Cells were resuspended in staining buffer (BD biosciences). After being fixed and penetrated, cells were stained with PE-CD3 (130-117-139, 1:50, Miltenyi Biotec) and Pacific blue-IFNγ (BioLegend, #505817, Rat, 1:50). Samples were analyzed by flow cytometry (BD Immunocytometry System) and FlowJo software [29 (link)].
Pacific blue ifnγ
Manufactured by BioLegend
Sourced in United States, Germany
Pacific blue-IFNγ is a fluorochrome-conjugated antibody that binds to interferon-gamma (IFNγ). It can be used for the detection and quantification of IFNγ-producing cells in flow cytometry applications.
Automatically generated - may contain errors
Lab products found in correlation
Staining buffer, by BD (3 mentions)
Pe cd3 130 117 139, by Miltenyi Biotec (2 mentions)
Cd3 percp, by BioLegend (2 mentions)
Facscanto 2, by BD (1 mentions)
Pd l1 apc, by BioLegend (1 mentions)
Anti mouse pd 1, by BioXCell (1 mentions)
Af700cd45, by BioLegend (1 mentions)
Cd62l pe cy7, by BioLegend (1 mentions)
Percp cy5.5 pd 1, by BioLegend (1 mentions)
Fixation permeabilization kit, by BD (1 mentions)
Cd44 apc cy7, by BioLegend (1 mentions)
Cd8a apc, by BioLegend (1 mentions)
Live dead dye, by Thermo Fisher Scientific (1 mentions)
Cd4 fitc, by BioLegend (1 mentions)
Brefeldin a golgiplug, by BD (1 mentions)
Bv605 tnfα, by BioLegend (1 mentions)
Anti mouse ctla 4, by BioXCell (1 mentions)
Collagenase type 1, by Thermo Fisher Scientific (1 mentions)
Dnase 1, by Roche (1 mentions)
Fc block, by BD (1 mentions)
5 protocols using pacific blue ifnγ
1
Flow Cytometry Analysis of IFNγ-expressing T cells
2
Flow Cytometric Analysis of T Cell Activation
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The cells were made into a single-cell suspension and resuspended in staining buffer (BD Biosciences, Franklin Lakes, NJ, USA). T cells were cultured with PerCP-CD3 (1:100, BioLegend, San Diego, CA, USA, #100326, Armenian hamster) and fixed and permeabilized with Pacific blue-IFN-γ (1:50, BioLegend, San Diego, CA, USA, #505817, rat). Subsequently, the cells were detected with a BD FACS Canto II flow cytometer (BD Immunocytometry Systems, San Jose, CA, USA) and analyzed with FlowJo software.
3
Quantifying PD-L1 and IFNγ in Cells
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The cells were made into single-cell suspension, and resuspended in dye buffer solution (BD Biosciences, Franklin Lakes, NJ, United States). AGS cells were incubated with APC-PD-L1 (#329708, Mouse, 1:50, Biolegend, San Diego, CA, United States). The T cells were incubated with PerCP-CD3 (#100326, Armenian Hamster, 1:100 Biolegend) and Pacific blue-IFNγ (#505817, Rat, 1:50, Biolegend) that was pre-fixed and pre-permeabilized. Cell apoptosis was assessed using a flow cytometer BD FACS CantoII (BD Immunocytometry Systems, Franklin Lakes, NJ, United States) and analyzed by Flow Jo software.
4
Peptide Library Synthesis and Liposome Formulation
5
Flow Cytometry Analysis of IFN-γ in Jurkat T Cells
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The cells were dispersed into a single-cell suspension and resuspended in staining buffer (BD, New Jersey, USA) while T cells (Jurkat) were treated with PE-CD3 (130-117-139, 1 : 50, Miltenyi Biotec, Bergisch Gladbach, Germany), fixed, permeabilized, and stained with Pacific blue-IFN-γ (BioLegend, San Diego, CA, #505817, rat, 1 : 50), followed by detection utilizing flow cytometer BD FACS Canto II (Immunocytometry Systems, BD, New Jersey). Acquired data were analyzed by the Flow Jo software [21 (link)].
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