Liver tissues were fixed before embedding in paraffin and for cryosections, tissues were cryoprotected in 30 % sucrose for 24 h before embedding in OCT compound and freezing in liquid nitrogen. For haematoxylin and eosin staining (Sigma), 5 μm paraffin sections of liver tissues were used. Slides were placed in an oven (55 °C) for 10 min, deparaffinized in xylene twice for 15 min, rehydrated by placing in dilutions of ethanol ranging from 100 % to 70 %. Slides were then immersed in distilled water. Nuclei were stained by dipping slides in haematoxylin for 1 min. Slides were rinsed in water and excess haematoxylin was removed with 1 % hydrochloric acid in 70 % ethanol then transferred to water. Slides were then dipped several times in eosin, rinsed and dehydrated with ethanol (from 95 % to 100 %) and finally immersed in xylene. Stock solutions of Oil Red O (Fluka Chemica) were prepared using 0.5 g of Oil Red O and 100 ml of propan-2-ol. The working solutions contained 30 ml of stock solution diluted in 20 ml of distilled water. Slides were incubated in Coplin jars with Oil Red O working solution for 15 min then rinsed with propan-2-ol followed by haematoxylin staining five times and washing with distilled water.
Haematoxylin and eosin staining
Manufactured by Merck Group
Sourced in China
Haematoxylin and eosin (H&E) staining is a widely used laboratory technique used to stain and differentiate cellular and tissue structures. Haematoxylin stains cell nuclei blue, while eosin stains the cytoplasm and other cellular components pink or red. This staining method provides contrast to enable the visualization and identification of various cell types and tissue structures under a microscope.
Automatically generated - may contain errors
Lab products found in correlation
Lsm800 inverted confocal microscope, by Zeiss (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Roche (1 mentions)
F4 80, by Thermo Fisher Scientific (1 mentions)
Gold antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
3 protocols using haematoxylin and eosin staining
1
Histological Examination of Liver Tissue
2
Multifunctional Wound Dressing Synthesis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hyaluronic acid (HA, molecular weight <10 kDa) was purchased from Freda Biochem Co., Ltd. (Shandong, China). Silk peptide (Protein hydrolyzates, molecular weight is between 500 and 1,000 Da) was purchased from Shanghai McLean Biochemical Technology Co., Ltd. (Shanghai, China). Octadecylamine was purchased from Aladdin reagent Co., Ltd. (Shanghai, China). (3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCL) and N-hydroxysuccinimide (NHS ) were purchased from Nanjing dulai Biotechnology Co., Ltd. (Nanjing, China). Curcumin was purchased from Ivy Biotechnology Co. Ltd. (Xian, China). Haematoxylin and Eosin staining were purchased from Sigma-Aldrich (Shanghai, China). All other reagents were analytical grade preparation.
3
Histological Analysis of Adipose Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Adipose tissues were fixed in 4% formalin PBS solution for 24 hrs, dehydrated, embedded in paraffin and sectioned at 5 μm. The sections were deparaffinized, re-hydrated and then subjected to haematoxylin and eosin staining (Sigma) or immunofluorescence staining. For immune-staining, the sections were blocked in 3% BSA (Roche), 10% FBS (Gibco), PBS, pH = 7.4 for 1 hr, followed by incubation with primary antibodies in PBST (PBS, 0.1% Tween 20) containing 3% BSA overnight at 4 °C. The next day, the slides were washed three times in PBST followed by incubation with a fluorophore-conjugated secondary antibody for 1 hr at dark. Slides were counterstained with Gold Antifade Mountant with DAPI (Thermo Fisher Scientific) and visualized with an Olympus biological microscope BX41, and images were captured with an Olympus DP72 color digital camera (for HE staining) and Carl Zeiss LSM800 inverted confocal microscope (immunofluorescence staining). The antibodies used include F4/80 (1:100, Thermo Fisher Scientific, #14-4801-85, BM8), iNOS (1:250, Thermo Fisher Scientific, #PA3-030A), HIF-1α (1:400, Proteintech, 20960-1-AP), CD14 (1:100, Biolegend, #325604, HCD14), Chicken anti-Rat IgG (1:200, Thermo Fisher Scientific, #A21470) and Goat anti-Rabbit IgG (1:200, Thermo Fisher Scientific, #A11011).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!