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3 protocols using bms 5

1

Luteolin and BMS-5 Cellular Assays

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Luteolin (purity ≥ 98%) was purchased from Sichuan Weikeqi Biological Technology CO., Ltd (CAS#: 491‐70‐3). BMS‐5 (purity ≥ 98%) was purchased from Enzo Life Sciences (CAS#:1338247‐35‐0). CNBr‐activated SepharoseTM 4B (Lot# 10265330) was purchased from GE Healthcare. Thiazolyl blue tetrazolium bromide (MTT) powder was purchased from Solarbio Technology Co., Ltd. Dimethylsulfoxide (DMSO) was purchased from Sigma. Cyclin D1 (catalog# 2922), cyclin D3 (catalog# 2936), Bax (catalog# 5023), cleaved‐PARP (catalog# 5625), caspase‐3 (catalog# 9662), cleaved caspase‐3 (catalog# 9664), caspase‐7 (catalog# 9492), cleaved caspase‐7 (catalog# 8438), ROCK1 (catalog# 4035) and ROCK2 (catalog# 9029) were purchased from Cell Signaling Technology. Phospho‐LIMK‐1/2 (Thr 508/505)‐R (catalog# sc‐28409‐R), LIMK‐1(catalog# sc‐28370), cofilin (catalog# sc‐33779) and phospho‐cofilin (mSer3)‐R (catalog# sc‐21867‐R) were purchased from Santa Cruz Technology.
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2

LXA4 Potentiation by LIMK Inhibition

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In select experiments, NK cells were exposed to the LIMK inhibitor BMS-5 (Enzo Life Sciences) at 1 μM, 5 μM, or 10 μM for 48 hours. Vehicle was 0.02% DMSO. In indicated experiments, NK cells were exposed to BMS-5 10 μM for 15 minutes prior to LXA4 (100 nM). BMS-5 and LXA4 were re-dosed at 24 hours.
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3

Analyzing Dasatinib-Induced Apoptosis

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Dasatinib (purity ≥ 99%) was purchased from Santa Cruz Biotechnology (CAS: 302962-49-8, Lot# H0515) (Beverly, MA, United States). BMS-5 (purity ≥ 98%) was bought from Enzo Life Sciences (CAS:1338247-35-0) (Shanghai, China). Dimethylsulfoxide (DMSO) and CNBr-activated SepharoseTM 4B (Lot# 10265330) were purchased from Sigma (St. Louis, MO, United States) and GE Healthcare (Uppsala, Sweden) respectively. Cyclin D1 (Cat# 2922), cyclin D3 (Cat# 2936), cleaved-PARP (Cat# 5625), caspase-3 (Cat# 9662), cleaved caspase-3 (Cat# 9664) caspase-7 (Cat# 9492), and cleaved caspase-7 (Cat# 8438) were purchased from Cell Signaling Technology (Beverly, MA, United States). p-LIMK (Thr 508/505)-R (Catalog# sc-28409-R), LIMK-1 (Catalog# sc-28370), Cofilin (Catalog# sc-33779), p-Cofilin (mSer3)-R (Catalog# sc-21867-R) were purchased from Santa Cruz Technology. β-actin antibody (Cat# TA-09) as a loading control was obtained from ZSGB-Bio Company (Beijing, China).
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