Anti rabbit or mouse secondary antibodies for immunoblots horse radish peroxidase conjugated with igg

RNeasy kit, reverse transcription kit, and QuantiTect SYBR Green PCR kit were purchased from Qiagen Inc., Valencia, CA. The antibodies for GCLM, GCLC, NQO1, GSR, GSTµ, SOD1, and SOD2 procured from Abcam, Cambridge, MA, USA, Catalase-ab from EMD Millipore, USA and rabbit anti-GAPDH from Cell Signaling, USA. The anti-rabbit or mouse secondary antibodies for immunoblots horse radish peroxidase conjugated with IgG were purchased from Vector Laboratories, Burlingame, CA, USA. Secondary antibodies for immunofluorescence conjugated with Alexa fluor 488 anti-rabbit and anti-mouse were obtained from Life Technologies Corporation, NY, USA. Primers for qPCR were designed using Harvard medical school PrimerBank website and purchased from Integrated DNA Technologies, Coralville, IA. Protein Assay reagent (#500–0006) was procured from Bio-Rad, Hercules, CA. All other chemicals including reduced and oxidized glutathione were purchased from Sigma-Aldrich unless otherwise stated.