The porous structure of the membranes was investigated by scanning electron microscopy (SEM). SEM was performed using the SU8020 microscope with a cold cathode (Hitachi, Japan). In order to improve the resolution and contrast of images, a thin layer of the gold–palladium alloy was deposited on the samples. For the determination of the surface pore diameter, all SEM images were recorded under identical conditions. The transverse size of pore openings was measured using the GATAN DIGITAL MICROGRAPH software. For each sample, 50–80 pores were analyzed. Cross-sections of the membranes were prepared after embrittlement with UV for about 100 h in air [47 (link)].
Micrograph software
Manufactured by Ametek
Micrograph software is a digital imaging tool that enables the analysis and processing of microscopic images. It provides core functionalities for image capture, enhancement, and quantitative measurements.
Automatically generated - may contain errors
Lab products found in correlation
H 7000 electron microscope, by Hitachi (2 mentions)
High resolution 4 k 4 k digital camera, by Ametek (2 mentions)
Ultracut e, by Leica (2 mentions)
Su8020 microscope, by Hitachi (1 mentions)
Talos 200x system, by Thermo Fisher Scientific (1 mentions)
Sigma 500, by Zeiss (1 mentions)
Jem 3200fsc field emission microscope, by JEOL (1 mentions)
5 protocols using micrograph software
1
Membrane Pore Structure Analysis
2
Ultrastructural Analysis of Cardiac Tissue
3
Ultrastructural Analysis of Cardiac Tissue
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Small cubic pieces ≤ 1 mm3 were dissected from left ventricles and fixed with 2.5% glutaraldehyde in 0.1 mol/ L sodium phosphate (pH 7.4) overnight at 4 °C. After post-fixation in 1% OsO4, samples were dehydrated through graded alcohols and embedded in Epon Araldite. Ultrathin sections (50 nm) were cut using an ultramicrotome (Ultracut E, Leica), and stained with uranyl acetate and lead citrate. The specimens were viewed on a Hitachi H-7000 Electron Microscope (Pleasanton, CA, USA). Images were captured using a Gatan high resolution 4 k × 4 k digital camera and Gatan Ditital Micrograph software (22 (link)).
4
Nanomaterial Characterization by SEM and TEM
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SEM images are acquired with a Zeiss Sigma 500 scanning electron microscope operating at 3 keV EHT voltage. Samples are loaded into a sample holder, heat sealed in an aluminum-lined blue bag, and brought to the SEM for rapid transfer with minimal air exposure. Images are analyzed using ImageJ software to determine NW length.
TEM images are obtained at a 200 keV acceleration voltage on a Thermo Fisher Talos 200X system. Small amounts of sample powder are dropped on a copper-coated TEM grid which is loaded into the sample holder in an argon glovebox, heat sealed in an aluminum-lined blue bag, brought to the TEM and inserted under argon gas flow. Images, lattice parameters and electron diffraction patterns are analyzed using the Gatan Micrograph software and fast Fourier transform (FFT) tools.
TEM images are obtained at a 200 keV acceleration voltage on a Thermo Fisher Talos 200X system. Small amounts of sample powder are dropped on a copper-coated TEM grid which is loaded into the sample holder in an argon glovebox, heat sealed in an aluminum-lined blue bag, brought to the TEM and inserted under argon gas flow. Images, lattice parameters and electron diffraction patterns are analyzed using the Gatan Micrograph software and fast Fourier transform (FFT) tools.
5
TEM Imaging of Gel Samples
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The transmission electron microscopy (TEM) images were collected using FEI Tecnai G2 operated at 120 kV and JEM 3200FSC field emission microscope (JEOL) operated at 300 kV in bright field mode with Omega-type Zero-loss energy filter. The images were acquired with GATAN DIGITAL MICROGRAPH software. The TEM samples were prepared by placing 3.0 µL of the gel on to a 300 mesh copper grid with holey carbon support film. The TEM grids were plasma cleaned prior to use. The samples were dried under ambient condition prior to imaging.
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