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3 protocols using sodium oleate

1

Synthesis and Characterization of Gold Nanoparticles

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General reagents were purchased from Nacalai Tesque (Kyoto, Japan). Gold(III) chloride, sodium borohydride, PSS, PDDAC, PEI, and PLL were purchased from Sigma-Aldrich (Saint Louis, MO, USA). Silver nitride, L(+)-ascorbic acid, sodium oleate, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), sodium cholate, Gold ICP-MS Standard, and the lactate dehydrogenase (LDH) assay kit were obtained from Wako (Osaka, Japan). Rho-PE and DOTAP were purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). Opti-MEM, LF2000, LysoTracker Green DND-26 (LysoTracker), and fetal bovine serum (FBS) were obtained from Life Technologies (Carlsbad, CA, USA). Escherichia coli strain BL21 was purchased from Novagen (Madison, WI, USA). Spectra/Por Dialysis membranes (MWCO 50 kDa) were purchased from Spectrum Laboratories (Rancho Dominguez, CA, USA). NAP-5 columns were purchased from GE Healthcare UK Ltd. (Buckinghamshire, UK). Cell culture dishes and trypsin/EDTA were obtained from BD Biosciences (San Jose, CA, USA). Glass-based dishes with or without grid lines were purchased from Matsunami Glass Co. Ltd. (Osaka, Japan). Annexin V-FITC and propidium iodide were obtained from Funakoshi (Tokyo, Japan). Cell Counting Kit-8 was obtained from Dojindo (Kumamoto, Japan).
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2

Adsorption and Adhesion Behavior of CNF

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To evaluate the adsorption behavior, the CNF suspension (10 µl) was dropped onto a HAp plate (10 × 10 × 1 mm3; HOYA Corp., Tokyo, Japan) and after washing with distilled water and NaCl solutions with higher ionic strengths (1%), the remained molecules on the HAp plate were determined by using an attenuated total reflection Fourier transform infrared spectrophotometer (IRAffinity-1S, Shimadzu Corp., Kyoto, Japan). To evaluate the adhesion strength, the CNF suspensions (0–1000 mg/ml) were placed between two HAp plates and dried at 37°C for 6 h. The adhesion strength between two HAp plates was measured using a universal mechanical tester (EZ test, Shimadzu Corp.). Since the fatty acid is one of the major components of cellular membrane, sodium oleate (FUJIFILM Wako Pure Chemical Corp.) was also used for the adsorption (10 mg/ml) and adhesion (0–200 mg/ml) experiments as described above.
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3

Biomass Characterization and Crude Glycerol Modeling

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Samples of Japanese cedar (Cryptomeria japonica), spruce (Picea sitchensis), oak (Quercus crispula), beech (Fagus crenata), bamboo, and rice straw were selected for different types of lignocellulosic biomass. Planer dust of Japanese cedar was obtained from the Miyazaki Prefectural Forestry Technology Center (Misato town, Japan). Bamboo powder was obtained from the Fukuoka Industrial Technology Center in Okawa, Fukuoka Prefecture. Oak was purchased from Kose (Fukui City, Japan). Rice straw was obtained from a rice field in Kushima City, Miyazaki Prefecture. Spruce and beech were purchased from a sawmill in Fukuoka Prefecture. The samples were sieved to give a size distribution of 177-350 μm (42-80 mesh).
A model of crude glycerol was made by mixing glycerol and a fatty acid sodium salt such as sodium laurate, sodium stearate, sodium oleate (all from Fujifilm Wako Pure Chemical, Osaka, Japan), or sodium linoleate (Tokyo Chemical Industry, Tokyo, Japan). Sodium acetate and oleic acid (both from Fujifilm Wako Pure Chemical) were also used to investigate the impact of carbon chain length and alkalinity. Abbreviations of the sodium salts and fatty acids used are shown in Table 1.
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