Pc9 lung

HCT116 colon, MCF7 breast, MDA-MB-231 breast, A375 melanoma, and PC9 lung were purchased from ATCC, were they were validated. HCT116 AKT1/2^−/− was purchased from Horizon Discovery (Cambridge, UK), where it was validated. AG11726 skin fibroblasts were purchased from Coriell Repositories, where they were validated. MCF7, MDA-MB-231 and AG11726 were maintained in DMEM, 10% FCS, 40mM glutamine, 100 U/mL penicillin, and 100μg/mL streptomycin; HCT116 and HCT116 AKT1/2^−/− in McCoy’s 5α medium supplemented with 10% FCS, 100U/mL penicillin, and 100μg/mL streptomycin; PC9 in RPMI, 25% glucose, 1% sodium pyruvate, 100U/mL penicillin, and 100μg/mL streptomycin; A375 in DMEM supplemented with high glucose HEPES buffer, 10% FCS, 100U/mL penicillin, and 100μg/mL streptomycin. All the cells were grown at 37°C and 5% CO₂.

A375 melanoma, PC9 lung, MDA-MB-231 breast, HCT116 colon, and MCF7 breast human cancer cell lines were purchased from ATCC, where they were validated. HCT116 AKT1/2^−/− was purchased from Horizon Discovery, where it was validated. A375, MDA-MB-231, and MCF7 were maintained in DMEM, 10% FCS, 4 mM glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin; HCT116 and HCT116 AKT1/2^−/− in McCoy’s 5α medium supplemented with 10% FCS, 100 U/mL penicillin, and 100 μg/mL streptomycin; PC9 in RPMI, 10% FCS, 25% glucose, 1% sodium pyruvate, 100 U/mL penicillin, and 100 μg/mL streptomycin. All the cells were grown at 37°C and 5% CO₂.