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Viia7 icycler real time sybr pcr detection system

Manufactured by Thermo Fisher Scientific
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The ViiA7 iCycler real-time SYBR PCR detection system is a laboratory instrument designed for real-time polymerase chain reaction (PCR) analysis. It is capable of detecting and quantifying SYBR Green fluorescent dye-based PCR amplification in real-time.

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Lab products found in correlation

Iscript cdna synthesis kit, by Bio-Rad (3 mentions) Trizol reagent, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

ViiA7 (579 citations) ViiA7 PCR system (53 citations) ViiA7 real-time PCR (27 citations) Real-Time System (23 citations) ViiA7 detection system (19 citations) ICycler (17 citations) ICycler PCR (2 citations)

3 protocols using viia7 icycler real time sybr pcr detection system

1

Quantitative Analysis of PAX8 Expression

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Cells were grown to 80% confluency before harvest in Trizol reagent
(Life Technologies, Carlsbad, CA) and RNA isolation was performed according to
the manufacturer’s protocol. Total RNA (1μg) was converted to cDNA
using the iScript cDNA synthesis kit (BioRad, Hercules, CA). cDNA was amplified
using a ViiA7 iCycler real-time SYBR PCR detection system (Life Technologies,
Carlsbad, CA). The PAX8 primers were designed to span exon-exon junctions to
prevent binding to genomic DNA. Samples were normalized to the housekeeping
gene, RNA18S. Three biological replicates were performed.
Hardy L.R., Pergande M.R., Esparza K., Heath K.N., Önyüksel H., Cologna S.M, & Burdette J.E. (2019). Proteomic analysis reveals a role for PAX8 in peritoneal colonization of high grade serous ovarian cancer that can be targeted with micelle encapsulated thiostrepton. Oncogene, 38(32), 6003-6016.
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2

Quantitative Analysis of PAX8 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were grown to 80% confluency before harvest in Trizol reagent
(Life Technologies, Carlsbad, CA) and RNA isolation was performed according to
the manufacturer’s protocol. Total RNA (1μg) was converted to cDNA
using the iScript cDNA synthesis kit (BioRad, Hercules, CA). cDNA was amplified
using a ViiA7 iCycler real-time SYBR PCR detection system (Life Technologies,
Carlsbad, CA). The PAX8 primers were designed to span exon-exon junctions to
prevent binding to genomic DNA. Samples were normalized to the housekeeping
gene, RNA18S. Three biological replicates were performed.
Hardy L.R., Pergande M.R., Esparza K., Heath K.N., Önyüksel H., Cologna S.M, & Burdette J.E. (2019). Proteomic analysis reveals a role for PAX8 in peritoneal colonization of high grade serous ovarian cancer that can be targeted with micelle encapsulated thiostrepton. Oncogene, 38(32), 6003-6016.
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3

Relative Gene Expression Analysis

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Total RNA (1.0 μg) was reverse transcribed using the iScript cDNA synthesis kit (BioRad), according to manufacturer’s instructions. cDNA was amplified and analyzed using ViiA7 iCycler real-time SYBR PCR detection system (Life Technologies). Samples are normalized relative to the internal control Gapdh and MOEHIGH cells were compared to the MOELOW cells (ΔΔCt expressions) of Cdkn1a, Foxm1, Trp53, Ovgp1, and Pax8.
Endsley M.P., Moyle-Heyrman G., Karthikeyan S., Lantvit D.D., Davis D.A., Wei J.J, & Burdette J.E. (2015). Spontaneous Transformation of Murine Oviductal Epithelial Cells: A Model System to Investigate the Onset of Fallopian-Derived Tumors. Frontiers in Oncology, 5, 154.
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