Dogs were anaesthetized using butorphanol (0.2 mg/kg; Butomidor®, Richter Pharma, silures, Autria) in combination with medetomidine (5 μg/kg; Medetor®, CP-Pharma, Burgdorf, Germany) intravenously. Propofol (2–4 mg/kg to effect; Propovet®, Zoetis, Malakoff, France) intravenously was used for induction. Anesthesia was maintained with isoflurane (Iso-Vet®; Eurovet, Bladel, Netherlands). Dogs with intra-tracheal tubes were placed in ventral recumbency; the nasopharynx was manually obstructed. A 4-cm-long 12-Fr fenestrated catheter connected to a 60-mL syringe was introduced in the first third of the left nasal cavity. Twenty mL (20 ± 1 mL/kg) of sterile isotonic saline solution was then injected, with the ipsilateral nostril being completely manually obstructed to prevent leakage of the solution. The fluid was then directly aspirated in the syringe using manual suction while progressively removing the catheter from the nasal cavity (Video S1 ). The time of the procedure was recorded. Venous blood samples were obtained after the NALF procedure by jugular venipuncture using 5-mL plastic syringes and 21-G needles and placed immediately into 5-mL dry tubes.
Medetor
Manufactured by CP-Pharma
Sourced in Germany
Medetor is a precision laboratory instrument used for the accurate measurement and analysis of various samples. It is designed to provide consistent and reliable data for scientific research and testing purposes.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using medetor
1
Nasal Lavage Fluid Collection in Dogs
2
Canine Nasal Microbiome Sampling Protocol
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This study was approved by the animal ethical committee (N° 16–1854, 27/10/2016) and all samples were obtained with the consent of the owners.
For sample collection, dogs were premedicated with a combination of butorphanol (Butomidor®, Richter Pharma) and medetomidine (Medetor®, CP-Pharma) intravenously. Propofol (Propovet®, Zoetis) on demand was used for induction. Under general anesthesia, to prevent sample contamination, a sterile speculum was inserted into the nare to allow the passage of a sterile swab (Copan®, FLOQSwabs™, 553C, Brescia, Italy) in the distal third of the nasal cavity. Sample collection was performed either by EV, FB or CC. In case of unilateral fungal rhinitis, the affected nasal cavity was sampled. In diseased dogs, sample collection was performed before rhinoscopy. The nasal mucosa was brushed using three careful circular movements before withdrawal of the swab through the speculum. The tip of the saw was cut and stored in a sterile cryotube and banked at -80 °C until further analyses.
For sample collection, dogs were premedicated with a combination of butorphanol (Butomidor®, Richter Pharma) and medetomidine (Medetor®, CP-Pharma) intravenously. Propofol (Propovet®, Zoetis) on demand was used for induction. Under general anesthesia, to prevent sample contamination, a sterile speculum was inserted into the nare to allow the passage of a sterile swab (Copan®, FLOQSwabs™, 553C, Brescia, Italy) in the distal third of the nasal cavity. Sample collection was performed either by EV, FB or CC. In case of unilateral fungal rhinitis, the affected nasal cavity was sampled. In diseased dogs, sample collection was performed before rhinoscopy. The nasal mucosa was brushed using three careful circular movements before withdrawal of the swab through the speculum. The tip of the saw was cut and stored in a sterile cryotube and banked at -80 °C until further analyses.
3
Nasal Microbiome Sampling in Dogs
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Nasal swabs were taken under general anesthesia in all dogs, and before performing rhinoscopy in dogs with nasal disease. In the cured SNA group, nasal swabs were performed 30 seconds before to the recheck rhinoscopy (which was 3‐4 weeks after endoscopic debridement or surgical management). All dogs were premedicated with butorphanol (0.2 mg/kg; Butomidor, Richter Pharma) IV in combination with medetomidine (5 μg/kg; Medetor, CP‐Pharma). Propofol (2‐4 mg/kg; Propovet, Zoetis) was used for induction. Anesthesia was maintained with isoflurane (Iso‐Vet; Eurovet).
A nasal smear was taken either from the left (in healthy dogs) or the most affected nasal cavity based on history and clinical exam. Samples were taken under general anesthesia in intubated dogs. For this purpose, the nare was maintained open using a sterile speculum and a sterile swab (Copan, FLOQSwabsTM, 553C, Brescia, Italy) was introduced blindly through the speculum up to approximately the caudal third of the nasal cavity. Three gentle complete circular movements were used to brush the mucosa before withdrawal of the swab through the speculum. The swab tip was then cut and stored in a sterile cryotube and banked at −80°C until batched analyses to extract DNA. In SNA and Non‐SNA nasal disease groups, the procedure was before rhinoscopy.
A nasal smear was taken either from the left (in healthy dogs) or the most affected nasal cavity based on history and clinical exam. Samples were taken under general anesthesia in intubated dogs. For this purpose, the nare was maintained open using a sterile speculum and a sterile swab (Copan, FLOQSwabsTM, 553C, Brescia, Italy) was introduced blindly through the speculum up to approximately the caudal third of the nasal cavity. Three gentle complete circular movements were used to brush the mucosa before withdrawal of the swab through the speculum. The swab tip was then cut and stored in a sterile cryotube and banked at −80°C until batched analyses to extract DNA. In SNA and Non‐SNA nasal disease groups, the procedure was before rhinoscopy.
4
Nasal Swab Collection from Anesthetized Dogs
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This study was approved by the ethical committee of the University of Liege (approval number: 1854) and all samples were obtained with informed and written owner consent.
Dogs were premedicated with butorphanol (0.2 mg/kg; Butomidor®, Richter Pharma) intravenously in combination with medetomidine (5 μg/kg; Medetor®, CP-Pharma). Propofol (Propovet®, Zoetis) on demand was used for induction without intubation. Under general anesthesia the nostril was maintained open using a sterile speculum, and a sterile swab (Copan®, FLOQSwabs™, 553C, Brescia, Italy) was introduced through the speculum up to the distal third of the right nasal cavity (Fig.7 ). Three gentle complete circular movements were used to brush the mucosa before withdrawal of the swab through the speculum. The top of the swab was then cut, stored in a sterile cryotube and banked at − 80 °C until further analyses.![]()
Dogs were premedicated with butorphanol (0.2 mg/kg; Butomidor®, Richter Pharma) intravenously in combination with medetomidine (5 μg/kg; Medetor®, CP-Pharma). Propofol (Propovet®, Zoetis) on demand was used for induction without intubation. Under general anesthesia the nostril was maintained open using a sterile speculum, and a sterile swab (Copan®, FLOQSwabs™, 553C, Brescia, Italy) was introduced through the speculum up to the distal third of the right nasal cavity (Fig.
Sample collection. A sterile swab (Copan®, FLOQSwabs™, 553C, Brescia, Italy) was introduced through a sterile speculum up to the distal third (dashed line) of the nasal cavity. Three complete circular movements were used to brush the nasal mucosa before withdrawal of the swab through the speculum
5
Nasal Swab Sampling in Dogs
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This study was approved by the ethical committee of the University of Liege (approval number: 1854) and all samples were obtained with informed and written owner consent.
Dogs were premedicated with butorphanol (0.2 mg/kg; Butomidor®, Richter Pharma) intravenously in combination with medetomidine (5 g/kg; Medetor®, CP-Pharma). Propofol (Propovet®, Zoetis) on demand was used for induction without intubation. Under general anesthesia the nostril was maintained open using a sterile speculum, and a sterile swab (Copan , FLOQSwabs TM , 553C, Brescia, Italy) was introduced through the speculum up to the distal third of the right nasal cavity (Figure 1). Three gentle complete circular movements were used to brush the mucosa before withdrawal of the swab through the speculum. The top of the swab was then cut, stored in a sterile cryotube and banked at -80°C until further analyses.
Dogs were premedicated with butorphanol (0.2 mg/kg; Butomidor®, Richter Pharma) intravenously in combination with medetomidine (5 g/kg; Medetor®, CP-Pharma). Propofol (Propovet®, Zoetis) on demand was used for induction without intubation. Under general anesthesia the nostril was maintained open using a sterile speculum, and a sterile swab (Copan , FLOQSwabs TM , 553C, Brescia, Italy) was introduced through the speculum up to the distal third of the right nasal cavity (Figure 1). Three gentle complete circular movements were used to brush the mucosa before withdrawal of the swab through the speculum. The top of the swab was then cut, stored in a sterile cryotube and banked at -80°C until further analyses.
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