Images were acquired using a Nikon eclipse TS100 brightfield microscope, custom-modified to support fluorescence imaging. A 470 nm LED (cat. no. M470L5, Thorlabs) was mounted at a rear optical port and routed to the objective/camera through a FITC-filter cube, consisting of a 475/35 nm bandpass (excitation) filter (cat. no. MF475-35, Thorlabs), a 530/43 nm bandpass (emission) filter (cat. no. MF540-43, Thorlabs), a 498 nm longpass dichroic mirror (cat. no. MD498, Thorlabs) and assorted optomechanical components for mounting. Images were captured using an Optika CP-6 digital camera (cat. no. 57549) through one of three objective lenses (Nikon Plan 10X 0.25 NA, Nikon Plan Fluor 20X 0.45 NA and Nikon Plan Fluor 40X 0.60 NA).
Eclipse ts100 brightfield microscope
Manufactured by Nikon
Sourced in Japan
The Nikon Eclipse TS100 is a brightfield microscope designed for routine laboratory applications. It features a stable and ergonomic design, providing a reliable platform for various microscopy tasks. The Eclipse TS100 is equipped with high-quality optics, delivering clear and detailed images for a wide range of sample observations.
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Lab products found in correlation
3 protocols using eclipse ts100 brightfield microscope
1
Fluorescence Microscopy Imaging Protocol
2
Live Cell Brightfield and Fluorescence Imaging
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Live cells were imaged using Nikon Eclipse TS100 brightfield microscope on a 40X objective. For fluorescent imaging, cells were fixed using 4% paraformaldehyde/1X PBS for 10 min. The cells were then washed in 1X PBS and subsequently mounted on mounting media containing DAPI (Vectashield H-1200) before being imaged on a Leica SP8 Confocal Microscope.
3
Analyzing Corneal Cell Density in DMEK
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All tissues (n = 12) were analyzed before and after Descemet membrane stripping at the Cornea Department of ETB-BISLIFE to determine the cell density and identify possible denuded areas by using an upright brightfield microscope. After transport, the preloaded DMEK rolls were released into a 35-mm culture dish filled with BSS ophthalmic irrigation solution (Alcon, MO), and prestripped-only DMEK tissues were fully stripped. DMEK tissues for immunofluorescence and histology were stained with 0.4% trypan blue solution for 30 seconds and washed with BSS ophthalmic irrigation solution (Alcon), then unfolded on a glass slide and analyzed with an Eclipse TS100 brightfield microscope (Nikon, Tokyo, Japan) to determine the cell density and denuded areas (n = 6). The tissues for viability assay (n = 6) were immediately incubated with the viability assay.
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