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10r g109a

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10R-G109A is a lab equipment product. It is a scientific instrument used for research and analysis purposes. The core function of this product is to perform specific tasks in a laboratory setting. No further details on the intended use or capabilities of this product can be provided in an unbiased and factual manner.

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Lab products found in correlation

Calreticulin, by Cell Signaling Technology (2 mentions) Rapamycin, by Merck Group (2 mentions) Ab8983, by Merck Group (2 mentions) Ab16123, by Merck Group (2 mentions) Cox 4, by Cell Signaling Technology (2 mentions) Lamins a c, by Merck Group (2 mentions) Mab3211, by Roche (2 mentions) H3663, by Active Motif (2 mentions) H4a3 s, by Merck Group (2 mentions) H3k27me3, by Active Motif (2 mentions) β tubulin, by Merck Group (2 mentions) H3k9me3, by Abcam (2 mentions) Flag tag (flag), by Merck Group (2 mentions) 4 hydroxytamoxifen, by Merck Group (2 mentions) Etoposide, by Merck Group (2 mentions) Ab16048, by Abcam (2 mentions) Gapdh, by Biosynth (2 mentions) Ab16901, by Abcam (1 mentions) Ab109199, by Abcam (1 mentions) Phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions)

3 protocols using 10r g109a

1

Autophagy Induction and Analysis

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Rapamycin was purchased from Millipore. H2O2 was from Fisher Scientific. 4-hydroxytamoxifen and etoposide was from Sigma-Aldrich. The following antibodies are used: LC3 (MBL #PM036 for WB of MEFs; Cell Signaling Technology #3868 for IP, ChIP, IF, WB; Cell Signaling Technology #2775 for WB), β-tubulin (Sigma-Aldrich #T4026), calreticulin (Cell Signaling Technology #12238), COX IV (Cell Signaling Technology #4850), Atg5 (Cell Signaling Technology #8540), Atg7 (Cell Signaling Technology #8558), Lamin B1 (Abcam #ab16048), Lamin B2 (Abcam #ab8983), Lamins A/C (Millipore #MAB3211), GFP (Roche #11 814 460 001 and Abcam #ab290), p62 (Abnova #H00008878-M01), GAPDH (Fitzgerald Industries #10R-G109A), p16 (Abcam # ab16123), Ras (Millipore #05-516), HA (Sigma-Aldrich #H3663), H3K27me3 (Active Motif # 39538), H3K9me3 (Abcam #ab8898), LAMP1 (Iowa Hybridoma Bank #H4a3-s), and Flag (Sigma-Aldrich #F1804).
Dou Z., Xu C., Donahue G., Shimi T., Pan J.A., Zhu J., Ivanov A., Capell B.C., Drake A.M., Shah P.P., Catanzaro J.M., Ricketts M.D., Lamark T., Adam S.A., Marmorstein R., Zong W.X., Johansen T., Goldman R.D., Adams P.D, & Berger S.L. (2015). Autophagy mediates degradation of nuclear lamina. Nature, 527(7576), 105-109.
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2

Quantitative Protein Extraction and Analysis

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Total protein was isolated from cultured cells using RIPA buffer with added protease (Roche, 06538282001) and phosphatase inhibitors (Fisher Scientific, 502306746). Protein concentrations were quantified using a BCA assay (Pierce, PI23225) and equal amounts of protein were loaded for SDS-PAGE. GAPDH (Fitzgerald Industries International, 10R-G109a, 1:5000 dilution), p21 (Abcam, ab109199, and 1:500) and Erbb2 (Abcam, ab16901, 1:1000) primary antibodies and Anti-mouse IgG HRP-linked (Cell Signaling, 7076S) and Anti-rabbit IgG HRP linked (Cell Signaling, 7074S) secondary antibodies were used for blotting. Western blots were imaged after enhanced chemiluminescence using a Chemidoc station (Biorad).
Yücel D., Garay B.I., Perlingeiro R.C, & van Berlo J.H. (2022). Stimulation of Cardiomyocyte Proliferation Is Dependent on Species and Level of Maturation. Frontiers in Cell and Developmental Biology, 10, 806564.
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3

Autophagy Induction and Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Rapamycin was purchased from Millipore. H2O2 was from Fisher Scientific. 4-hydroxytamoxifen and etoposide was from Sigma-Aldrich. The following antibodies are used: LC3 (MBL #PM036 for WB of MEFs; Cell Signaling Technology #3868 for IP, ChIP, IF, WB; Cell Signaling Technology #2775 for WB), β-tubulin (Sigma-Aldrich #T4026), calreticulin (Cell Signaling Technology #12238), COX IV (Cell Signaling Technology #4850), Atg5 (Cell Signaling Technology #8540), Atg7 (Cell Signaling Technology #8558), Lamin B1 (Abcam #ab16048), Lamin B2 (Abcam #ab8983), Lamins A/C (Millipore #MAB3211), GFP (Roche #11 814 460 001 and Abcam #ab290), p62 (Abnova #H00008878-M01), GAPDH (Fitzgerald Industries #10R-G109A), p16 (Abcam # ab16123), Ras (Millipore #05-516), HA (Sigma-Aldrich #H3663), H3K27me3 (Active Motif # 39538), H3K9me3 (Abcam #ab8898), LAMP1 (Iowa Hybridoma Bank #H4a3-s), and Flag (Sigma-Aldrich #F1804).
Dou Z., Xu C., Donahue G., Shimi T., Pan J.A., Zhu J., Ivanov A., Capell B.C., Drake A.M., Shah P.P., Catanzaro J.M., Ricketts M.D., Lamark T., Adam S.A., Marmorstein R., Zong W.X., Johansen T., Goldman R.D., Adams P.D, & Berger S.L. (2015). Autophagy mediates degradation of nuclear lamina. Nature, 527(7576), 105-109.
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