J2 HIE monolayers were incubated with VLPs for 1 h at 37 oC. The cells were washed twice with PBS and VLP-treated and untreated control cells were trypsinized and fixed in 2% paraformaldehyde–3% phosphate-buffered glutaraldehyde. Thin sections were prepared and electron microscopy was carried out using a FEI Tecnai Spirit transmission electron microscope equipped with a 4 K Eagle digital camera73 (link).
4k eagle digital camera
Manufactured by Thermo Fisher Scientific
Sourced in Japan, United States
The 4K Eagle digital camera is a high-resolution imaging device designed for scientific and industrial applications. It captures images and video at 4K resolution (3840 x 2160 pixels) with high-quality optics and advanced sensor technology. The camera provides precise, detailed visual data for a variety of research and analysis needs.
Automatically generated - may contain errors
Lab products found in correlation
Durcupan resin, by Merck Group (2 mentions)
Tecnai g2 spirit biotwin, by Thermo Fisher Scientific (2 mentions)
Jem 1200 transmission electron microscope, by JEOL (1 mentions)
Tecnai g2 transmission electron microscope, by Thermo Fisher Scientific (1 mentions)
Tecnai tf20, by Thermo Fisher Scientific (1 mentions)
6 protocols using 4k eagle digital camera
1
Viral Uptake in J2 HIE Monolayers
2
Ultrastructural Analysis of S. aureus USA300 Treated with ChryA
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The effects of ChryA on the cell structure of S. aureus USA300 were examined with a transmission electron microscope. Briefly, S. aureus USA300 cells in the logarithmic phase were treated with 0.5× MIC of ChryA and fosfomycin (MIC = 4 μg/ml) at 37°C in fresh LB medium. DMSO was used as a negative control. Samples were centrifuged at 2000g for 10 min, and bacterial pellets were fixed by resuspending in 2% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4). Bacterial pellets were then embedded in 2% agarose and postfixed with 1% osmium tetroxide overnight at room temperature. After washing, samples were dehydrated multiple times in increasing concentrations of ethanol and embedded in Durcupan resin (Sigma-Aldrich). Fifty-five–nanometer sections were examined using a JEM-1200 transmission electron microscope (JEOL; Akishima, Tokyo, Japan) equipped with a 4-K Eagle digital camera (FEI, Hillsboro, OR, USA).
3
Isolation and Visualization of Triglyceride-Rich Lipoproteins in Zebrafish
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Pooled plasma from fasted overnight WT and apoc2 mutant zebrafish was diluted in PBS, without density adjustment, and the samples were centrifuged at 200,000 g for 4 h at 4°C. The top layer containing TRLs was collected, supplemented with 0.1% sucrose, added to a FCF100-Cu 100 mesh (Electron Microscopy Sciences, Hatfield, PA) and negatively stained with 1% uranyl acetate. Stained samples were imaged with a Tecnai G2 Spirit BioTWIN transmission electron microscope equipped with a 4K Eagle digital camera (FEI Company, Hillsboro, OR).
4
TEM Analysis of H. pylori Structural Changes by LipoLLA and LipoOA
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The effects of LipoLLA and LipoOA on the structure of H. pylori were examined with TEM. Briefly, an overnight culture of H. pylori was treated with LipoLLA or LipoOA (final concentration 400 μg/mL) and incubated for 5 or 30 min. In addition, H. pylori were treated with 200 μg/mL of LipoLLA for 30 min. PBS was used as negative control. Samples were centrifuged at 3000 × g for 5 min and bacterial pellets fixed by resuspending in 2% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.4). Bacterial pellets were then embedded in 2% agarose, post-fixed with 1% osmium tetroxide, and processed in Durcupan resin (Sigma-Aldrich). Fifty-five nm sections were examined using a FEI Tecnai G2 transmission electron microscope equipped with a 4K Eagle digital camera (FEI, Hilsboro, OR).
5
Transmission Electron Microscopy of Samples
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Samples were adhered to 100 mesh Formvar and carbon coated grids for 10 min at room temperature. Grids were washed 3 × 1 min with deionized water, stained with 2% uranyl acetate (Ladd Research Industries, Williston VT) in water for 1 minute, dried and viewed using a Tecnai G2 Spirit BioTWIN transmission electron microscope equipped with an Eagle 4k digital camera (FEI, Hilsboro, OR, USA).
6
Transmission Electron Microscopy Protocol
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