Dichloromethane

Glycinamide hydrochloride and serine methyl ester hydrochloride were purchased from Watanabe Chemical Industry Co, Ltd. (Hiroshima, Japan). Acryloyl chloride, hydrochloric acid (12 M), potassium carbonate, sodium hydroxide, diethyl ether, methanol, ethanol, tetramethylsilane (TMS), ammonium persulfate (APS), sodium trimethylsilyl propane sulfonate (DSS), N,N-diisopropylethylamine (DIPEA), dichloromethane (DCM), and anhydrous sodium sulfate (Na₂SO₄) were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Deuterium oxide (D₂O), 25% glutaraldehyde solution, and Wako Gel^® FC-40 were purchased from FUJIFILM Wako Pure Chemical, Co. (Osaka, Japan). Deuterated dimethyl sulfoxide (DMSO-d₆) was purchased from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA). Polyethylene glycol diacrylate (PEGDA) was purchased from Tokyo Chemical Industry Co. (Tokyo, Japan) and dialysis membrane (MWCO: 25,000 Da) from Funakoshi Co. (Tokyo, Japan). The glass plates (76 mm × 52 mm) were obtained from Matsunami Glass Industry Co., Ltd. (Osaka, Japan). glass plates were hydrophobically treated with Siliconize L-25 (Fuji Systems Co., Tokyo, Japan) before use. Silicone rubber plates (1 mm) were obtained from As One Co. (Osaka, Japan). All the reagents were used without further purification.

α-N-Hydroxysuccinimidyl-ω-Boc protected-amino-PEG (NHS-PEG-NH₂-Boc, Mw: 5000), 1,2-dimyristoyl-sn-glycerol-3-phosphatidylethanolamine (DMPE), 1,2-dipalmitoyl-sn-glycerol-3-phosphatidylethanolamine (DPPE), and 1,2-distearoyl-sn-glycerol-3-phosphatidylethanolamine (DSPE) were purchased from NOF Corporation (Tokyo, Japan). Dichloromethane, chloroform, toluene, diethyl ether, and n-butyl amine were obtained from Nacalai Tesque (Kyoto, Japan). Methoxyl-PEG-succinimidyl propionate (MeO-PEG-NHS, Mw 5000) was purchased from Nektar Therapeutics (San Carlos, CA, USA). L-α-Phosphatidylcholine from egg yolk (EggPC) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Fluorescein isothiocyanate (FITC) was obtained from Dojindo Laboratories (Kumamoto, Japan). Phosphate-buffered saline (PBS) was obtained from Nissui Pharmaceutical, Co., Ltd (Tokyo, Japan). 1-Hexadecanethiol was purchased from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan). Trifluoroacetic acid (TFA) and phospholipid C-test wako were purchased from Wako Pure Chemical Industries, Ltd (Osaka, Japan). Octadecyl triethoxysilane was supplied from Shin-Etsu Chemical Co., Ltd (Tokyo, Japan). L-α-Palmitoyl-NBD dodecanoyl phosphatidylethanolamine was purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA).