Mcdb 131 culture medium

HUVECs were purchased from PromoCell. They were grown in MCDB-131 culture medium (Life Technologies, Carlsbad, CA, USA) enriched with L-glutamine 2mM (Gibco^TM, Fisher Scientific, Hampton, NH, USA), foetal bovine serum 7.5% v/v (Gibco^TM, Fisher Scientific, Hampton, NH, USA), Penicillin/Streptomycin 100 U/mL (Gibco^TM, Fisher Scientific, Hampton, NH, USA) and endothelial growth factor 1X (R&D systems^®, Minneapolis, MN, USA). Cells were received at passage 2 and were grown until passage 8. All cells were grown at 37 °C in a humified 5% CO₂ incubator (Fisher Scientific, Hampton, NH, USA).
Generation of immortalized WT VSMCs lines was previously described [57 (link)]. Cell lines were cultured to subconfluence (70–80%) with 10% foetal bovine serum (FBS)-DMEM for in vitro experiments.

HUVECs were purchased from PromoCell. They were grown in MCDB‐131 culture medium (Life Technologies, Carlsbad, CA, USA) enriched with l‐glutamine 2 mM (GibcoTM, Fisher Scientific, Hampton, NH, USA), foetal bovine serum (FBS) 7.5% v/v (GibcoTM, Fisher Scientific, Hampton, NH, USA), Penicillin/Streptomycin 100 U/mL (GibcoTM, Fisher Scientific, Hampton, NH, USA) and endothelial growth factor 1X (R&D systems^®, Minneapolis, MN, USA). Cells were received at passage 2 and were grown until passage 5. All cells were grown at 37°C in a humidified 5% CO₂ incubator (Fisher Scientific, Hampton, NH, USA).
Generation of immortalized WT VSMCs lines was previously described.¹⁷ Cell lines were cultured to subconfluence (70%−80%) with 10% FBS‐DMEM for in vitro experiments.