After 24 h transfection, complete growth medium was replaced by serum-free medium. The cell migration assay was performed in 24-well transwell plates with 8.0-µm pore size membrane inserts (Corning, New York, NY, USA) whereas the in vitro invasion assay was performed using the BD BioCoat Matrigel Invasion Chamber kit (Becton-Dickinson, Franklin Lakes, NJ, USA) as previously described.9 (link)
8.0 m pore size membrane inserts
Manufactured by Corning
Sourced in United States
The 8.0-µm pore size membrane inserts are a laboratory equipment designed for cell culture applications. The inserts feature a porous membrane with a uniform pore size of 8.0 micrometers, allowing for the selective passage of cells, media, and other small molecules while retaining larger components. This product is intended for use in various cell-based assays and experiments that require separation or co-culture of different cell types.
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Lab products found in correlation
2 protocols using 8.0 m pore size membrane inserts
1
Cell migration and invasion assays
2
Evaluating Cell Migration in Pancreatic Cancer
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Briefly, FAM172AsiRNA and siRNAcontrol transfected AsPC-1 and BxPC-3 cells (pretreated with 20 µM of PD98059 for 2 h only once) was seeded onto 8.0-µM pore size membrane inserts (Corning Inc., NY, USA) coated with matrigel (BD Biosciences, Sparks, MD, USA) in 24-well plates with FBS-free growth media. Growth media plus 10% FBS was added to the bottom wells as a chemoattractant. 24 h later, cells that moved to the underside of the inserts were stained with Crystal Violet Hydrate (Sigma-Aldrich, St Louis, MO, USA). The migratory cells were counted in five random fields per well. Results were expressed as cells migrated per field and repeated three times. The transwell assay was performed in a similar way without matrigel.
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