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Recombinant human rhu ccl21

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Recombinant human (rhu)-CCL21 is a protein produced in a human cell expression system. It is a member of the chemokine (C-C motif) ligand 21 family. Chemokines are a family of small cytokines or proteins secreted by numerous cell types and have the ability to induce directed chemotaxis in nearby responsive cells.

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Lab products found in correlation

Rhu il 4, by Thermo Fisher Scientific (4 mentions) Cd80 fitc, by BD (4 mentions) Cd86 fitc, by BD (4 mentions) Fitc mouse igg1, by BioLegend (4 mentions) Pe mouse igg1, by BioLegend (4 mentions) Cd40 pe, by BioLegend (4 mentions) Cd83 pe, by BD (4 mentions) Purified rat igg2a, by BioLegend (4 mentions) Goat anti rat igg pe, by BioLegend (4 mentions) Cd14 pe, by BD (3 mentions) E toxate kit, by Merck Group (3 mentions) Cd1a pe, by BD (3 mentions) Langerin pe, by BD (3 mentions) Rhu tgfβ1, by Thermo Fisher Scientific (3 mentions) Cd40 pe, by BD (2 mentions) Hla abc fitc, by BD (2 mentions) Hla dp, by BD (2 mentions) Purified anti human ccr7, by BD (2 mentions) Tlr3 pe, by BD (2 mentions) Cd8 fitc, by BD (1 mentions)

Spelling variants of this product

CCL21 (67 citations) Recombinant human CCL21 (4 citations) Recombinant CCL21 (2 citations)

4 protocols using recombinant human rhu ccl21

1

Multiparameter Immune Cell Profiling

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HLA-ABC FITC, HLA-DP,DQ,DR-FITC, CD40 PE, CD80 FITC, CD86 FITC, CD83 PE, CD1a PE, purified anti-human CCR7, CD14 PE, Langerin PE, TLR3 PE were purchased from BD Biosciences (San Jose, CA). CD40 PE, purified rat IgG2a, goat anti-rat IgG PE, mouse IgG1 FITC, mouse IgG1 PE were purchased from Biolegend (San Diego, CA). Recombinant human (rhu)-CCL21 was purchased from R&D Systems (Minneapolis, MN). Rhu-GM-CSF was manufactured by Berlex (Seattle, WA) while rhu-TGFβ1 and rhu-IL-4 were purchased from Biosource (Carlsbad, CA). Poly-ICR was provided by Nventa Biopharmaceuticals/Akela Pharma (Austin, TX). Poly-ICLC was provided by Oncovir, Inc. (Washington, DC) HPV16L1L2 virus-like particles (VLP) and chimeric HPV16L1L2-E7 VLP (HPV16 cVLP) were produced in insect cells and purified as previously described [28] (link). Endotoxin levels in VLP preparations were found to be below 0.06 EU using an E-toxate kit (Sigma-Aldrich).
Da Silva D.M., Woodham A.W., Rijkee L.K., Skeate J.G., Taylor J.R., Koopman M.E., Brand H.E., Wong M.K., McKee G.M., Salazar A.M, & Kast W.M. (2015). Human papillomavirus-exposed Langerhans cells are activated by stabilized Poly-I:C. Papillomavirus Research, 1, 12-21.
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2

Characterization of Dendritic Cell Phenotype

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HLA-ABC FITC (MHC I), HLA-DP, DQ, DR-FITC (MHC II), CD80 FITC, CD86 FITC, CD83 PE, CD1a PE, CD14 PE, Langerin PE, E-cadherin PE, CD8-FITC, CD3-PE-Cy5, and CCR7 PE were purchased from BD Biosciences (San Jose, CA). CD40 PE, purified rat IgG2a, goat anti-rat IgG PE, mouse IgG1 FITC, and mouse IgG1 PE were purchased from Biolegend (San Diego, CA). Recombinant human (rhu)-CCL21 was purchased from R&D Systems (Minneapolis, MN); rhu-GM-CSF was purchased from Berlex (Seattle, WA); and rhu-transforming growth factor-β1 (TGFβ1) and rhu-IL-4 were purchased from Biosource (Carlsbad, CA).
Da Silva D.M., Woodham A.W., Naylor P.H., Egan J.E., Berinstein N.L, & Kast W.M. (2016). Immunostimulatory Activity of the Cytokine-Based Biologic, IRX-2, on Human Papillomavirus-Exposed Langerhans Cells. Journal of Interferon & Cytokine Research, 36(5), 291-301.
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3

Comprehensive Dendritic Cell Immunophenotyping

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HLA-ABC FITC (MHC I), HLA-DP,DQ,DR FITC (MHC II), CD80 FITC, CD83 PE, CD86 FITC, and purified anti-human CCR7, were purchased from BD Biosciences (San Jose, CA). CD40 PE, purified rat IgG2a, goat anti-rat IgG PE, mouse IgG1 FITC, mouse IgG1 PE were purchased from Biolegend (San Diego, CA). Recombinant human (rhu)-CCL21 was purchased from R&D Systems (Minneapolis, MN), Rhu-GM-CSF from Berlex (Seattle, WA), and rhu-TGFβ1 and rhu-IL-4 from Biosource (Carlsbad, CA). Poly-ICR was provided by Nventa Biopharmaceuticals/Akela Pharma (Austin, TX). Poly-ICLC (Hiltonol®) is a clinical grade current good manufacturing practices (cGMP) form of s-Poly I:C that was provided by Oncovir, Inc. (Washington, D.C.) HPV16 L1L2 virus-like particles (VLP) and chimeric HPV16 L1L2-E7 VLP (HPV16 cVLP) were produced in insect cells and purified following published procedures [28 (link)], and endotoxin levels were below 0.06 EU as measured using an E-toxate kit (Sigma-Aldrich).
DA SILVA D.M., WOODHAM A.W., SKEATE J.G., RIJKEE L.K., TAYLOR J.R., BRAND H.E., MUDERSPACH L.I., ROMAN L.D., YESSAIAN A.A., PHAM H.Q., MATSUO K., LIN Y.G., McKEE G.M., SALAZAR A.M, & KAST W.M. (2015). Langerhans cells from women with cervical precancerous lesions become functionally responsive against human papillomavirus after activation with stabilized Poly-I:C. Clinical immunology (Orlando, Fla.), 161(2), 197-208.
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4

Phenotypic Characterization of Dendritic Cells

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HLA-ABC FITC, HLA-DP, DQ, DR-FITC, CD40 PE, CD80 FITC, CD86 FITC, CD83 PE, CD1a PE, purified anti-human CCR7, CD14 PE, Langerin PE, TLR3 PE were purchased from BD Biosciences (San Jose, CA). CD40 PE, purified rat IgG2a, goat anti-rat IgG PE, mouse IgG1 FITC, mouse IgG1 PE were purchased from Biolegend (San Diego, CA). Recombinant human (rhu)-CCL21 was purchased from R&D Systems (Minneapolis, MN). Rhu-GM-CSF was manufactured by Berlex (Seattle, WA) while rhu-TGFβ1 and rhu-IL-4 were purchased from Biosource (Carlsbad, CA). Poly-ICR was provided by Nventa Biopharmaceuticals/Akela Pharma (Austin, TX). Poly-ICLC was provided by Oncovir, Inc. (Washington, D.C.) HPV16L1L2 virus-like particles (VLP) and chimeric HPV16L1L2-E7 VLP (HPV16 cVLP) were produced in insect cells and purified as previously described [28 (link)]. Endotoxin levels in VLP preparations were found to be below 0.06 EU using an E-toxate kit (Sigma-Aldrich).
Da Silva D.M., Woodham A.W., Rijkee L.K., Skeate J.G., Taylor J.R., Koopman M.E., Brand H.E., Wong M.K., McKee G.M., Salazar A.M, & Kast W.M. (2015). Human papillomavirus-exposed Langerhans cells are activated by stabilized Poly-I:C. Papillomavirus Research, 1, 12-21.
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