Total RNA from serum of AKI patients were extracted with TRIzol reagent (Invitrogen, Carlsbad, CA, USA). Then 3 µg total RNA were used remove ribosomal RNA, and retain RNA classes including noncoding RNAs with VAHTS Total RNA-seq (H/M/R) Library Prep kits from Illumina (Vazyme Biotech Co., Ltd, Nanjing, China). We treated RNA through 40 U RNase R (Epicenter) at 37 °C for three hours, followed by TRIzol purification. We prepared RNA-seq libraries via KAPA Stranded RNA-Seq Library Prep kits (Roche, Basel, Switzerland) and used them for deep sequencing (Illumina HiSeq 4000 at Aksomics, Inc., Shanghai, China).
Total rna seq h m r library prep kit
Manufactured by Illumina
Sourced in China
The Total RNA-seq (H/M/R) Library Prep Kit is a laboratory equipment product designed for the preparation of sequencing libraries from total RNA samples. The kit is compatible with human, mouse, and rat species. The core function of the product is to enable the generation of sequencing-ready libraries from total RNA for subsequent analysis.
Automatically generated - may contain errors
Lab products found in correlation
Hiseq 4000, by Illumina (4 mentions)
Trizol reagent, by Thermo Fisher Scientific (4 mentions)
Kapa stranded rna seq library prep kit, by Roche (4 mentions)
Rnase r, by Illumina (3 mentions)
Qiaamp rna blood mini kit, by Qiagen (2 mentions)
Novaseq 6000, by Illumina (2 mentions)
Hipure total rna mini kit, by Magen Biotechnology Co (1 mentions)
2100 bioanalyzer, by Agilent Technologies (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Rnase r, by Thermo Fisher Scientific (1 mentions)
7 protocols using total rna seq h m r library prep kit
1
RNA-seq pipeline for serum ncRNAs
2
Exosomal RNA Sequencing of Hypoxic ADSCs
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Total RNA was obtained from hypoxia pretreated ADSC exosomes (HExo) and Exo by using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). Approximately ~3 μg total RNA from every sample was processed with VAHTS Total RNA-seq (H/M/R) Library Prep kits from Illumina (Vazyme Biotech Co., Ltd, Nanjing, China) to remove ribosomal RNA and retain RNAs including mRNAs and noncoding RNAs. The RNA was treated with 40 U RNase R (Epicenter) at 37°C for 3 h, and TRIzol purification was performed. An RNA-seq library was constructed with KAPA Stranded RNA-Seq Library Prep kits (Roche, Basel, Switzerland) and used for NGS (Illumina HiSeq 4000, Aksomics, Inc., Shanghai, China).
3
Orbital Connective Tissue RNA-Seq
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Total RNA was extracted from orbital connective tissue with a HiPure Total RNA Mini Kit (Magen, Guangzhou, China) according to the manufacturer’s protocol. Libraries were constructed using the Total RNA-seq (H/M/R) Library Prep Kit for Illumina®. Genes showing altered expression with p < 0.05 and more than 1.5-fold changes were considered differentially expressed.
4
Total RNA Sequencing from PBMCs
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Total RNA was isolated from the PBMC samples using the QIAamp RNA Blood Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions. Total RNA (1 µg) was used to prepare a sequencing library. PolyA‐tailed RNAs were selected using mRNA capture beads (VAHTS) and the Illumina Total RNA‐seq (H/M/R) Library Prep Kit was used according to the manufacturer's instructions. Library quality was examined using an Agilent Bioanalyzer 2100. The libraries were pooled in equimolar amounts to a final concentration of 2 nm . The normalized libraries were denatured with 0.1 m NaOH (Sigma) and the pooled denatured libraries were pair‐end sequenced with a 150‐bp read length in the Illumina NovaSeq 6000 platform.
5
Comprehensive RNA-seq Analysis of Breast Cancer
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We extracted total RNA from paired BC and adjacent noncancerous tissues with TRIzol reagent (Invitrogen, Carlsbad, CA, USA). We subjected nearly 3 μg of total RNA from each sample to the VAHTS Total RNA-seq (H/M/R) Library Prep Kit from Illumina (Vazyme Biotech Co., Ltd., Nanjing, China) to erase ribosomal RNA, but retain other RNA classes such as non-coding RNAs and mRNAs. We treated the RNAs with 40 U of RNase R (Epicenter) at 37 °C for 3 h, followed by TRIzol purification. We prepared RNA-seq libraries by the KAPA Stranded RNA-Seq Library Prep Kit (Roche, Basel, Switzerland) and subjected them to deep sequencing through Illumina HiSeq 4000 at Aksomics, Inc. (Shanghai, China). For miRNA and mRNA analyses, T24 cells transfected with siRNA against hsa_circ_0001944 or negative control (NC) vector were used for high-throughput RNA-Seq of miRNAs as previously mentioned.
6
Total RNA Extraction and Illumina Sequencing
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Total RNAs were extracted from PBMC samples using a QIAamp RNA Blood Mini kit (Qiagen, Valencia, CA) according to the manufacturer’s instructions. Total RNA (1 μg) was used for sequencing library preparation, and the Illumina Total RNA-seq (H/M/R) Library Prep Kit was then used according to manufacturer’s instruction. We used an Agilent Bioanalyzer 2100 to examine library quality. The libraries were pooled together in equimolar amounts to a final concentration of 2 nM. The normalized libraries were denatured with 0.1 M NaOH (Sigma), and pooled denatured libraries were pair-end sequenced with a 150 bps read length on the Illumina NovaSeq 6000 platform.
7
RNA-seq Analysis of Myasthenia Gravis
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Total RNA was extracted from sera of patients with and without MG using TRIzol reagent (Invitrogen). Approximately 3 μg of total RNA was restricted from each sample using the VAHTS Total RNA‐seq (H/M/R) Library Prep Kit from Illumina (Vazyme Biotech Co., Ltd.) to remove ribosomal RNA, while preserving other RNA classes, including noncoding RNAs and mRNAs. RNAs were treated with 40 U of RNase R (Invitrogen) at 37°C for 3 h, and purified with TRIzol. RNA‐seq libraries were generated using a KAPA Stranded RNA‐Seq Library Prep Kit (Roche), and subjected to deep sequencing using an Illumina HiSeq. 4000 (Aksomics, Inc.).
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