Quanta inspect f scanning electron microscope

Following incubation in presence/absence of SMF, the cell culture media was removed and the cell‐seeded microcage‐like structures were gently washed three times using PBS. Afterwards, the cells were fixed during 1 h using 2.5% glutaraldehyde in PBS. After fixation, the cells were dehydrated using ethanol (Merck, Darmstadt, Germany) solutions of successive concentration (70%‐100%), followed by ethanol‐hexamethyldisilazane (Merck, Darmstadt, Germany) (50‐50, 25‐75, 0%‐100%). The cell‐seeded mirocage‐like structures were coated with 10 nm gold and the SEM analysis was performed using the FEI Quanta Inspect F Scanning Electron Microscope (Hillsboro, OR, United States).

The Samples’ Surface Morphology and Elemental Composition were examined using a QUANTA INSPECT F Scanning Electron Microscope (FEI Company, Eindhoven, The Netherlands) coupled with an Energy-Dispersive X-Ray Spectrometer Detector (EDAX).

Three random specimens from each group were selected for SEM investigation (n = 12) and examined by a QUANTA INSPECT F Scanning Electron Microscope (FEI Company, Eindhoven, the Netherlands), working at an acceleration voltage of up to 30 kV and equipped with an energy-dispersive X-ray spectrometer detector (EDAX) with a 132 eV resolution at Mn-Kα. Specimens were dried, mounted on aluminum stubs, placed in sealed glass vials with silica, and sputter-coated with gold in a vacuum chamber. In order to analyze the dentin–sealer–core interface, serial SEM photomicrographs were taken at different magnifications (40× and 4000×). Two evaluations were performed for each photomicrograph.