Mqx200

Manufactured by Agilent Technologies

Sourced in United States

The MQX200 is a multipurpose lab equipment designed for various analytical applications. It features a modular design and can be configured with various components to meet specific research or testing requirements. The core function of the MQX200 is to provide a versatile platform for scientific analysis and experimentation.

Automatically generated - may contain errors

Lab products found in correlation

Cck 8 assay kit, by Dojindo Laboratories (6 mentions) Cck 8, by Dojindo Laboratories (2 mentions) Microplate reader, by Thermo Fisher Scientific (2 mentions) Cck 8 assay kit, by Biosharp (1 mentions) Mts assay, by Promega (1 mentions) Cell counting kit 8 (cck8), by Beyotime (1 mentions) Cck 8, by Agilent Technologies (1 mentions) Cell counting kit 8 cck 8, by Merck Group (1 mentions) Cck 8 detection kit, by Dojindo Laboratories (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Cell counting kit 8 (cck8), by Merck Group (1 mentions) Cck 8, by Merck Group (1 mentions) Cck 8, by Targetmol (1 mentions) Griess reagent kit, by Beyotime (1 mentions) Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions) Cell counting kit 8 (cck8), by Keygen Biotech (1 mentions) Elisa kit, by BioLegend (1 mentions) Metformin, by Merck Group (1 mentions) Cck 8 solution, by Dojindo Laboratories (1 mentions) Cck 8 reagent, by Beyotime (1 mentions)

53 protocols using mqx200

Cytotoxicity and Proliferation Assay of BBR

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The CCK‐8 assay kit (Dojindo) was used for testing cytotoxicity in vitro. At PD45, 2BS or WI38 cells were seeded into flat‐bottomed 96‐well microplates at a density of 5 × 10³ cells/0.2 ml per well. After 20 hr, when the cells reached a subconfluent state, the cells were transferred to a special culture medium containing various concentrations of BBR for further growth, at 37°C in 5% CO₂ up to 24 hr. Then, the CCK‐8 solution (diluted 0.1 times with 10% FBS DMEM) was added to each well and the cells were incubated for 1 hr at 37°C. The absorbance values of each well were determined spectrophotometrically at 490 nm using a microplate reader (Biotek, MQX200).
Cell viability (%) = (OD _{treatment group} − OD _blank)/ (OD_{control group} − OD _blank) × 100.
Cell proliferation was assayed using the CCK‐8 method. Cells were seeded into 96‐well plates at 2.5 × 10³ cells/0.2 ml per well and incubated with different concentrations of BBR (0, 0.3125, 1.25, and 5 μg/ml) for one week. The absorbance values of each well were measured at day 0 (4 hr after plating) and on days 1, 2, 3, 4, 5, and 6. The medium containing BBR or DMSO was refreshed every 24 hr. At the indicated points, cells were harvested with 10% CCK‐8 for one hour, and the absorbance was measured at 490 nm. Each data point was measured five times, and each curve was repeated more than three times.

Dang Y., An Y., He J., Huang B., Zhu J., Gao M., Zhang S., Wang X., Yang B, & Xie Z. (2019). Berberine ameliorates cellular senescence and extends the lifespan of mice via regulating p16 and cyclin protein expression. Aging Cell, 19(1), e13060.

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Cell Proliferation Assay with CCK-8

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A CCK-8 assay kit (Dojindo, Tokyo, Japan) was used to evaluate the cell proliferation of MDCK cells. Cells were plated in 96-well plates at a density of 1 × 10⁴ cells/well. At each time point, 100 μL CCK-8 solution diluted 1/10 with DMEM containing 10% FBS was added to each well, and the cells were incubated for 2 h at 37 °C. The absorbance at 450 nm was measured with a microplate reader (MQX200; BioTek Instruments, Winooski, VT, USA).

Meng J., Sai-zhen W.a.n.g., He J.Z., Zhu S., Huang B.Y., Wang S.Y., Li M., Zhou H., Lin S.Q, & Yang B.X. (2020). Ganoderic acid A is the effective ingredient of Ganoderma triterpenes in retarding renal cyst development in polycystic kidney disease. Acta Pharmacologica Sinica, 41(6), 782-790.

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Antioxidant Reducing Power Assay

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Antioxidants reduced potassium ferricyanide (K₃Fe(CN)₆) to potassium ferrocyanide (K₄Fe(CN)₆), and potassium ferrocyanide reacted with Fe3⁺ to form Prussian blue. Three hundred microliters of varying concentrations of sample solution were added to 300 μL of 0.2 M potassium phosphate buffer (pH 6.6) and 300 μL of 1% K₃Fe(CN)₆ for 20 min at 50 °C. After cooling to room temperature, 300 μL of 10% TCA was added and centrifuged at 3000 rpm for 20 min. The supernatant was added to 500 μL of distilled water and 100 μL of 0.1% FeCl₃. The mixing solutions were incubated in the dark for 10 min and the absorbance at 700 nm was recorded by an ELISA-reader (MQX-200, BioTek, Winooski, VT, USA). All determinations were carried out in triplicate.
The reducing power was calculated by Equation (4):

Reducing power = Ai - A 0

where A0 is the absorbance of blank; Ai is the absorbance of the sample.

Wu P.S., Li Y.S., Kuo Y.C., Tsai S.J, & Lin C.C. (2019). Preparation and Evaluation of Novel Transfersomes Combined with the Natural Antioxidant Resveratrol. Molecules, 24(3), 600.

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Evaluating Cytotoxicity of RSV Transfersomes

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The cell line used in this experiment was B16-F10 (BCRC 60031) mouse melanoma cells purchased from the Food Industry Research and Development Institute (Hsinchu, Taiwan). B16-F10 cells were seeded at a density of 6 × 10³ cell/well in a 96-well plate and maintained in 100 µL Dulbecco’s modification of Eagle medium (DMEM) supplemented with 10% PBS for 24 h in a humidified incubator (37 ^OC, 5% CO₂). Each well was then treated with RSV and RSV transfersomes at serial concentrations. After being cultured for 24 h, 100 µL (1.0 mg/mL) of MTT solution was added into each well for 30 min to allow the formation of formazan crystal. Subsequently, supernatant was removed carefully and 100 µL DMSO was added to each well. Cell viabilities were evaluated using an ELISA-reader (MQX-200, BioTek, Winooski, VT, USA) at 540 nm.

Wu P.S., Li Y.S., Kuo Y.C., Tsai S.J, & Lin C.C. (2019). Preparation and Evaluation of Novel Transfersomes Combined with the Natural Antioxidant Resveratrol. Molecules, 24(3), 600.

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Evaluating Cardioprotective Effects of XKS

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For this experiment, rat fetal cardiomyocytes (H9c2) human hepato were obtained from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. H9c2 cells were grown in CM under humid atmosphere at37 °C in CO₂ incubator. Cell cultures between passages 3 to 5 were used for each experiment.
The cultured H9c2 cells were detached by trypsinization, centrifuged at 1000 rpm for 5 min and resuspended in fresh CM at a density of 5 × 10⁴ cells/mL. Then, 100 μL of the cells were planted onto 96-well flat bottom plates. After incubation in 5% CO₂-air mixture at 37 °C for 24 h, the cells were pretreated with the XKS (0.25, 0.125 and 0.0625 mg/mL) and cultured at 37 °C for an additional 24 h, then ISO (15 μM) was added into the plate and cultured at 37 °C for an additional 24 h. and the cancer cell without drugs and ISO was used as control. 20 mL of MTT stock solution (5 mg/mL in PBS without phenol red in the dark and filtered through a 0.2 μm filter before use) was then added into each well. After incubating for 4 h at 37 °C, 150 μL DMSO was added to each well to dissolve the formazan crystals. Then the plates were gently shaken for 1 min and determined by a microplate reader (Thermo, USA) at 490 nm. The morphology of the cells was evaluated by inverted microscope (MQX 200, BioTek, USA).

Liu Y.T., Zhou C., Jia H.M., Chang X, & Zou Z.M. (2016). Standardized Chinese Formula Xin-Ke-Shu inhibits the myocardium Ca2+ overloading and metabolic alternations in isoproterenol-induced myocardial infarction rats. Scientific Reports, 6, 30208.

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Renal Tubular Cell Cytotoxicity Assay

Cited 3 times

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Logarithmic renal tubular epithelial cells (NRK52E cell line was derived from Shanghai Cell Bank, Chinese Academy of Sciences) were cultured as described previously [37 (link)]. Cytotoxicity was detected using a CCK-8 assay kit (BS350B, Biosharp, Hefei, China). Cells were plated in 96-well plates at a density of 8000 cells/well. After incubation with AAI (0, 5, 10, 20, 30, 35, 40, 80 μM) and LYC (0, 2.5, 5, 10, 20, 40 μM) for 24 h, 10% CCK-8 solution diluted in serum-free DMEM was added to each well and incubated at 37 °C for 2 h. The absorbance at 450 nm was measured by a microplate reader (Biotek, MQX200, Winooski, VT, USA) [38 (link)]. The optimal dosing concentrations of AAI and LYC were selected for subsequent experiments. The experiment was divided into five groups: control group(Con), model group (AAI 40 μM)(AAI), LYC control group (LYC 10 μM)(LYC), LYC high and low dose treatment groups (LYC 5 μM and 10 μM)(AL5 AL10), cells were treated with AAI (40 μM) and pretreated with Nrf2 inhibitor ML385 (5 μM) and Nrf2 activator AKBA (40 μM) [39 ,40 (link)], respectively, and the relevant parameters were measured after 24 h.

Wang Y., Liu Z., Ma J., Xv Q., Gao H., Yin H., Yan G., Jiang X, & Yu W. (2022). Lycopene attenuates the inflammation and apoptosis in aristolochic acid nephropathy by targeting the Nrf2 antioxidant system. Redox Biology, 57, 102494.

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Penfluridol Cytotoxicity Assay in AML Cells

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AML cells at a density of 3 × 10⁴ cells/well were seeded into 24-well culture plates containing complete media and incubated for 1 day, after which they were exposed to different concentrations of penfluridol for the indicated time points, and cell viabilities were determined by an MTS assay (Promega, Madison, WI). The absorbance (A) was read at 490 nm using an enzyme-linked immunosorbent assay (ELISA) reader (MQX200; Bio-Tek Instruments, Winooski, VT). The cell viability rate (%) was analyzed by the formula: A_{490, penfluridol}/A_{490, vehicle} × 100, and the half maximal inhibitory concentration (IC₅₀) was further determined.

Wu S.Y., Wen Y.C., Ku C.C., Yang Y.C., Chow J.M., Yang S.F., Lee W.J, & Chien M.H. (2019). Penfluridol triggers cytoprotective autophagy and cellular apoptosis through ROS induction and activation of the PP2A-modulated MAPK pathway in acute myeloid leukemia with different FLT3 statuses. Journal of Biomedical Science, 26, 63.

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Evaluating Cardioprotective Agents

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To select the appropriate propofol and ropivacaine concentrations, cell viability was assessed using the Cell Counting Kit-8 (CCK-8; Beyotime Institute of Biotechnology) according to the manufacturer's instruction. Cardiomyocytes were seeded at a density of 3×10³ cells in 96-well plates in sextuplicate and incubated overnight in the complete DMEM/F12 with or without 500 µmol/l CoCl₂ in an atmosphere with 5% CO₂ at 37°C. After removing the culture medium, cells were treated with propofol (100, 50, 25, 12.5, 6.25 and 0 µg/ml) or ropivacaine at different concentrations (300, 150, 75, 37.5, 18.75 and 0 µg/ml in the absence of Cocl2 treatment experiments; 100, 50, 25, 12.5 and 0 µg/ml in the 500 µmol/l Cocl2-pretreatment experiments) in fresh medium, repeated eight times, for 48 h at 37°C. In addition, to determine the synergistic interactions between propofol and ropivacaine, AC16 and HCM cells were treated by 25 µg/ml of propofol and different ropivacaine concentrations (200, 100, 50, 25, 12.5 and 0 µg/ml) for 48 h at 37°C. The supernatants were discarded and the CCK-8 solution, (dilution, 1:10) was added to each well in complete DMEM/F12, and the cells were incubated for 2 h at 37°C. Absorbance at 450 nm was measured using a microplate reader (cat. no. MQX200; BioTek Instruments, Inc.). All experiments were repeated four times.

Han L., Zhuo Q., Zhou Y, & Qian Y. (2020). Propofol protects human cardiac cells against chemical hypoxiainduced injury by regulating the JNK signaling pathways. Experimental and Therapeutic Medicine, 19(3), 1864-1870.

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Tricetin's Cytotoxic Effects on AML Cells

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Four AML cells (MV4-11, HL-60, U937, and THP-1) were cultured in 96-well plates containing complete media and treated with different concentrations of tricetin (0, 20, 40, 80, and 160 μM) for 24 h, and cell viabilities were examined using a Cell Counting Kit-8 (CCK-8) (Sigma-Aldrich) or MTS (Promega, Madison, WI, USA) assay. The absorbance (A) was read at 450 nm (CCK-8 assay) or 490 nm (MTS assay) using an enzyme-linked immunosorbent assay (ELISA) reader (MQX200; Bio-Tek Instruments, Winooski, VT, USA). The cell viability rate (multiple) was analyzed by the formula: A_{450 or 490, tricetin}/A_{450 or 490, vehicle}.

Chien M.H., Chow J.M., Lee W.J., Chen H.Y., Tan P., Wen Y.C., Lin Y.W., Hsiao P.C, & Yang S.F. (2017). Tricetin Induces Apoptosis of Human Leukemic HL-60 Cells through a Reactive Oxygen Species-Mediated c-Jun N-Terminal Kinase Activation Pathway. International Journal of Molecular Sciences, 18(8), 1667.

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Cytocompatibility Assessment of Osteoblast Cells

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To evaluate cytocompatibility,
the mouse osteoblast cells were
cultured at 37 °C and a 5% CO₂ incubator in α-MEM
supplemented with 10% FBS and 1% penicillin–streptomycin. The
culture medium was changed every 2 days.
Cells were seeded with
at a density of 1 × 10⁵ cells/mL on the surface of
the sample in a 24-well plate. After incubating with the sample for
12 h, 24 h and 3 days, 40 μL of the MTT reagent was added to
each well and reacted for 3 h. Samples were removed and placed in
a new 24-well plate, 200 μL of DMSO solutions was added to each
well followed by reaction for 10 min, 150 μL of mixed solution
was transferred to a 96-well plate, and the absorbance was detected
using a microplate reader (Bio-tek MQX200) at 490 nm. In addition,
at each point in time, another sample of every group were removed
and rinsed with PBS. The cells were fixed with 1% glutaraldehyde for
2 h. Then, rhodamine 123 was used to stain the cells for 10 min in
the dark, and then, samples were observed with a fluorescence microscope.

Guo C., Cui W., Wang X., Lu X., Zhang L., Li X., Li W., Zhang W, & Chen J. (2020). Poly-l-lysine/Sodium Alginate Coating Loading Nanosilver for Improving the Antibacterial Effect and Inducing Mineralization of Dental Implants. ACS Omega, 5(18), 10562-10571.

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