Whole cell lysates were prepared in RIPA buffer containing protease inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA) as described (36 (link)). Immunoblotting was performed with anti-MUC1-C (#MA5-11202, 1:100; Thermo Fisher Scientific), anti-PBRM1 (A301-591A, 1:10000; Bethyl Laboratories, Montgomery, TX, USA), anti-IRF1 (#8478, 1:1000; Cell Signaling Technology (CST), Danvers, MA, USA), anti-STAT1 (#9172, 1:1000; CST), anti-IDO1 (#86630S, 1:1000 dilution; CST), anti-WARS (GTX110223, 40037, 1:1000; GeneTex, Irvine, CA, USA), anti-RIG-I (#3743, 1:1000; CST), anti-MDA5 (#5321, 1:1000; CST), anti-ISG15 (sc-166755, 1:250; Santa Cruz, Santa Cruz, CA, USA), anti-γH2AX (#9718, 1:1000 dilution; CST), anti-β-actin (A5441; 1:50000; Sigma, St. Louis, MO, USA) and anti-GAPDH (#2118, 1:1000; CST).
Anti wars
Manufactured by GeneTex
Sourced in United States
The Anti-WARS antibody is a laboratory reagent used in research applications. It is designed to specifically detect and bind to the WARS protein, which is involved in the tryptophanyl-tRNA synthetase pathway. The antibody can be utilized in various experimental techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to aid in the study of the WARS protein and its related biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Protease inhibitor cocktail, by Thermo Fisher Scientific (4 mentions)
Anti β actin, by Merck Group (2 mentions)
Anti isg15, by Santa Cruz Biotechnology (2 mentions)
Anti pbrm1, by Fortis Life Sciences (1 mentions)
Anti muc1 c, by Thermo Fisher Scientific (1 mentions)
Ab109617, by Abcam (1 mentions)
Ab180589, by Abcam (1 mentions)
Anti fbxw7, by Abcam (1 mentions)
Anti pbrm1 a301 591a, by Fortis Life Sciences (1 mentions)
Anti muc1 c, by Cell Signaling Technology (1 mentions)
2 protocols using anti wars
1
Immunoblotting Analysis of Cellular Proteins
2
Immunoblotting Analysis of Cell Signaling
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Whole cell lysates were prepared in RIPA buffer containing protease inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA). Immunoblotting was performed with anti-MUC1-C (16564, 1:1000 dilution; Cell Signaling Technology (CST), Danvers, MA, USA), anti-STAT1 (9172S, 1:1000 dilution; CST), anti-IRF1 (#8478S, 1:1000 dilution; CST), anti-IFNGR1 (34808, 1:1000; CST), anti-GAPDH (#5174S, 1:1000 dilution; CST) and anti-β-actin (A5441; 1:100000 dilution; Sigma, St. Louis, MO, USA), anti-FBXW7 (ab109617, 1:1000; abcam, Cambridge, MA, USA), anti-MTA1 (5647, 1:1000; CST), anti-MBD3 (14540, 1:1000; CST), anti-IDO1 (86630, 1:1000; CST), anti-WARS (GTX110223, 40037, 1:1000; Gene Tex), anti-PTGES (ab180589, 1:1000; abcam), anti-PBRM1(A301-591A, 1:10000; Bethyl Laboratories, Montgomery, TX, USA), anti-ARID1A (12354, 1:500; CST), anti-ISG15 (sc166755, 1:2000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-SERPINB9 (PA5-51038, 1:2000; Invitrogen, Waltham, MA, USA).
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