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Coralite594 conjugated donkey anti rabbit igg h l

Manufactured by Proteintech
Sourced in China, United States

CoraLite594-conjugated donkey anti-rabbit IgG (H + L) is a secondary antibody conjugated with the fluorescent dye CoraLite594. It is designed to detect and visualize rabbit primary antibodies in various immunoassays.

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2 protocols using coralite594 conjugated donkey anti rabbit igg h l

1

Chicken Cell Culture and IBV Detection

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Chicken HD11 and DF-1 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Cellmax, Beijing, China) supplemented with 10% fetal bovine serum (FBS, Cellmax) and 100  IU/mL penicillin–streptomycin solution (Cellmax). All cells were cultured in an incubator at 37 °C in 5% CO2.
The IBV Beaudette strain (GenBank: DQ001339) was kindly gifted by Prof. Ding-Xiang Liu, South China Agricultural University.
Mouse monoclonal antibodies specific for Flag and β-actin, rabbit polyclonal anti-Myc antibodies, CoraLite488-conjugated donkey anti-mouse IgG (H + L), CoraLite594-conjugated donkey anti-rabbit IgG (H + L), HRP-conjugated Affinipure donkey anti-mouse IgG (H + L), and HRP-conjugated Affinipure donkey anti-rabbit IgG (H + L) were purchased from Proteintech (Wuhan, China). Mouse monoclonal anti-IBV N protein antibodies were from Novus Biologicals (Littleton, CO, USA). Rabbit polyclonal anti-chJAK1 and anti-chSTAT1 antibodies were from Abmart (Shanghai, China) and Bioss (Beijing, China), respectively.
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2

Ovarian Dysfunction Mechanism Exploration

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The chemicals and reagents used were the standard substances kaempferol, quercetin, hesperetin, and hesperidin (National Institutes for Food and Drug Control), Cyclophosphamide (Baxter Oncology GmbH, Halle, Germany; No. 8K274A), Dehydroepiandrosterone (General Nutrition Corporation Pittsburgh, PA, USA; No. 61411H18), Rat FSH enzyme-linked immunosorbent assay (ELISA) kit (Cloud-Clone Corp. Wuhan, China; No. CEA830Ra), Rat AMH ELISA kit (Cloud-Clone Corp. Wuhan, China; No. CEA228Ra), Rat GnRH ELISA kit (Cloud-Clone Corp. Wuhan, China; No. CEA843Ra), Rat E2 ELISA kit (Cloud-Clone Corp. Wuhan, China; No. CEA461Ge), TUNEL apoptosis assay kit (KeyGENBio TECH. Jiangsu, China; No. KGA 7072), 4′, 6-diamidino-2-phenylindole (DAPI) staining kit (KeyGENBio TECH. Jiangsu, China; No. KGA 215-10), Normal sheep serum (ZSGB-BIO. Beijing, China; No. ZLI-9022), Triton X-100 (KeyGENBio TECH. Jiangsu, China; No. KGF011), Caspase-1 rabbit polyclonal antibody (Proteintech Group, Inc. Chicago, USA; No. 22915-1-AP), GAPDH mouse monoclonal antibody (Proteintech Group, Inc. Chicago, USA; No. 60004-1-lg), Anti-GSDMD antibody (Abcam Cambridge, MA, USA; No. ab219800), Rat IL-18 ELISA kit (RayBiotech, Inc. Georgia, USA; No. P97636), and CoraLite594-conjugated donkey anti-rabbit IgG (H+L) (Proteintech Group, Inc. Chicago, USA; No. SA00013-8).
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