For analysis of isolated compounds, an HPLC ekspert ultraLC 100 coupled with a TripleTOF 5600 mass spectrometer (AB SCIEX, Framingham, MA, USA) was used. For chromatographic separation, a Shim-pack XR-ODS C18 column (5 cm × 2.0 mm, 2.2 µm particle size) (Shimadzu, Kyoto, Japan) was used, coupled with a pre-column with the same material. The mobile phases were water and methanol (both with 0.1% of formic acid). The elution method initiated with 5% of methanol was kept isocratic for 1.0 min, which was then increased to 95% over 10 min. Four minutes were added to the method to wash and stabilize the column. The injection volume was 2 µL, and the column temperature was 40 °C. The positive ionization mode was employed and the ionization source parameters were CUR 22 (Curtain Gas), source temperature 450 °C, ionization voltage (IS) 5500 V, Gas1 (nebulization gas) 45, Gas2 (turbo heaters gas) 45 and DP 100 (declustering potential).
Ekspert ultralc 100
Manufactured by AB Sciex
Sourced in United States, Japan
The Ekspert ultraLC 100 is a high-performance liquid chromatography (HPLC) system designed for analytical and preparative applications. It features a modular design, allowing customization to meet specific laboratory requirements. The system is capable of delivering precise and reliable solvent delivery, sample handling, and data acquisition, enabling accurate and reproducible chromatographic separations.
Automatically generated - may contain errors
3 protocols using ekspert ultralc 100
1
HPLC-MS/MS Analysis of Isolated Compounds
2
Quantitative LC-MS/MS for Bioanalysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liquid‐chromatography tandem mass spectrometry (LC‐MS/MS) analyses were performed using an API 3200 LC‐MS/MS system coupled to ekspert ultra LC 100 (AB Sciex, Tokyo, Japan). An octadecylsilyl silica (ODS) column (TSKgel ODS‐100Z; 2.0 mm I.D. ×50 mm; TOSOH, Tokyo, Japan) was used and maintained at ambient temperature. The gradient elution method used for chromatographic separation is shown in Table S2 .
The mass spectrometer was operated in the electrospray positive ionization mode, and system control and data acquisition were performed using Analyst software version 1.6.1 (AB Sciex). The settings were set as follows: ion spray source temperature at 500°C, ion spray voltage at 5500 V, curtain gas at 10, ion source gas 1 at 80, ion source gas 2 at 60, and collision gas at 4. Sample analysis was performed in the multiple reaction monitoring mode; the optimized conditions for monitoring the m/z of parent and product ions are shown in TableS3 .
The mass spectrometer was operated in the electrospray positive ionization mode, and system control and data acquisition were performed using Analyst software version 1.6.1 (AB Sciex). The settings were set as follows: ion spray source temperature at 500°C, ion spray voltage at 5500 V, curtain gas at 10, ion source gas 1 at 80, ion source gas 2 at 60, and collision gas at 4. Sample analysis was performed in the multiple reaction monitoring mode; the optimized conditions for monitoring the m/z of parent and product ions are shown in Table
3
UHPLC-based Chromatographic Separation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chromatographic separation was performed by UHPLC system (Eksigent Ekspert ultra LC 100, AB SCIEX, CA) consisting of a binary solvent manger, a sample manager and a column compartment. Chromatographic separation was undertaken on an HSS C18 column (100 mm × 2.1 mm i.d., 1.8 μm, Waters Corporation, Milford, USA). The column temperature was maintained at 40 °C. The flow rate was 0.4 ml/min. The injected sample volume was 3 μl for each run. All the samples were kept at 4 °C during the analysis. The optimal mobile phase containing a linear gradient elution program of 0.1% formic acid in water (solvent A) and 0.1% formic acid in acetonitrile (solvent B) was performed as follows: 0 min at 99% A; 0−5.5 min at 99–62% A; 5.5–7.5 min at 62–50% A; 7.5–9.0 min at 50–34% A; 9.0–13.0 min at 34–100% A; 13.0–15.0 min at 0–0% A.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!