spain). Trolox (MW = 250.29 g mol−1) was purchased from ThermoScientific (Waltham, Massachusetts, USA). Gallic acid monohydrate (MW = 188.12 g mol−1) was purchased from AcrosOrganics (Geel, Belgium). Sodium carbonate anhydrous (MW = 105.99 g mol−1) was purchased from LabChem (Zelienople, Pennsylvania, US).
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