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Pdha1

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PDHA1 is a protein that is a component of the pyruvate dehydrogenase complex, which plays a key role in cellular energy metabolism by converting pyruvate to acetyl-CoA. This protein is essential for the oxidative decarboxylation of pyruvate, a critical step in the tricarboxylic acid cycle.

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Lab products found in correlation

Ampkα, by Cell Signaling Technology (2 mentions) β actin, by Cell Signaling Technology (2 mentions) Phospho ampkα thr172, by Cell Signaling Technology (1 mentions) Hif 1α antibody, by Abcam (1 mentions) Vimentin antibody, by Proteintech (1 mentions) Hsp60 antibody, by Stressgen (1 mentions) β catenin, by Merck Group (1 mentions) N cadherin, by Cell Signaling Technology (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Ab155138, by Abcam (1 mentions) Cyt c, by Cell Signaling Technology (1 mentions) Paccs79, by Cell Signaling Technology (1 mentions) Gsk3α β, by Cell Signaling Technology (1 mentions) Sirt3, by Cell Signaling Technology (1 mentions) P ampkαt172, by Cell Signaling Technology (1 mentions) Pakt t308, by Cell Signaling Technology (1 mentions) Pkm1 2, by Cell Signaling Technology (1 mentions) Pgsk3α β s21 s9, by Cell Signaling Technology (1 mentions) Glut4, by Santa Cruz Biotechnology (1 mentions) Pgc 1α, by Merck Group (1 mentions)

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Anti-PDHA1 (3 citations)

3 protocols using pdha1

1

Western Blot Analysis of Cellular Proteins

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Cells were lysed in lysis buffer (20 mmol/L Tris-HCl pH 7.5, 150 mmol/L NaCl, 1% Triton X-100, 1% sodium pyrophosphate, Protease Inhibitor Cocktail) for 30 min on ice. The supernatant was collected after centrifugation at 14,000×g for 20 min at 4°C. Protein concentrations were determined using the BCA protein assay kit. Proteins were separated on the 12% SDS-PAGE gel and transferred onto a PVDF transfer membrane. Western blot analysis followed a standard procedure. NDUFA4, ENO2, MCT1 and β-catenin antibodies were obtained from Sigma (St Louis, MO). AMPKα, phospho-AMPKα (Thr172), PDHA1, E-cadherin, N-cadherin and β-actin were obtained from Cell Signaling Technology (Danvers, MA). The antibody for cytokeratin 18 was obtained from Millipore (Boston, MA). HIF1α antibody was obtained from Abcam (Cambridge, MA). Vimentin antibody was obtained from Proteintech (Chicago, IL). NDUFA5 and ADHFE1 antibodies were obtained from Pierce (Rockford, IL). HSP60 antibody was obtained from Stressgen (Victoria, BC).
Tang H., Chen Y., Liu X., Wang S., Lv Y., Wu D., Wang Q., Luo M, & Deng H. (2016). Downregulation of HSP60 disrupts mitochondrial proteostasis to promote tumorigenesis and progression in clear cell renal cell carcinoma. Oncotarget, 7(25), 38822-38834.
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2

Protein Expression Analysis in Cells

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Equal amounts of proteins were separated using SDS-PAGE under reducing conditions. Proteins of interest were detected using the following antibodies; SIRT1 (S5196, Sigma-Aldrich), SIRT3 (5490S, Cell signaling), SIRT6 (GTX105611, Gene Tex), GLUT4 (sc53566, Santa Cruz Biotechnology), HK2 (2857, Cell signaling), PKM1/2 (3190, Cell signaling), PDHA1 (3205, Cell signaling), ACADVL (ab155138, Abcam), ACADL (ab82853, Abcam), CD36 (ABM-5525, Cascade Bioscience), AMPKα (G. Hardie, University of Dundee, Dundee, Scotland, UK), pAMPKα T172 (2531, Cell signaling), ACC (3661, Cell signaling), pACC S79 (3662, Cell signaling), PGC-1α (3242, EMD Millipore), ATP5A, UQCRC2, MTCO1, SDHB, NDUFB8 (MS604, MitoSciences), COX4 (4850P, Cell signaling), CYTC (11940, Cell signaling), eEF2 (2332, Cell signaling), Akt2 (2964, Cell signaling), pAkt S473 (9271, Cell signaling), pAkt T308 (9275, Cell signaling), GSK3α/β (5676, Cell signaling), pGSK3α/β S21/S9 (9331, Cell signaling). Densitometric analysis of immunoblots was performed on four or seven individual samples using Image Lab Software (Bio-Rad Laboratories), and a representative selection is presented in each figure.
Svensson K., LaBarge S.A., Martins V.F, & Schenk S. (2017). Temporal overexpression of SIRT1 in skeletal muscle of adult mice does not improve insulin sensitivity or markers of mitochondrial biogenesis. Acta physiologica (Oxford, England), 221(3), 193-203.
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3

Comprehensive Protein Quantification and Immunoblotting

Cited 1 time
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Immunoblotting has been previously described (Messaggio et al. 2017 (link)). Protein samples were quantified using BCA (Pierce). 20–30 µg of protein were loaded per lane. Antibodies against pSHP2 [Y542 (cat# 3751)], SHP2 (cat# 3397), PDHA1 (cat# 3205), HK1 (cat# 2024), Lamin B1 (cat# 12586), p-AMPKα [Thr 172 (cat# 2535)], UCP1 (cat#14670), Perilipin-1 (cat# 9349), beta-actin (cat# 4970) and GAPDH (cat# 5174) were purchased from cell signaling technologies. Glut1 was from Invitrogen (cat# PA1-46152). Tubulin (cat# E7-s) was purchased from the Developmental Studies Hybridoma Bank (DSHB, Iowa City, IA).
Olou A.A., Ambrose J., Jack J.L., Walsh M., Ruckert M.T., Eades A.E., Bye B.A., Dandawate P, & VanSaun M.N. (2022). SHP2 regulates adipose maintenance and adipocyte-pancreatic cancer cell crosstalk via PDHA1. Journal of Cell Communication and Signaling, 17(3), 575-590.
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