Sodium 3 mercapto 1 propanesulfonate

Manufactured by Merck Group

Sodium 3-mercapto-1-propanesulfonate is a chemical compound used in laboratory settings. It is a source of the sulfhydryl (thiol) functional group. The compound serves as a reagent for various analytical and synthetic applications.

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Lab products found in correlation

Poly allylamine hydrochloride, by Merck Group (2 mentions) 1 2 dioleoyl sn glycero 3 phospho l serine, by Avanti Polar Lipids (2 mentions) Valinomycin, by Thermo Fisher Scientific (1 mentions) Sodium borohydride, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Melamine, by Merck Group (1 mentions) Hydrogen tetrachloroaurate trihydrate, by Merck Group (1 mentions) Poly styrenesulfonate sodium salt, by Merck Group (1 mentions) Chloroform, by Merck Group (1 mentions) 1 2 dioleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Gramicidin, by Merck Group (1 mentions)

4 protocols using sodium 3 mercapto 1 propanesulfonate

Functionalization of Pt5 Nanoparticles

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Immediately after the synthesis
of the citrate-capped Pt5 NPs,^{27 (link)} 0.8 mL
of a solution 50 mM sodium 3-mercapto-1-propanesulfonate (Sigma-Aldrich),
and 2 mL of a solution 20 mM of 3-mercaptopropionic acid, MPA (Sigma-Aldrich),
were sequentially added to the reaction mixture. The solution was
kept under vigorous stirring for at least 1 h at 80 °C. Thiol
molecules form a self-assembled monolayer on the citrate-capped Pt5
NPs due to the high affinity of thiols for noble metals^{51 (link)−56 (link)} and the strong Pt–sulfur interaction.^{57 (link),58 (link)} Afterward, the solution was cooled to room temperature, washed with
Milli-Q water using 50 K Amicon Ultra centrifugal filters, and stored
under 4 °C for future experiments. Pt5 NPs were then characterized
by dynamic light scattering (DLS) spectroscopy using a Zetasizer Nano
ZS System equipped with a 633 nm He–Ne laser (Malvern Instruments).

De Luca E., Pedone D., Moglianetti M., Pulcini D., Perrelli A., Retta S.F, & Pompa P.P. (2018). Multifunctional Platinum@BSA–Rapamycin Nanocarriers for the Combinatorial Therapy of Cerebral Cavernous Malformation. ACS Omega, 3(11), 15389-15398.

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Biomimetic Membrane Formation Protocol

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The following chemicals were purchased from Sigma-Aldrich: Poly(allylamine hydrochloride) (PAH, Mw 15 kDa), poly(styrenesulfonate sodium salt) (PSS, Mw 70 kDa), sodium 3-mercapto-1-propanesulfonate (MPS, Mw 178.21 g/mol), Tris buffer, consisting of 10 mM Tris adjusted to pH 7.4 by titration with HCl, phosphate-buffered saline (PBS), potassium chloride (KCl), sodium chloride (NaCl), calcium chloride (CaCl2), magnesium chloride (MgCl2) and chloroform (anhydrous, >99%). Valinomycin was purchased from Life Techn (Thermo Fisher). Phospholipids 1,2-dioleoyl-snglycero-3-phosphocholine (DOPC, 25 mg mL -1 in chloroform) and 1,2-dioleoyl-snglycero-3-phospho-L-serine (DOPS, sodium salt, 25 mg mL -1 in chloroform) were obtained from Avanti Polar Lipids Inc., Ethanol (99.9% HPLC) was obtained from Scharlau S.A. All aqueous solutions were prepared with Millipore water (resistivity of 18 MΩ*cm). All solutions were degassed with nitrogen before use.

Gutiérrez-Pineda E., Andreozzi P., Diamanti E., Anguiano R., Ziolo R.F., Moya S.E., José Rodríguez-Presa M, & Gervasi C.A. (2021). Effects of valinomycin doping on the electrical and structural properties of planar lipid bilayers supported on polyelectrolyte multilayers. Bioelectrochemistry (Amsterdam, Netherlands), 138.

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Synthesis of Gold Nanoparticles

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Hydrogen tetrachloroaurate trihydrate (HAuCl4•3H2O, 99.0%, P.M. 393.83 g/mol); sodium 3mercapto-1-propanesulfonate (HS(CH2)3SO3Na, 3MPS, 90.0%, P.M. 178.21 g/mol); sodium borohydride (NaBH4); melamine (C3H6N6, 1,3,5-Triazine-2,4,6-triamine, 99%, P.M. 126.12 g/mol); hydrochloric acid (HCl, ≥37%) were purchased from Sigma Aldrich and used as received. Milli-Q water (18.3 MΩ•cm) was used in all experiments. All glassware was cleaned by freshly prepared aqua regia (HCl 37%/HNO3≥65% 3:1 by volume), rinsed thoroughly with deionized water and stored at 180°C before use.

Cerra S., Salamone T.A., Sciubba F., Marsotto M., Battocchio C., Nappini S., Scaramuzzo F.A., Li Voti R., Sibilia C., Matassa R., Beltrán A.M., Familiari G, & Fratoddi I. (2021). Study of the interaction mechanism between hydrophilic thiol capped gold nanoparticles and melamine in aqueous medium. Colloids and surfaces. B, Biointerfaces, 203.

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Cationic-Anionic Lipid Bilayer Formation

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Poly(allylamine hydrochloride) (PAH, M w 15 kDa), poly-(styrenesulfonate sodium salt) (PSS, M w 70 kDa), sodium 3-mercapto-1propanesulfonate (MPS, M w 178.21 g mol À1 ), gramicidin (A, B and C mixture) from Bacillus aneurinolyticus (Bacillus brevis), phosphate-buffered saline (PBS), potassium chloride (KCl), sodium chloride (NaCl), calcium chloride (CaCl 2 ) and chloroform (anhydrous, 499%) were purchased from Sigma-Aldrich. The phospholipids 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC, 10 mg mL À1 in chloroform) and 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS, sodium salt, 10 mg mL À1 in chloroform) were obtained from Avanti Polar Lipids, Inc. Ethanol (99.9% HPLC) was obtained from Scharlau S.A.

Diamanti E., Gutiérrez-Pineda E., Politakos N., Andreozzi P., Rodriguez-Presa M.J., Knoll W., Azzaroni O., Gervasi C.A, & Moya S.E. (2017). Gramicidin ion channels in a lipid bilayer supported on polyelectrolyte multilayer films: an electrochemical impedance study. Soft matter, 13(47).

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