Lentivirus overexpressed and knockdown OLFM4 were designed and purchased from Genechem (Genechem, Shanghai, China). Following 48 h transfection, the expression of OLFM4 was detected by western blotting, and the transfected cells were used for further analysis. Cells transfected with empty lentivirus were used as a negative control. Cells were treated with 15 nM 5'-Aza-deoxycytidine (5-Aza, Sigma‑Aldrich, St. Louis, MO, USA) or 1.5 nM PF-00562271, a FAK inhibitor (Selleck, Houston, TX, USA) for 48 h and then used for further analysis.
Fak inhibitor
Manufactured by Selleck Chemicals
Sourced in United States
FAK inhibitor is a chemical compound that functions as an inhibitor of the Focal Adhesion Kinase (FAK) enzyme. FAK is involved in cellular processes related to cell adhesion, migration, and survival. The FAK inhibitor acts by blocking the enzymatic activity of FAK, thereby modulating these cellular functions.
Automatically generated - may contain errors
Lab products found in correlation
Pf 00562271, by Selleck Chemicals (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
P erk1 2 thr202 tyr204, by Cell Signaling Technology (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Opti mem serum free medium, by Thermo Fisher Scientific (1 mentions)
Vimentin, by Abcam (1 mentions)
Dimethyl sulphoxide dmso, by Merck Group (1 mentions)
E cadherin, by Abcam (1 mentions)
Pd98059, by Selleck Chemicals (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Integrin β1, by Immunoway (1 mentions)
Pf 573228, by Selleck Chemicals (1 mentions)
Gapdh, by Santa Cruz Biotechnology (1 mentions)
2 protocols using fak inhibitor
1
OLFM4 Regulation and Epigenetic Modulation
2
Oridonin Inhibits EMT via FAK/ERK Pathway
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Oridonin was obtained from Aladdin (Shanghai Biochemical Technology Co., Ltd.). PF573228 (FAK inhibitor) and PD98059 (ERK1/2 inhibitor) were purchased from Selleck. Roswell Park Memorial Institute (RPMI) 1640 medium and foetal bovine serum (FBS) was obtained from Grand Island Biological Company. TRIzol reagent was obtained from Invitrogen. 3‐(4,5‐Dimethylthiazol‐2‐yl) ‐2,5‐diphenyltetrazolium bromide (MTT) and dimethyl sulphoxide (DMSO) were purchased from Sigma. Primary antibodies against the following proteins were used: E‐cadherin, vimentin, snail and slug, which were purchased from Abcam Co., Ltd.; FAK, p‐FAK(Tyr397), ERK1/2 and p‐ERK1/2(Thr202/Tyr204), which were obtained from Cell Signaling Technology Co., Ltd.; integrin β1 was purchased from ImmunoWay Biotechnology Company; and β‐actin and GAPDH were purchased from Santa Cruz Biotechnology Co., Ltd. Secondary anti‐mouse and anti‐rabbit antibodies were obtained from Pierce. Opti‐MEM serum‐free medium and lipofectamine RNAiMAX were obtained from Thermo Fisher Scientific.
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