Nod scid il2rgammanull nsg

BALB/c and NOD-scid-IL2R gamma^null (NSG) mice were obtained from the Jackson Laboratory. Animals were housed under pathogen-free conditions according to the guidelines of the Division of Comparative Medicine, Washington University, St. Louis, MO. All animal experiments were approved by the Washington University Animal Studies Committee.
For xenograft experiments, 6–8 week old female NSG mice were inoculated with 5×10⁵ MDA-MB-231 cells in Matrigel (BD Biosciences) in the #9 mammary fat pad to generate orthotopic breast tumors. As an experimental model of bone metastasis, 1×10⁵ 4T1 or MDA-MB-231 cells were injected into the left cardiac ventricle as previously described (40 (link)).
In neoadjuvant-adjuvant regimens, POL5551 was administered at the dose of 20 mg/kg subcutaneously twice a day from day 7 as a monotherapy, or from day 10 in combination with eribulin. Eribulin was administered on day 10, 17 and 24 (0.1 mg/kg, i.v.). For the “framing dosing” experiment, on day 10 POL5551 (20 mg/kg) was administered subcutaneously 4 hours before, 4 hours after, and again 18 hours after chemotherapy with erubulin (0.2 mg/kg, i.v.). A simulated exposure profile following administration of POL5551 using this dose regimen is shown in Supplemental Figure 1C. Vehicle-treated controls received saline solution (i.v. or s.c. as appropriate, see individual experiments for details).

NOD-Scid IL-2Rgammanull (NSG) and pmel mice were obtained from the Jackson Laboratory, and C57BL/6 mice were purchased from Charles River Laboratories. All experiments have been reviewed and approved by the Institutional Animal Care and Use Committee. HLA-A2+/MART-1+ Malme-3M cells were maintained in Iscove's modified Dulbecco's medium, and HLA-A2+/MART-1+ C32, HLA-A2+/MART-1- A375, B16, Phoenix Ampho and Eco packaging cell lines in Dulbecco's modified Eagle's medium, supplemented with fetal bovine serum and penicillin-streptomycin.

Aldh1L1^−GFP mice were provided by Dr. Jeffery Rothstein (John’s Hopkins University School of Medicine); Aldh1L1-CreER^T2 mice were provided by Dr. Baljit Khakh (University of California, Los Angeles); Math1^−GFP, Sox2^−GFP, Sox2-CreER^T2, Sox2^−loxp, Rosa-CAG-LSL-tdTomato, NOD-SCID-IL2RGamma null (NSG) and C57BL/6J mice were purchased from the Jackson Laboratory. Mice were maintained in the Animal Facility at Children’s National Health System (CNHS). All experiments were performed in accordance with national guidelines and regulations, and with the approval of the animal care and use committee at CNHS.