Hamilton syringe series 7000

Mice were anesthetized with an isoflurane mixture (3% for induction, and 1.5% for maintenance), and implanted with bilateral 26-gauge stainless steel cannulae (Plastics One, VA) directed towards the cornus ammonis area-1 (CA1) of dorsal hippocampus (AP: -1.82/DV: -1.7/ML: ±1.3 mm; mm from bregma; 333 nl/5 min) [32 ]. The injector extended 0.2 mm beyond the tip of the cannula. Animals recovered for 10 days. Prior to artificial cerebrospinal fluid (aCSF)/orexin A injections, animals were acclimated to the restraint and injection procedure with 3 days of artificial aCSF infusion. The infusions of either aCSF 0.2 μl per site (Harvard Apparatus, Holliston, MA USA) or orexin A (American Peptide Co., CA) 250 pmol/0.2 μl per site were made using a syringe pump (KD Scientific Inc., MA) coupled to a Hamilton syringe (Series 7000, Sigma-Aldrich, MO). The animals were gently scuffed to place the bilateral injector into the guide cannula and injectate was delivered into both hemispheres simultaneously over 1 min. All injections occurred between 9 and 11 AM.