Total protein was extracted, separated and transferred to membranes, which were then blocked with 5% skim milk. Next, the protein‐loaded membrane was probed with antibodies against APE1 (ab92744, 1:500, Abcam), MDR1 (ab170904, 1:500), MRP (ab261871, 1:500), LRP (ab273093, 1:500), ABCG2 (ab207732, 1:500), STAT3 (ab68153, 1:500, Abcam), p‐STAT3 (ab267373, 1:1000, Abcam), Bcl‐2 (ab196495, 1:1000, Abcam), Bax (ab53154, 1:500, Abcam), cleaved caspase‐3 (ab2302, 1:1000, Abcam) and β‐actin (ab8227, 1:500, Abcam) and then with HRP‐labelled anti‐rabbit IgG secondary antibodies (No. 7074, 1:5000; CST, Danvers, MA) at 37°C for 1 h. The blots were visualized via an ECL reagent (32106, Thermo Fisher Scientific), normalized to β‐actin.
Ab92744
Manufactured by Abcam
Sourced in United States
Ab92744 is a primary antibody product offered by Abcam. It is a polyclonal antibody that recognizes a specific target protein. The core function of this product is to bind to and detect the target protein in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Ecl reagent, by Thermo Fisher Scientific (1 mentions)
Ab53154, by Abcam (1 mentions)
Ab207732, by Abcam (1 mentions)
Ab68153, by Abcam (1 mentions)
Ab196495, by Abcam (1 mentions)
Ab267373, by Abcam (1 mentions)
Ab170904, by Abcam (1 mentions)
Ab273093, by Abcam (1 mentions)
Ab8227, by Abcam (1 mentions)
Ab2302, by Abcam (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
2 protocols using ab92744
1
Protein Expression Profiling Protocol
2
Western Blot Analysis of APE1 Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Samples (15 µg protein) were separated by 12% SDS-PAGE and were transferred to polyvinylidene difluoride membranes (EMD Millipore). The membranes were then blocked with 5% skim milk for 1 h at room temperature, and were incubated with APE1 antibody (ab92744; dilution, 1:20,000; Abcam, Cambridge, MA, USA) overnight at 4°C. The membranes were washed with Tris-buffered saline containing 0.05% Tween and were then incubated with horseradish peroxidase-conjugated secondary antibody (cat. no. 129736; dilution, 1:10,000; ZSGB-BIO Co., Ltd, Beijing, China) for 1 h at room temperature. Finally the blots were visualized using chemiluminescence (EMD Millipore). β-actin (#4790; dilution, 1:10,000; Cell Signaling Technology, Inc.) was used as a loading control. Semi-quantitative analysis of the blots was performed using ImageJ2x (National Institutes of Health, Bethesda, MD, USA). Protein concentration was determined using the bicinchoninic acid method prior to western blotting.
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