Met d1c2 xp

MHCC97-H cells were grown on four-well chamber slides and processed using the Duolink In Situ Fluorescence kit with red detection reagents (Sigma-Aldrich) per the manufacturer’s instructions. MET (D1C2 XP– Cell Signaling) and HER3 G4 (SC-203) primary antibodies were used.

Total cell lysates were separated by SDS-PAGE and transferred to activated PVDF membranes using the TransBlot Turbo system (BioRad). Membranes were blocked and probed with primary and secondary antibodies according to standard techniques. Images were acquired using an Odyssey FC Imager (LI-COR) and quantified using Image J. Immunoblotting was performed with primary antibodies against actin (Sigma, A5441), pMET(T1234/1235) (D26)XP (Cell Signaling, #3077), MET (D1C2)XP (Cell Signaling #8198), p44/42MAPK(ERK1/2 - T202/204) (E10) (Cell Signaling, #9106), ERK1/2/MAP-Kinase (Sigma, #M5670), pY100 (Cell Signaling, #9411), FER (5D2) (Cell Signaling, #4268), pHistone H3(S10) (D2C8)XP (Cell Signaling, #3377), Histone H3 (D1H2)XP (Cell Signaling, #4499), cleaved caspase-3 (Asp175) (Cell Signaling, #9661), PARP1 (Cell Signaling, #9542), Aurora B/AIM-1 (BD Bioscience, #611082), pFAK(T397) (D20B1) (Cell Signaling, #8556), FAK (D2R2E) (Cell Signaling, #130095), MAP4K5 (KHS [E-5]) (Santa Cruz, sc-374070). Secondary antibodies were anti-rabbit and anti-mouse (GE Healthcare).