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Custom oligonucleotides

Manufactured by Merck Group
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Custom oligonucleotides are synthetic DNA or RNA molecules of specific sequences, designed and produced to order. They are used as tools in various life science applications, such as genetic research, diagnostics, and therapeutics development.

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Lab products found in correlation

Primestar max dna polymerase, by Takara Bio (1 mentions) Nebuilder hifi dna assembly master mix, by New England Biolabs (1 mentions) Pfu turbo dna polymerase, by Agilent Technologies (1 mentions) Phusion high fidelity dna polymerase, by Thermo Fisher Scientific (1 mentions) Taq dna polymerase, by New England Biolabs (1 mentions) Standard taq buffer, by New England Biolabs (1 mentions) Pcmv gluc, by New England Biolabs (1 mentions)

Close spelling variants of this product

Custom-synthesized oligonucleotide primers Custom DNA oligonucleotides Oligonucleotides

4 protocols using custom oligonucleotides

1

Optimized DNA Assembly Protocol

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All reagents were purchased from Fisher Scientific®, unless stated otherwise. Custom oligonucleotides were purchased from Merck Sigma Aldrich. PrimeStar® Max DNA polymerase was purchased from TaKaRa. Restriction enzymes were purchased from ThermoFisher Scientific. NEBuilder® HiFi DNA Assembly Master Mix was purchased from New England Biolabs.
Hayes H.C, & Luk L.Y. (2023). Investigating the effects of cyclic topology on the performance of a plastic degrading enzyme for polyethylene terephthalate degradation. Scientific Reports, 13, 1267.
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2

Molecular Cloning Protocol Essentials

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PCR fragments used for strain and plasmid constructions were amplified with the Phusion high fidelity DNA polymerase (Thermo scientific). Colony PCR amplifications were performed with Taq DNA Polymerase with Standard Taq Buffer (New England BioLabs). Site-directed Quickchange mutagenesis amplifications were done using the Pfu Turbo DNA polymerase (Agilent Technologies). Restriction enzymes were purchased from New England BioLabs. Custom oligonucleotides (listed in S2 Table) were synthesized by Sigma Aldrich.
Gueguen E., Wills N.M., Atkins J.F, & Cascales E. (2014). Transcriptional Frameshifting Rescues Citrobacter rodentium Type VI Secretion by the Production of Two Length Variants from the Prematurely Interrupted tssM Gene. PLoS Genetics, 10(12), e1004869.
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3

Generation and Modification of TA-Luciferase Lentiviral Vectors

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An HIV-based transfer vector encoding CMV-GFP44 (link) was modified to encode TA-FLuc (plenti-TA-FLuc) by exchanging the CMV-GFP cassette with TA-FLuc from the Panomics translucent control vector (Panomics, Madison, WI) using NheI and XbaI restriction enzymes. This construct was further modified to create a library of lentivirus-producing transfer vector constructs with TF-responsive binding elements. Sequences derived from Panomics constructs were digested out of the constructs also using NheI and XbaI and ligated into the plenti-TA-FLuc backbone. For non-Panomics constructs, such as TCF/LEF45 (link), CMYC14 (link), NOTCH146 (link), and PTTG47 (link), custom oligonucleotides were synthesized (Sigma Aldrich), annealed, and inserted into the plenti-TA-FLuc backbone using NheI and BglII. A vector encoding Gaussia luciferase (GLuc), TA-GLuc, was also constructed by transferring the GLuc gene from pCMV-GLuc (New England Biolabs, Ipswich, MA) into the Panomics vector using HindIII and XbaI, and subsequently following the same procedure as the other Panomics-derived constructs.
Weiss M.S., Bernabé B.P., Shin S., Asztalos S., Dubbury S.J., Mui M.D., Bellis A.D., Bluver D., Tonetti D.A., Saez-Rodriguez J., Broadbelt L.J., Jeruss J.S, & Shea L.D. (2014). Dynamic transcription factor activity and networks during ErbB2 breast oncogenesis and targeted therapy. Integrative biology : quantitative biosciences from nano to macro, 6(12), 1170-1182.
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4

Oligonucleotide-Based Protocol Details

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Custom oligonucleotides were from Sigma-Aldrich. Sources of chemicals and method details are in the Supporting Information file.
Jiang D., Malla S., Fu Y.J., Choudhary D, & Rusling J.F. (2017). Direct LC-MS/MS Detection of Guanine Oxidations in Exon 7 of the p53 Tumor Suppressor Gene. Analytical chemistry, 89(23), 12872-12879.
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