Urea nitrogen test kit

Manufactured by Nanjing Jiancheng

Sourced in China

The Urea Nitrogen Test Kit is a laboratory equipment used to measure the concentration of urea nitrogen in a sample. It provides a quantitative analysis of urea nitrogen levels without interpretation or extrapolation of the intended use.

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Urea nitrogen kit (3 citations) Urea (2 citations) Blood urea nitrogen test kit (2 citations)

5 protocols using urea nitrogen test kit

Automated Biochemical Analysis of Murine Urinary Protein

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A fully automatic biochemical analyzer was used to determine the urinary protein of mice at 13 weeks old (24 h before treatment) and at 25 weeks old (one day before the end of treatment). Blood was collected from the abdominal aorta and sit at room temperature for 2 h. After centrifuged at 1500 rpm for 10 min, the supernatant was collected. The urine creatinine test kit (Nanjing Jiancheng, C011-1) and urea nitrogen test kit (Nanjing Jiancheng, C013-2) were used to test the specific biofluid of interest, urine, pladma, and serum according to manufacturer’s instructions.

Yuan S., Liang X., He W., Liang M., Jin J, & He Q. (2021). ATF4-dependent heme-oxygenase-1 attenuates diabetic nephropathy by inducing autophagy and inhibiting apoptosis in podocyte. Renal Failure, 43(1), 968-979.

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HPLC-based Metabolite Extraction and Analysis

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High performance liquid chromatography (HPLC) grade acetonitrile and methanol were purchased from Thermo Fisher Scientific Co., Ltd. (Fair Lawn, NJ, USA). Ultra-pure water was obtained from Hangzhou Wahaha Group Co., Ltd. (Hangzhou, China), N,O-bis(trimethylsilyl)trifluoro-acetamide (BSTFA) with 1% trimethylchlorosilane (TMCS), O-methoxyamine-HCl (MOX), succinic-d₄ acid were purchased from Sigma–Aldrich (NJ, USA). Adenine, 4% tissue cell fixative, yeast powder, urease activity test kit, hematoxylin-eosin staining (HE) staining kit, Masson staining kit and standards for creatinine, uric acid, hypo xanthine, xanthine, urea, etc. were purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China) and the purity of the above standands are all greater than 98%. The urea nitrogen test kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Other chromatographic reagents were obtained from domestic reagent companies.

Pan L., Han P., Ma S., Peng R., Wang C., Kong W., Cong L., Fu J., Zhang Z., Yu H., Wang Y, & Jiang J. (2019). Abnormal metabolism of gut microbiota reveals the possible molecular mechanism of nephropathy induced by hyperuricemia. Acta Pharmaceutica Sinica. B, 10(2), 249-261.

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Albumin and Urea Production Kinetics

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After 5, 10, 15, and 20 days in the culture systems, culture supernatants were collected, the albumin content was measured using a human albumin ELISA kit (Solarbio, Beijing, China), and the urea content was measured using a urea nitrogen test kit (Nanjing Jiancheng, Nanjing, China). The albumin data are expressed as ng/10⁴ cells·h, and the urea data are expressed as μg/10⁴ cells·h.

Liu Y., Ge Y., Wu Y., Feng Y., Liu H., Cao W., Xie J, & Zhang J. (2024). High-Voltage Electrostatic Field Hydrogel Microsphere 3D Culture System Improves Viability and Liver-like Properties of HepG2 Cells. International Journal of Molecular Sciences, 25(2), 1081.

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Measuring BUN and Serum Creatinine

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The blood urea nitrogen (BUN) levels and serum creatinine (Scr) levels were measured using urea nitrogen test kits and creatinine assay kits (Jiancheng Bioengineering Institute, Nanjing, China). The experimental procedures outlined in the instruction manual were followed to determine the blood urea nitrogen and creatinine content.

Yu D., Huang W., Sheng M., Zhang S., Pan H., Ren F., Luo L., Zhou J., Huang D, & Tang L. (2024). Angiotensin-(1-7) Modulates the Warburg Effect to Alleviate Inflammation in LPS-Induced Macrophages and Septic Mice. Journal of Inflammation Research, 17, 469-485.

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Biomarkers of Kidney Function

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Five mice from each group were killed at the same point by exsanguination under anesthesia before blood samples were collected, followed by centrifuging blood at 3000 rpm for 10 min at 4°C to obtain serum. Urea nitrogen and creatinine were detected using urea nitrogen test kits and creatinine test kits (Nanjing Jiancheng Bioengineering Research Institute, China), respectively. Microalbuminuria was detected using mouse urine microalbuminuria detection kits (Shanghai Enzyme-linked Biotechnology Co., Ltd., China).

Zhou X.C., Liang Y.J., Qin L., Wei G.H, & Wang J.Q. (2021). Astragalus–Saffron–Rhubarb Mixture Delays the Progress of Diabetic Nephropathy in db/db Mice. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 14, 4679-4690.

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