Rabbit anti syntenin 1

Manufactured by Abcam

Sourced in United Kingdom, United States

Rabbit anti-syntenin 1 is a primary antibody that specifically recognizes syntenin 1, a scaffolding protein involved in various cellular processes. This antibody can be used for the detection and analysis of syntenin 1 expression in biological samples.

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Lab products found in correlation

Rabbit anti tsg101, by Abcam (1 mentions) Ecl detection reagent, by GE Healthcare (1 mentions) Lms710, by Zeiss (1 mentions) Alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Mouse anti β 3 tubulin, by BioLegend (1 mentions) Rabbit anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Dm2500, by Leica (1 mentions) Hrp conjugated donkey anti mouse igg, by Jackson ImmunoResearch (1 mentions) Mouse anti rpe65, by Novus Biologicals (1 mentions) Anti sod1, by Proteintech (1 mentions) Hrp conjugated donkey anti rat igg, by Jackson ImmunoResearch (1 mentions) Hrp conjugated donkey anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Rabbit anti calreticulin, by Cell Signaling Technology (1 mentions) Anti catalase, by Proteintech (1 mentions) Hoechst 33258, by Thermo Fisher Scientific (1 mentions) Triton x 100, by Merck Group (1 mentions)

Close spelling variants of this product

Rabbit anti‐syntenin Anti-syntenin-1 Syntenin-1 Anti-syntenin Syntenin Rabbit anti-

3 protocols using rabbit anti syntenin 1

Western Blot Analysis of EV Markers

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Samples were run on NuPAGE 10–12% Bis-Tris gels (Life Technologies, Paisley, UK). The following primary antibodies were used: rabbit anti-flotillin (1:1000 Abcam, Cambridge, UK), rabbit anti-Tsg101 (Abcam 1:250), rabbit anti-syntenin 1 (Abcam, 1:1000). Blots were visualized using HRP-conjugated secondary antibodies and the ECL Detection Reagent (GE Healthcare, Little Chalfont, UK).

Tomlinson P.R., Zheng Y., Fischer R., Heidasch R., Gardiner C., Evetts S., Hu M., Wade-Martins R., Turner M.R., Morris J., Talbot K., Kessler B.M, & Tofaris G.K. (2015). Identification of distinct circulating exosomes in Parkinson's disease. Annals of Clinical and Translational Neurology, 2(4), 353-361.

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Quantification of Apoptosis in Cortical Neurons

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Cortical neurons were plated, fixed, and stained with mouse anti-β-III tubulin (1:1000, BioLegend, Dedham, MA, USA), rabbit anti-cleaved caspase 3 (1:400, CellSignaling Technology, Danvers, MA, USA), and rabbit anti-syntenin 1 (Abcam, 1:2000). Goat anti-mouse AlexaFluor488 and goat anti-rabbit AlexaFluor568 (both Invitrogen, 1:500) were used as secondary antibodies and cells were imaged with a fluorescent (DM2500, Leica, Solms, Germany) or confocal (LMS710, Zeiss, Oberkochen, Germany) microscope. For quantification of caspase three positive neurons, digital photographs of five random fields were taken (Rotera XR Fast 1394; QImaging, Surrey, UK) and the percentage of caspase three positive, β-III tubulin-positive neurons were counted in an unbiased manner. All experiments were repeated three times and presented data were based on a total count of at least 2500 neurons per condition per experiment.

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Antibody Characterization in Cell Research

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Calcium and magnesium free PBS (PBS; #10010‐023), and Hoechst 33258 (#H3569) were from Invitrogen (Waltham, MA). Triton X‐100 (#T8787) was obtained from Sigma–Aldrich (St. Louis, MO). Antibodies used in the current study were as follows: Mouse anti‐RPE65 (#NB100‐355) [clone 401.8B11.3D9]; Novus Biologicals, Centennial, CO), mouse anti‐DSG1 (#610273, BD Transduction, Franklin Lakes, NJ), mouse anti‐Cytokeratin 10 (#MA5‐13705 [clone DE‐K10]; ThermoFisher, Waltham, MA), rabbit anti‐Syntenin‐1 (#ab19903]; Abcam, Cambridge, MA), mouse anti‐Annexin II (#610068, BD Transduction Laboratories, Franklin Lakes, NJ), rat anti‐Integrin Beta 1 (AIIB2 clone, Developmental Studies Hybridoma Bank, Iowa City, IA), mouse anti‐Occludin (#66378‐1‐Ig, Proteintech, Rosemont, IL), rabbit anti‐Calreticulin (#12238 [clone D3E6]; Cell Signaling Technologies, Danvers, MA), anti‐SOD1 (#10269‐1‐AP, Proteintech), anti‐Catalase (#66765‐1‐Ig, Proteintech), anti‐MDA (#NBP2‐59367, Novus Biologicals), anti‐HSPA5 (#PA5‐19503, ThermoFisher), anti‐CLDN19 (#SC‐36597, Santa Cruz Technology, Dallas, TX), HRP‐conjugated donkey‐anti‐rat IgG (#712‐035‐153, Jackson ImmunoResearch Laboratories, West Grove, PA), HRP‐conjugated donkey‐anti‐mouse IgG (#715‐035‐150, Jackson ImmunoResearch) and HRP‐conjugated donkey‐anti‐rabbit IgG (#711‐035‐152, Jackson ImmunoResearch).

Hernandez B.J., Skiba N.P., Plössl K., Strain M., Liu Y., Grigsby D., Kelly U., Cady M.A., Manocha V., Maminishkis A., Watkins T., Miller S.S., Ashley‐Koch A., Stamer W.D., Weber B.H., Bowes Rickman C, & Klingeborn M. (2023). Polarized desmosome and hemidesmosome shedding via small extracellular vesicles is an early indicator of outer blood‐retina barrier dysfunction. Journal of Extracellular Biology, 2(10), e116.

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