SEM morphological characterization was performed on unprocessed pollen grains and processed SECs by employing an FESEM 7600F instrument (JEOL, Japan). Processed and unprocessed samples were coated with platinum at a thickness of 10 nm with JFC-1600 (JEOL, Japan) (20 mA, 60 sec). Samples were observed using an acceleration potential of 5.00 kV at different magnifications. In order to capture cross-sectional images of the samples, pollen grains were subjected to cryogenic freezing in liquid nitrogen. The fixated samples were then diced with a surgical blade and coated with platinum for observation.
Fesem 7600f
Manufactured by JEOL
Sourced in Japan
The FESEM 7600F is a field emission scanning electron microscope (FESEM) manufactured by JEOL. It is designed to provide high-resolution imaging of samples at the nanoscale level. The FESEM 7600F utilizes a field emission gun to generate a focused electron beam, which is then scanned across the surface of the sample to create an image.
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Lab products found in correlation
Jfc 1600, by JEOL (3 mentions)
Lsm 710 confocal microscope, by Zeiss (3 mentions)
Axis supra xps, by Shimadzu (1 mentions)
D8 advance, by Bruker (1 mentions)
Paraformaldehyde (pfa), by Thermo Fisher Scientific (1 mentions)
Jfc 1600 sputter coater, by JEOL (1 mentions)
Axio observer z1 inverted microscope, by Zeiss (1 mentions)
Zen software, by Zeiss (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Rm 1000 invia, by Renishaw (1 mentions)
Jem 3010, by JEOL (1 mentions)
Zetasizer zs, by Malvern Panalytical (1 mentions)
9 protocols using fesem 7600f
1
Pollen Grain Morphology via SEM
2
Multimodal Analysis of Solid and Waxy Material Properties
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Thermogravimetric analysis (TGA) was applied to determine the water content in the solid and waxy states. Fourier‐transform infrared spectroscopy measurement was conducted by Frontier MIR spectrometer (Shimadzu Trace‐100) with the attenuated total reflectance (ATR) compartment. Small‐wide angle X‐ray scattering was performed by SWAXS Nano‐inXider (Xenocs), where the liquid and gel samples were sealed in borosilicate glass, and the wax and solid samples were by customized Kapton‐sealed holder to prevent water loss under vacuum. X‐ray diffraction (XRD) patterns were obtained by using a Bruker D8 Advance powder diffractometer with Cu‐Kα radiation (λ = 1.5406 Å). SEM study was performed by FESEM 7600F (JEOL) equipped with an Electron back Scattering detector and EDS detector (Oxford). XPS spectra were collected by Kratos AXIS Supra XPS with dual anode (Al/Ag Kα) X‐ray monochromatic source.
3
FESEM Imaging of Gold-Coated Samples
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SEM images were taken with an FESEM 7600 F (JEOL, Japan). Samples were sputter-coated with gold at a thickness of 20 nm using a JFC-1600 (JEOL, Japan) (20 mA, 80 s), and images were recorded with an acceleration voltage of 5.00 kV at different magnifications to provide morphological information.
4
Morphological Characterization of ICC Hydrogels
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ICCs morphology was observed with Scanning Electron Microscopy (SEM). For SEM analysis, hydrogels were fixed with paraformaldehyde (PFA; 4%, 10 min; Alfa Aesar), sequentially dehydrated with increasing concentrations of ethanol (25%, 50%, 75%, 95% and 100%; 30 min each), freezed (−80 °C, overnight) and freeze-dried (48 h; FreeZone 4.5 litre freeze-dryer; Labconco). Samples (top surface) were coated with a platinum (Pt) film (10 nm) in a JFC-1600 sputter coater (JEOL) (20 mA, 60 s) and images were acquired with a FESEM 7600F (JEOL; 5 kV, different magnifications).
Collagen- or fibronectin-coating of ICC hydrogels was evaluated by immunofluorescence. Briefly, Col-ICCs and FN-ICCs were fixed with PFA (4%, 10 min), washed with PBS and incubated (4 °C, overnight) with a mouse anti-collagen I primary antibody (1 : 100; Abcam) or a rabbit anti-fibronectin primary antibody (1 : 100; Abcam), respectively, in a bovine serum albumin (BSA; Sigma-Aldrich) solution (3% w/v in PBS). ICCs were then washed with PBS and incubated with a goat anti-mouse (Life Technologies) or a goat anti-rabbit (Life Technologies) secondary antibody conjugated with Alexa Fluor 488 (1 : 100; 2 h). Fluorescent images were acquired with an LSM710 confocal microscope (Carl Zeiss) equipped with an Axio Observer Z1 inverted microscope (Carl Zeiss). Reconstructed 3D images were obtained using ZEN software (Zeiss).
Collagen- or fibronectin-coating of ICC hydrogels was evaluated by immunofluorescence. Briefly, Col-ICCs and FN-ICCs were fixed with PFA (4%, 10 min), washed with PBS and incubated (4 °C, overnight) with a mouse anti-collagen I primary antibody (1 : 100; Abcam) or a rabbit anti-fibronectin primary antibody (1 : 100; Abcam), respectively, in a bovine serum albumin (BSA; Sigma-Aldrich) solution (3% w/v in PBS). ICCs were then washed with PBS and incubated with a goat anti-mouse (Life Technologies) or a goat anti-rabbit (Life Technologies) secondary antibody conjugated with Alexa Fluor 488 (1 : 100; 2 h). Fluorescent images were acquired with an LSM710 confocal microscope (Carl Zeiss) equipped with an Axio Observer Z1 inverted microscope (Carl Zeiss). Reconstructed 3D images were obtained using ZEN software (Zeiss).
5
Characterization of Synthesized Electrochemical Samples
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The morphology and structure of the synthesized and electrochemically tested samples were observed by SEM (JEOL, FESEM 7600F) and TEM (JEOL, JEM 3010, 300 kV). The samples were directly used for SEM observations. For TEM observations, the samples were firstly sonicated in ethanol and then transferred onto holey carbon membranes supported by copper grids. Raman spectroscopy (Renishaw, RM-1000 Invia) with an excitation energy of 2.41 eV (514 nm, Ar+ ion laser) was used to characterize the optical properties of the samples.
6
Characterizing Lipid-Coated Hollow Nanoparticles
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The hydrodynamic
diameters and zeta potentials (ζ-potential) of LCHNPs were measured
by dynamic light scattering using a Malvern Zeta Sizer ZS. Briefly,
lyophilized powder of LCHNPs was diluted in deionized H2O to obtain 1 mg/mL suspensions, which were then added into a Malvern
disposable folded capillary cell (DTS1070) and inserted into the equipment
for measurements to be taken. For FESEM, the sample was prepared by
applying a thin layer of LCHNP dry powder onto carbon tape. This was
followed by sputter-coating the layer with Pt for 15 s after which
the sample was then imaged under JEOL FESEM 7600 F at 2 kV acceleration
voltage with a probe current of 8. For TEM, freshly fabricated LCHNP
suspension was added onto lacey carbon copper grids for 2 min followed
by staining with 5 μL of UranyLess solution for 3 min. The resulting
sample was then imaged using JEOL 2010HR with an acceleration voltage
of 200 kV.
diameters and zeta potentials (ζ-potential) of LCHNPs were measured
by dynamic light scattering using a Malvern Zeta Sizer ZS. Briefly,
lyophilized powder of LCHNPs was diluted in deionized H2O to obtain 1 mg/mL suspensions, which were then added into a Malvern
disposable folded capillary cell (DTS1070) and inserted into the equipment
for measurements to be taken. For FESEM, the sample was prepared by
applying a thin layer of LCHNP dry powder onto carbon tape. This was
followed by sputter-coating the layer with Pt for 15 s after which
the sample was then imaged under JEOL FESEM 7600 F at 2 kV acceleration
voltage with a probe current of 8. For TEM, freshly fabricated LCHNP
suspension was added onto lacey carbon copper grids for 2 min followed
by staining with 5 μL of UranyLess solution for 3 min. The resulting
sample was then imaged using JEOL 2010HR with an acceleration voltage
of 200 kV.
7
SEM Imaging of Spores and SECs
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FESEM 7600F (JEOL, Japan) was used for SEM image processing. Cross section samples were prepared by mounting intact spores or SECs on SEM sample mounting tape, followed by immersion in liquid nitrogen for 30 s. A steel blade (No. 10 round edge carbon steel scalpel blade, RS Components, Singapore) was used to slice across the particles several times. The cut lines were followed during SEM until a suitable cracked particle was found. All samples were coated with platinum at a thickness of 10 nm using JFC-1600 (JEOL, Japan) (20 mA, 60 sec). Images were recorded with an acceleration voltage of 5.00 kV at different magnifications to observe morphological changes before and after each SEC extraction processing step.
8
Validating Silica-Coated ZnO NPs
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EDX analysis was performed to validate the formation of a silica coat on the ZnO NP surface. 2 mg of ZnO or Si-ZnO NP dry powder was mounted on carbon tape and adhered to the sample holder. The sample-containing holder was then loaded into a scanning electron microscope (JEOL FE-SEM 7600F) and analyzed using an integrated EDX analysis system from Oxford instruments (UK).
9
Cell Morphology and Spreading on Scaffolds
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The cell morphology and the cell spreading pattern interaction of hPDL mesenchymal stromal cells seeded at 1×10 4 cells/mL onto films and PLA nanofiber scaffolds were examined by using field emission scanning electron microscopy (FE-SEM 7600F, JEOL) after 24 h of cell culture. At the end of the incubation time, scaffolds were washed three times with PBS, fixed with 4% formaldehyde for 1 h and then dehydrated with a graded series of ethanol (25-100%) and air dried. The samples were sputter-coated with a thin layer of gold-palladium and examined by FE-SEM.
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