DOC, 8-anilinonapthalene-1-sulfonic acid (ANS), naringenin, LTA from Staphylococcus aureus, and LPS from Escherichia coli O111:B4 were purchased from Sigma-Aldrich, Inc. (St. Louis, MO, USA); Kdo2-Lipid A (Kdo2) from Adipogen, Inc. (Songdo-dong, Yeonsu-gu, Incheon, South Korea) or Avanti Polar Lipids, Inc. (Alabaster, AL, USA); and quercetin 3-O-sophoroside (Q3OS) from Haihang Industry Co., Ltd. (Jinan City, China). PPE1, B1-phytoprostanes (PPB1), F1-phytoprostanes (PPF1), and an isomeric mixture consisting of B1-, E1-, and F1-phytoprostanes (PPmix) were produced by autoxidation of α-linolenic acid, as described elsewhere 23 (link). Type I or/and type II phytoprostanes were used, as indicated in Figure 4C . Unless otherwise stated, Bet v 1 was mixed with each of the six ligands in a 1:10 molar ratio and incubated either overnight at 4°C or for 2 h at room temperature. A1-phytoprostanes (PPA1) were purchased from Cayman Chemicals (Ann Arbor, MI, USA) and dried and dissolved in DMSO.
A1 phytoprostanes ppa1
Manufactured by Cayman Chemical
Sourced in United States
A1-phytoprostanes (PPA1) is a chemical compound used in laboratory research. It serves as a reference standard for the identification and quantification of phytoprostanes, which are plant-derived metabolites. The core function of A1-phytoprostanes (PPA1) is to provide a reliable and consistent reference point for analytical methods used to study phytoprostanes.
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2 protocols using a1 phytoprostanes ppa1
1
Ligand-Induced Conformational Changes in Bet v 1
2
Binding Assays for Bet_v_1 Interactions
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DOC, 8‐anilinonaphthalene‐1‐sulfonic acid (ANS), naringenin, LTA from Staphylococcus aureus, and LPS from Escherichia coli O111:B4 were purchased from Sigma‐Aldrich, Inc; Kdo2‐Lipid A (Kdo2) from Adipogen, Inc or Avanti Polar Lipids, Inc; and quercetin 3‐O‐sophoroside (Q3OS) from Haihang Industry Co., Ltd. PPE1, B1‐phytoprostanes (PPB1), F1‐phytoprostanes (PPF1), and an isomeric mixture consisting of B1‐, E1‐, and F1‐phytoprostanes (PPmix) were produced by autoxidation of α‐linolenic acid, as described elsewhere.23 Type I or/and type II phytoprostanes were used, as indicated in Figure 4 C. Unless otherwise stated, Bet_v_1 was mixed with each of the six ligands in a 1:10 molar ratio and incubated either overnight at 4°C or for 2 hours at room temperature. A1‐phytoprostanes (PPA1) were purchased from Cayman Chemicals and dried and dissolved in DMSO.
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