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Xenogen ivis 100 imaging system

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The Xenogen IVIS-100 imaging system is a laboratory equipment designed for in vivo bioluminescence and fluorescence imaging. It enables non-invasive imaging of small animal models to study gene expression, cell trafficking, and disease progression.

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Lab products found in correlation

Living image software, by PerkinElmer (3 mentions) Matrigel, by BD (3 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Penicillin g, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions) Pgl3 vector, by Promega (1 mentions) Pgl3 basic vector, by Promega (1 mentions) D luciferin, by Cayman Chemical (1 mentions) Vivoglo luciferin, by Promega (1 mentions) Mab1273, by Merck Group (1 mentions)

Close spelling variants of this product

Xenogen IVIS 100 In vivo Imaging System Xenogen IVIS 100 IVIS 100 imaging system Xenogen IVIS system Xenogen IVIS IVIS 100 system IVIS imaging system IVIS 100 IVIS system

11 protocols using xenogen ivis 100 imaging system

1

Multimodal Imaging of Mouse Models

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MRI was performed on a 1 Tesla preclinical MRI scanner (M3, Aspect Imaging) with a 50 × 30 mm mouse body coil and the following parameters: Fast Spin Echo, TE/TR = 55.6 ms/6833 ms, ETL = 16, flip angle = 90°, FOV = 40 × 90 mm, matrix size = 96 × 256, 8 averages, final voxel size 0.35 × 0.35 × 0.42 mm.
BLI was performed on a Xenogen IVIS - 100 Imaging System (Perkin Elmer) as described previously15 .
Tata A., Woolman M., Ventura M., Bernards N., Ganguly M., Gribble A., Shrestha B., Bluemke E., Ginsberg H.J., Vitkin A., Zheng J, & Zarrine-Afsar A. (2016). Rapid Detection of Necrosis in Breast Cancer with Desorption Electrospray Ionization Mass Spectrometry. Scientific Reports, 6, 35374.
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2

Culturing and Transfecting Human Liver Cancer Cells

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Human HCC cell lines MHCC-97L and PLC (Japanese Cancer Research Bank; National Institutes of Biomedical Innovation, Health and Nutrition, Osaka, Japan) were maintained in high-glucose Dulbecco's modified Eagle's medium (Life Technologies Ltd.; Thermo Fisher Scientific, Inc.) supplemented with 10% heat-inactivated fetal bovine serum (Life Technologies Ltd.; Thermo Fisher Scientific, Inc.), 100 mg/ml penicillin G and 50 µg/ml streptomycin (Life Technologies Ltd., Thermo Fisher Scientific, Inc.) at 37°C in a humidified atmosphere containing 5% CO2. MHCC-97L cells were labeled via stable transfer of the luciferase gene in chromosomes. Briefly, cells were transfected with pGL3 vector (Promega Corporation, Madison, WI, USA), and positive clones were selected according to luciferase activity in the Xenogen IVIS 100 imaging system (PerkinElmer, Inc., Waltham, MA, USA).
Li C.X., Yu B., Shi L., Geng W., Lin Q.B., Ling C.C., Yang M., Ng K.T., Huang J.D, & Man K. (2016). ‘Obligate’ anaerobic Salmonella strain YB1 suppresses liver tumor growth and metastasis in nude mice. Oncology Letters, 13(1), 177-183.
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3

Carprofen and CYR5099 Modulate CFA-Induced Inflammation

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C57BL/6 male mice (7–8 weeks) were housed in an air-conditioned room with 12 h light–dark cycle, and animal studies were conducted according to the guidelines and protocol approved by Institutional Animal Care and Use Committee at Chang Gung University, Taiwan (IACUC approval No. CGU14-150). Mice were injected subcutaneously with carprofen (5 mg/kg) as analgesia and then intraperitoneally with CYR5099 (10 or 25 mg/kg body weight) or an equal volume of 0.9% saline under anesthesia (Isoflurane). After 1 h, paw inflammation was induced by intraplantar injection of CFA (1.5 mg/kg body weight in right-rear foot) or 0.9% saline (left-rear foot; as control). The change in paw thickness (nm) was determined before (baseline) and after CFA/saline injection at the indicated times. After injection of CFA for 4 h, L-012 (25 mg/kg body weight) was intraperitoneally administered. ROS production was determined using a Xenogen IVIS100 imaging system (PerkinElmer, Waltham, MA, USA) under anesthesia (30 mg/kg Zoletil 50 and 6 mg/kg xylazine) [17 (link),29 (link)]. CO2 euthanasia was used to stop the experiments. The hind paws were removed, fixed using 10% neutral buffered formalin, and embedded in paraffin blocks before cutting into 5-μm-thick sections. The staining of hematoxylin and eosin (H&E) and immunohistochemistry (Ly6G and MPO) was performed as previously described [17 (link)].
Liu F.C., Yu H.P., Chen P.J., Yang H.W., Chang S.H., Tzeng C.C., Cheng W.J., Chen Y.R., Chen Y.L, & Hwang T.L. (2019). A novel NOX2 inhibitor attenuates human neutrophil oxidative stress and ameliorates inflammatory arthritis in mice. Redox Biology, 26, 101273.
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4

Generating Luciferase-Expressing Osteosarcoma Cells

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A BamHI-EcoRI fragment of luciferase was amplified by polymerase chain reaction from the pGL3-basic vector (Promega, Fitchburg, WI, USA) and inserted into retroviral vector pBABE-puro to generate pBABE-luc-puro. Using a polyethylenimine transfection reagent, the modified pBABE-luc-puro vector was transfected together with the packaging plasmid pCL-Amphotropic into 293T cells to generate a retroviral vector supernatant. K7M2 mouse osteosarcoma cells were then infected with the retroviral vector and selected with 1 μg/mL puromycin. Single clones were obtained by limiting dilution of puromycin-resistant cells and screened under the Xenogen IVIS® 100 Imaging system (PerkinElmer, Waltham, MA, USA). Positive clones were selected and grown in duplicate to obtain K7M2–luciferase cells.
Yu Z., Geng J., Zhang M., Zhou Y., Fan Q, & Chen J. (2014). Treatment of osteosarcoma with microwave thermal ablation to induce immunogenic cell death. Oncotarget, 5(15), 6526-6539.
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5

Bioluminescent Tumor Imaging in vivo

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Live bioluminescence imaging of tumor‐associated firefly luciferase (Fluc) activity was performed using a Xenogen IVIS‐100 imaging system (PerkinElmer, Waltham, MA, USA) 3 min after intraperitoneal injection of 4.5 mg D‐luciferin in 150 μL of sterile PBS. Images were acquired using living image software (PerkinElmer).
GuhaSarkar D., Neiswender J., Su Q., Gao G, & Sena‐Esteves M. (2017). Intracranial AAV‐IFN‐β gene therapy eliminates invasive xenograft glioblastoma and improves survival in orthotopic syngeneic murine model. Molecular Oncology, 11(2), 180-193.
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6

Orthotopic Breast Cancer Tumor Progression

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For orthotopic studies, 5–6 week-old female mice were used and anesthetised before injections of 1 × 106 MDA-MB-231-LM2 cells in 50 μl of 50% Matrigel (BD Biosciences) into the fourth mammary fat pad. Four weeks following inoculation, bioluminescence imaging was performed using the Xenogen IVIS-100 imaging system (Perkin Elmer, Waltham, MA, USA). Mice were injected intraperitoneally with 30 mg/ml of D-Luciferin (in PBS, Cayman Chemical, Ann Arbor, MI, USA) 10 min before imaging. Dorsal images of the primary tumour were collected before the animals were humanely killed and their primary tumour, lung and liver harvested for ex vivo imaging. Photon emission was quantified using the Living Image Software (Perkin Elmer).
Martini C., Thompson E.J., Hyslop S.R., Cockshell M.P., Dale B.J., Ebert L.M., Woods A.E., Josefsson E.C, & Bonder C.S. (2020). Platelets disrupt vasculogenic mimicry by cancer cells. Scientific Reports, 10, 5869.
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7

In Vivo Bioluminescence Imaging of Tumor Growth

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A Xenogen-IVIS 100 imaging system (PerkinElmer, Waltham, MA, USA) was used for in vivo bioluminescence imaging (BLI). Tumor growth was monitored once per week after implantation. Mice anesthetized with isoflurane gas were intraperitoneally injected with 300 mg/kg D-luciferin (VivoGlo Luciferin; Promega, Madison, WI, USA) and placed on a warmed stage inside the camera box of the IVIS imaging system coupled with cool CCD camera using software v2.5. Images were quantified as photons per second for U87 cells, and per minute for hG008 cells.
Tamura R., Miyoshi H., Sampetrean O., Shinozaki M., Morimoto Y., Iwasawa C., Fukaya R., Mine Y., Masuda H., Maruyama T., Narita M., Saya H., Yoshida K., Okano H, & Toda M. (2019). Visualization of spatiotemporal dynamics of human glioma stem cell invasion. Molecular Brain, 12, 45.
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8

Multimodal Imaging of Tumor Resection

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Magnetic Resonance Imaging (MRI) was performed on the day of tumor resection on a 1T – M3 MR system (Aspect Imaging) with a mouse body coil 50 × 30 mm in size. Fast Spin Echo imaging was performed with the following parameters: TE/TR = 54.9 ms/4500 ms, ETL = 16, flip angle = 90°, FOV = 40 × 60 mm, matrix size = 96 × 150, 8 averages and a final pixel size of 0.4 mm.
Bioluminescence Imaging (BLI) was performed one day before tumor resection, on a Xenogen IVIS – 100 Imaging System (Perkin-Elmer). 100 μL of 25 mg mL–1d-luciferin potassium salt solution (Perkin-Elmer) was intraperitoneally administered to each mouse and images were acquired 10 minutes post-injection.
Tata A., Gribble A., Ventura M., Ganguly M., Bluemke E., Ginsberg H.J., Jaffray D.A., Ifa D.R., Vitkin A, & Zarrine-Afsar A. (2015). Wide-field tissue polarimetry allows efficient localized mass spectrometry imaging of biological tissues †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc03782d. Chemical Science, 7(3), 2162-2169.
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9

Live Bioluminescence Tumor Imaging

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Live bioluminescence imaging of tumor associated firefly luciferase (Fluc) activity was performed using a Xenogen IVIS-100 imaging system (PerkinElmer, Waltham, MA) 3 minutes after intraperitoneal injection of 4.5 mg D-luciferin in 150μl of sterile PBS. Images were acquired using Living Image software (PerkinElmer).
GuhaSarkar D., Neiswender J., Su Q., Gao G, & Sena‐Esteves M. (2017). Intracranial AAV‐IFN‐β gene therapy eliminates invasive xenograft glioblastoma and improves survival in orthotopic syngeneic murine model. Molecular Oncology, 11(2), 180-193.
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10

Xenograft Model for Retinoblastoma

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Animal protocols were in accordance with local and national guidelines. All animals were handled in agreement with the standard operating procedures of the University Health Network and all procedures were approved by the Animal Care Committee of the University Health Network, Toronto. IVT injections were performed under general anesthesia using isoflurane. Human RB cell lines were prepared at 25,000 cells/μL in sterile PBS with 10% matrigel (BD Bioscience, Mississauga, ON, Canada) and 5% trypan blue, then 2 μL of the mixture were injected into the right vitreous of 3–4 weeks-old NOD-Scid mice. Uninjected left eye served as a negative control. We used three animals per dose tested (unless specified otherwise). After seven days, MLN4924 or PBS was injected in tumor-bearing eyes at the indicated doses. Luciferase+ tumor cells were tracked live by i.p injection of d-luciferin at 150 mg/kg for 10 min and radiance total flux (photons/second) tracked with the Xenogen IVIS Imaging System 100 (PerkinElmer, Woodbridge, ON, Canada). For other RB lines, tumor volume was evaluated on sections as described44 (link). A human marker (intact mitochondria antibody, MAB1273, Millipore, Etobicoke, ON, Canada) was used to delineate tumor cells.
Aubry A., Yu T, & Bremner R. (2020). Preclinical studies reveal MLN4924 is a promising new retinoblastoma therapy. Cell Death Discovery, 6, 2.
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