Cip2a

Manufactured by Cell Signaling Technology

Sourced in United States

CIP2A is a lab equipment product offered by Cell Signaling Technology. It is a protein that functions as an important regulator of cellular processes.

Automatically generated - may contain errors

Lab products found in correlation

Cycloheximide (chx), by Merck Group (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Tunicamycin, by Merck Group (1 mentions) 4 2 aminoethyl benzenesulfonyl fluoride hydrochloride aebsf, by Merck Group (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Grp78, by Cell Signaling Technology (1 mentions) P akt ser473, by Cell Signaling Technology (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Pp2a c, by Cell Signaling Technology (1 mentions) Caspase 3, by Cell Signaling Technology (1 mentions) C myc, by Cell Signaling Technology (1 mentions) Lamin b, by Cell Signaling Technology (1 mentions) Erk inhibitor sch772984, by Selleck Chemicals (1 mentions) P c myc s62, by Cell Signaling Technology (1 mentions) Myc tag, by Cell Signaling Technology (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) β actin, by Merck Group (1 mentions)

5 protocols using cip2a

Antibody Procurement and Cell Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies against ATF6, CIP2A, GRP78, GAPDH, and β‐Actin were purchased from Cell Signaling Technology (Boston, MA, USA). Antibody against CIP2A for immunohistochemistry (IHC) was purchased from Novus Biologicals (Littleton, CO, USA), and for western blot analysis was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibody against ERK was purchased from Zymed (Grand Island, NY, USA). Tunicamycin, cycloheximide, 3‐[4, 5‐dimethylthiazol‐ 2‐y1]‐2, 5‐diphenyltetrazolium bromide) (MTT), and 4‐(2‐Aminoethyl) benzenesulfonyl fluoride hydrochloride (AEBSF) were purchased from Sigma‐Aldrich (St. Louis, MO, USA).

Liu C., Hsu C., Huang T., Lee C., Chen J., Yang S., Jiang J., Chen W., Lee K, & Teng H. (2018). ER stress‐related ATF6 upregulates CIP2A and contributes to poor prognosis of colon cancer. Molecular Oncology, 12(10), 1706-1717.

+ Open protocol

+ Expand

Evaluation of SET Antagonist TD19 Effects

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

SET antagonist TD19 (EMQA), a derivative of erlotinib with chemical name of N⁴-(3-ethynylphenyl)-6,7-dimethoxy-N²-(4-phenoxyphenyl) quinazoline-2,4-diamine), was kindly provided from Dr. Chung-Wai Shiau's lab. The chemical structure and synthesis of TD19 has been disclosed in previous studies [31 (link),33 (link)], and its mechanisms as a SET inhibitor has been also reported in prior studies [30 (link),32 (link)]. Akt inhibitor (MK-2206) inhibitor and ERK inhibitor (SCH772984) were purchased from Selleckchem (Houston, TX, USA). TD19 at different concentrations were dissolved in dimethyl sulfoxide (DMSO) and added to cells in Dulbecco's Modified Eagle Medium (DMEM) containing 1% FBS. Antibodies used for Western blots of pElk-1 and Elk were obtained from Santa Cruz Biotechnology (San Diego, CA, USA), Other such as CIP2A, PARP, PP2Ac, Akt, pAkt (Ser473), beta-actin, caspase-3, ERK, pERK, pBcl2 (Ser70), Bcl2, p c-Myc (S62), c-Myc, Lamin B, Tubulin, GFP and Myc-tag were purchased from Cell Signaling (Danvers, MA, USA). Cycloheximide (CHX) was purchased from Sigma-Aldrich (St Louis, MO, USA). MTT was purchased from Sigma-Aldrich.

Liu C.Y., Huang T.T., Chen Y.T., Chen J.L., Chu P.Y., Huang C.T., Wang W.L., Lau K.Y., Dai M.S., Shiau C.W, & Tseng L.M. (2019). Targeting SET to restore PP2A activity disrupts an oncogenic CIP2A-feedforward loop and impairs triple negative breast cancer progression. EBioMedicine, 40, 263-275.

+ Open protocol

+ Expand

Pectolinarigenin Modulates Gastric Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

The AGS and MKN28 human gastric cancer cells were obtained from the Korea Cell Line Bank (Seoul, Korea). RPMI-1640 medium, fetal bovine serum (FBS), and antibiotics (Penicillin/Streptomycin) were purchased from Gibco; Thermo Fisher Scientific, Inc. (Waltham, MA, USA). Pectolinarigenin were purchased from AdooQ (Irvine, CA, USA) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was obtained from Sigma-Aldrich. Materials and chemicals used for electrophoresis were obtained from Bio-Rad Laboratories, Inc., (Hercules, CA, USA). Antibody to LRSAM1, DDX4, PI3KCB, and CIP2A were purchased from Cell Signaling Technology (Danvers, MA, USA). β-actin was purchased from Millipore (Billerica, MA, USA). Horseradish peroxidase (HRP)—conjugated goat anti-mouse IgG (ALX-211-205TS-C100) and anti-rabbit IgG (ADI-SAB-301-J) were purchased from Enzo life sciences.

Lee H.J., Venkatarame Gowda Saralamma V., Kim S.M., Ha S.E., Vetrivel P., Kim E.H., Lee S.J., Heo J.D., Rampogu S., Lee K.W, & Kim G.S. (2018). Comparative Proteomic Profiling of Tumor-Associated Proteins in Human Gastric Cancer Cells Treated with Pectolinarigenin. Nutrients, 10(11), 1596.

+ Open protocol

+ Expand

Protein Expression Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total protein lysates were extracted from cells and equal amounts of total protein were resolved by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to a nitrocellulose membrane. Nonspecific binding was blocked with 5% milk in tris-buffered saline with Tween 20, and membranes were incubated overnight at 4°C with primary antibodies against the following proteins: CIP2A (1 : 1000), AKT1 (1 : 2000), phosphorylated AKT1 (p-AKT1) (Ser473) (1 : 1500), PI3K p85 (1 : 500), p-PI3K p85 (Tyr458) (1 : 1000), mTOR (1 : 1000), p-mTOR (Ser2448) (1 : 2000), and poly(ADP-ribose) polymerase (PARP, 1 : 1500) (all from Cell Signaling Technology, Danvers, MA, USA). Membranes were then incubated with horseradish peroxidase-conjugated secondary antibody and protein bands were detected by enhanced chemiluminescence (Thermo Scientific, Waltham, MA, USA).

Yang X., Zhang Y., Liu H, & Lin Z. (2016). Cancerous Inhibitor of PP2A Silencing Inhibits Proliferation and Promotes Apoptosis in Human Multiple Myeloma Cells. BioMed Research International, 2016, 6864135.

+ Open protocol

+ Expand

Targeted Gene Silencing by siRNAs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gene silencing was performed by siRNAs transfection according to Nucleofector technology (Lonza): mTOR, PP1, PP2A, CIP2A and non-targeting control siRNAs were from Cell Signaling Technologies (Danvers, MA, US).

Bartalucci N., Calabresi L., Balliu M., Martinelli S., Rossi M.C., Villeval J.L., Annunziato F., Guglielmelli P, & Vannucchi A.M. (2017). Inhibitors of the PI3K/mTOR pathway prevent STAT5 phosphorylation in JAK2V617F mutated cells through PP2A/CIP2A axis. Oncotarget, 8(57), 96710-96724.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!