2 mercaptobenzothiazole mbt

Manufactured by Merck Group

Sourced in Poland, United States

2-mercaptobenzothiazole (MBT) is a chemical compound used as a laboratory reagent. It is a crystalline solid with a characteristic odor. MBT is commonly employed in various analytical and research applications, where its specific properties may be utilized.

Automatically generated - may contain errors

Lab products found in correlation

10 protocols using 2 mercaptobenzothiazole mbt

Autoradiographic Binding Assay Reagents

Check if the same lab product or an alternative is used in the 5 most similar protocols

192IgG-saporin
(batch 2441969) was acquired
from Millipore (Temecula, CA, USA). RRID AB_94979, [³H]-pirenzepine
(86.0 Ci/mmol, catalog no. NET80250UC), [³H]-oxotremorine
(75.8 Ci/mmol, catalog no. NET671), and [³⁵S]GTPγS
(1250 Ci/mmol, catalog no. NEG030H250UC) were from PerkinElmer (Boston,
MA, USA). Both [³H]- and [¹⁴C]-microscales (catalog
no. ART0123A and catalog no. ARC0146, respectively) were used as standards
in the autoradiographic experiments, ARC (American Radiolabeled Chemicals,
Saint Louis, MO, USA). The β-radiation-sensitive Kodak Biomax
MR films (catalog no. 7358460), bovine serum albumin (BSA) (catalog
no. A4503), carbachol (catalog no. C4382), pirenzepine (catalog no.
P7412), oxotremorine (catalog no. O9126), atropine (catalog no. A0257)
scopolamine (catalog no. S0929), dl-dithiothreitol (DTT)
(catalog no. D5545), adenosine deaminase (ADA) (catalog no. A9876),
guanosine 5′-diphosphate (GDP) (catalog no. G7127), guanosine
5′-O-3-thiotriphosphate (GTPγS) (catalog
no. G8634), ketamine (catalog no. K2753), xylazine (catalog no. X1251),
acetylthiocholine iodide (catalog no. 01480), 2-mercaptobenzothiazole
(MBT) (catalog no. M3302), and tetraisopropyl pyrophosphoramide (iso-OMPA)
(catalog no. T1505) were all acquired from Sigma-Aldrich (St. Louis,
MO, USA).

Llorente-Ovejero A., Martínez-Gardeazabal J., Moreno-Rodríguez M., Lombardero L., González de San Román E., Manuel I., Giralt M.T, & Rodríguez-Puertas R. (2021). Specific Phospholipid Modulation by Muscarinic Signaling in a Rat Lesion Model of Alzheimer’s Disease. ACS Chemical Neuroscience, 12(12), 2167-2181.

+ Open protocol

+ Expand

G protein activation assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

[³⁵S]GTPγS (initial specific activity 1250 Ci/mmol) was purchased from Perkin Elmer (Boston MA, USA). Oleoyl-L-α-lysophosphatidic acid sodium salt was obtained from Sigma-Aldrich, (St. Louis, MO, USA), Ki16425 was purchased from Selleckchem (Houston, TX, USA), 2-mercaptobenzothiazole (MBT) was acquired from Sigma-Aldrich (St. Louis, MO, USA). The [¹⁴C]-microscales used as standards in the autoradiographic experiments were acquired from Amersham Biosciences-GE (Buckinghamshire, UK) and ARC (St. Louis, MO, USA), BSA (A4503), DL-dithiothreitol (DTT), guanosine-5′-diphosphate (GDP) and guanosine-5′-o-3-triphosphate were provided by Sigma (St. Louis, MO, USA) and the β-radiation sensitive films, Kodak Biomax MR, were supplied by Sigma. Finally, for the preparation of the different buffers, the treatment of slides and film development, all the compounds were of the highest commercially available quality.

González de San Román E., Manuel I., Giralt M., Chun J., Estivill-Torrús G., Rodriguez de Fonseca F., Santín L., Ferrer I, & Rodriguez-Puertas R. (2015). Anatomical Location of LPA1 Activation and LPA Phospholipid Precursors in Rodent and Human Brain. Journal of neurochemistry, 134(3), 471-485.

+ Open protocol

+ Expand

Sulfur Crosslinking of SBR Rubber

Check if the same lab product or an alternative is used in the 5 most similar protocols

SBR (KER1500) rubber with 23.5% bonded styrene and Mooney viscosity ML1 + 4 (100 °C): 50 was provided by Synthos SA (Oswiecim, Poland). It was crosslinked with sulfur in the presence of 2-mercaptobenzothiazole (MBT) or 1,3-diphenylguanidine (DPG) as accelerators (Sigma-Aldrich, Poznan, Poland). Zinc oxide characterized with a specific surface area of 10 m²/g (ZnO; Huta Bedzin, Będzin, Poland), together with stearic acid (St.A.; Sigma-Aldrich, Poznan, Poland), were applied as standard activators. As an additional activator/accelerator of sulfur vulcanization, Activ8 was used in the form of Premix Acti8 containing 50% active substance (provided by Torimex-Chemicals, Konstantynów Łódzki, Poland) mixed with silica and predispersed on a poly(ethylene oxide) (PEO) carrier. Activ8 material is a functionalized PEO terminated with silicate compound to provide its interaction with fillers. In addition, Activ8 contains sodium dibenzyldithiocarbamate, which ensures its accelerating activity in the vulcanization process [13 ]. Carbon black N550, provided by Konimpex Chemicals (Konin, Poland), and fumed silica Aerosil 380 (Evonik Industries, Essen, Germany) were applied as fillers. The structures of two main active ingredients of Activ8 are presented in Scheme 1.

Maciejewska M, & Siwek M. (2020). The Influence of Curing Systems on the Cure Characteristics and Physical Properties of Styrene–Butadiene Elastomer. Materials, 13(23), 5329.

+ Open protocol

+ Expand

Bioadditive-Enhanced Rubber Composite Synthesis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Field horsetail (HT) was delivered by MANU JTC, s.r.o. (Opawa, Poland). Common wheat (WS) was harvested from local farms. Hydrochloric acid (Sigma-Aldrich, Schnelldorf, Germany) and citric acid (Sigma-Aldrich, Schnelldorf, Germany) were used in the hydrolysis process. The polymer matrix was natural rubber (NR) supplied by Torimex-Chemicals Ltd. Sp. z.o.o (Konstatntynów Łódzki, Poland). The cross-linking system consisted of sulfur (Siarkopol, Tarnobrzeg, Poland), 2-mercaptobenzothiazole (MBT), purchased from Sigma-Aldrich (Schnelldorf, Germany), micrometric zinc oxide (ZnO, Huta Będzin SA, Będzin, Poland) and stearin (SA, Avantor Performance Materials, Gliwice, Poland).
The composition of the rubber mixture included (Table 1): natural rubber NR (100 phr-parts per hundred rubber), zinc oxide (5 phr), sulfur (2 phr), 2-mercaptobenzothiazole (2 phr), stearin (1 phr) and a filler (0 phr in in the case of the reference sample and 10 phr in the case of samples filled with the tested bioadditive).

Masłowski M., Miedzianowska J., Delekta M., Czylkowska A, & Strzelec K. (2021). Natural Rubber Biocomposites Filled with Phyto-Ashes Rich in Biogenic Silica Obtained from Wheat Straw and Field Horsetail. Polymers, 13(7), 1177.

+ Open protocol

+ Expand

Natural Rubber Composites with Ionic Liquids

Check if the same lab product or an alternative is used in the 5 most similar protocols

Natural rubber (NR, RSS1 type, cis-1,4-polyisoprene) with a density of 0.93–0.988 g/cm³ was obtained from Torimex Chemicals, Lodz, Poland. A conventional crosslinking system containing sulfur as a crosslinker (Siarkopol, Tarnobrzeg, Poland) in the presence of a vulcanization accelerator, i.e., 2-mercaptobenzothiazole (MBT), purchased from Sigma-Aldrich, Schelldorf, Germany, was applied. Zinc oxide (ZnO) with a specific surface area of 10 m²·g⁻¹ (Huta Bedzin, Bedzin, Poland) together with stearic acid (St.A, Sigma-Aldrich, Schelldorf, Germany) were used to activate the vulcanization. Silica AEROSIL^® 380 (A380) supplied by Evonik Industries (Essen, Germany) was used as a filler. Additionally, eight ionic liquids (ILs) with characteristics given in Table 1 were applied to enhance the vulcanization and properties of NR composites. ILs were provided by IoLiTec Ionic Liquids Technologies GmbH, Heilbronn, Germany. These ILs consist of bromide or chloride anion and different cations with butyl or decyl substituents. The general structures of ILs cations are given in Figure 1.

Maciejewska M, & Sowińska-Baranowska A. (2022). Bromide and Chloride Ionic Liquids Applied to Enhance the Vulcanization and Performance of Natural Rubber Biocomposites Filled with Nanosized Silica. Nanomaterials, 12(7), 1209.

+ Open protocol

+ Expand

Rubber Modification Using Oak Bark Filler

Check if the same lab product or an alternative is used in the 5 most similar protocols

In this work, natural rubber (NR, RSS I) from Torimex-Chemicals Ltd. Sp. z o. o. (Konstantynów Łódzki, Poland) was used as the elastomeric matrix. To cure the elastomer, the following components were used:

sulfur (S₈) as a cross-linking agent with a density of 1.8–2.36 g/cm³, supplied by Chempur (Piekary Śląskie, Poland),

zinc oxide (ZnO) as a cross-linking activator with a density of 5.6 g/cm³, obtained from Chempur (Piekary Śląskie, Poland),

2-mercaptobenzothiazole (MBT) as a cross-linking accelerator with a density of 1.29 g/cm³, delivered from Sigma-Aldrich (St. Louis, MO, USA),

stearic acid (SA), functioning as a cross-linking activator and dispersing agent with a density of 0.94 g/cm³, obtained from Chempur (Piekary Śląskie, Poland).

Oak bark (Quercus cortex) was used as a bio-filler, sourced from HerbaNordPol Ltd. Sp. z o. o. (Nowy Staw, Poland). For modification of the bio-filler, n-octadecyltrimethoxysilane with a density of 0.88 g/cm³ was employed, supplied by abcr GmbH (Karlsruhe, Germany).
As solvents to study the degree of cross-linking, the following substances were used:

toluene with a density of 0.87 g/cm³, delivered by POCh S.A. (Gliwice, Poland),

diethyl ether with a density of 0.71 g/cm³, obtained from Chempur (Piekary Śląskie, Poland).

Smejda-Krzewicka A., Mrozowski K, & Strzelec K. (2024). Effect of Modified and Unmodified Oak Bark (Quercus Cortex) on the Cross-Linking Process and Mechanical, Anti-Aging, and Hydrophobic Properties of Biocomposites Produced from Natural Rubber (NR). Materials, 17(9), 1968.

+ Open protocol

+ Expand

Determination of Benzomercaptans in Water

Check if the same lab product or an alternative is used in the 5 most similar protocols

Zinc nitrate hexahydrate, 2-methylimidazole (Hmim), n-butylamine (BA), ethylamine and hexylamine were purchased from Sigma-Aldrich (Milwaukee, WI, USA). Sodium hydroxide was obtained from Panreac Química SA (Barcelona, Spain). Acetonitrile (ACN), methanol (MeOH), acetic acid (HAcO) and hydrochloric acid were from VWR International Eurolab (Barcelona, Spain). The benzomercaptans selected in this work were: 2-mercaptobenzoxazole (MBO), 2-mercaptobenzothiazole (MBT) and 6nitrobenzo[d]thiazole-2-thiol (NMBT), all purchased from Sigma-Aldrich. The structures of these compounds are given in Table S1. Deionized water was prepared in Crystal B30 EDI Adrona deionizer (Riga, Latvia). All other reagents were of analytical grade unless otherwise stated. 1 mL empty propylene disposable SPE cartridge and frits (1/16′, 20 μm) were from Análisis Vínicos (Tomelloso, Spain). Individual standard solutions of benzomercaptans (1000 mg L -1 ) were prepared in MeOH and stored at 4 ºC until its use.
Working standard solutions were done daily by appropriate dilution from these stock solutions.

Martínez-Pérez-Cejuela H., Mompó-Roselló Ó., Crespí-Sánchez N., Palomino Cabello C., Catalá-Icardo M., Simó-Alfonso E.F, & Herrero-Martínez J.M. (2020). Determination of benzomercaptans in environmental complex samples by combining zeolitic imidazolate framework-8-based solid-phase extraction and high-performance liquid chromatography with UV detection. Journal of chromatography. A, 1631.

+ Open protocol

+ Expand

Radioligand Binding Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

[³⁵S]GTPγS (initial specific activity 1250 Ci/mmol) and [³H]CP55,940 (initial specific activity 144 Ci/mmol) were purchased from Perkin Elmer (Boston, MA, USA), Oleoyl-L-α-lysophosphatidic acid sodium salt was obtained from Sigma-Aldrich (St. Louis, MO, USA), WIN55,212-2 was purchased from Tocris, 2-mercaptobenzothiazole (MBT) was acquired from Sigma-Aldrich (St. Louis, MO, USA). The [¹⁴C]-microscales used as standards in the autoradiographic experiments were purchased from Amersham Biosciences (St. Louis, MO, USA). Moreover, DL-dithiothreitol (DTT), guanosine-5′-diphosphate (GDP) and guanosine-5′-o-3-trisphosphate were provided from Sigma (St. Louis, MO, USA), the β-sensitive films Kodak Biomax MR were supplied from Sigma (St. Louis, MO, USA). Finally, for the preparation of the incubation buffers, the treatment of slides, re-crystallization of the matrix and films developing, several different compounds supplied from different companies were used, and all the compounds were of the highest commercially available quality for the necessity of the neurochemical studies.

González de San Román E., Llorente-Ovejero A., Martínez-Gardeazabal J., Moreno-Rodríguez M., Giménez-Llort L., Manuel I, & Rodríguez-Puertas R. (2021). Modulation of Neurolipid Signaling and Specific Lipid Species in the Triple Transgenic Mouse Model of Alzheimer’s Disease. International Journal of Molecular Sciences, 22(22), 12256.

+ Open protocol

+ Expand

Curing Natural Rubber with Sulfur-Based System

Check if the same lab product or an alternative is used in the 5 most similar protocols

Natural rubber (NR) RSS I was obtained from Torimex-Chemicals Ltd. Sp. z o.o (Konstatntynów Łódzki, Poland). Rubber mixtures were cured using a conventional curing system containing sulfur (S) (Siarkopol, Tarnobrzeg, Poland) in the presence of zinc oxide (ZnO) (Sigma-Aldrich, St. Louis, MO, USA), stearic acid (SA) (Avantor Performance Materials, Gliwice, Poland), and 2-mercaptobenzothiazole (MBT) (Sigma-Aldrich, St. Louis, MO, USA).

Masłowski M., Miedzianowska J., Czylkowska A, & Strzelec K. (2020). Horsetail (Equisetum Arvense) as a Functional Filler for Natural Rubber Biocomposites. Materials, 13(11), 2526.

+ Open protocol

+ Expand

EPDM Rubber Compound Formulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

EPDM rubber (KEP330) was purchased from KUMHO Polychem., Daejeon, Korea. Slide-ring material (SH2400P) was procured from Advanced Soft Materials Inc. (Tokyo, Japan). Reagent grade -zinc oxide (>99.0% assay) and stearic acid were obtained from Daejung Chem., Seoul, Korea. Sulfur (99.5%) of 325 mesh size was supplied by Thermo-Fisher Scientific, Daejeon, Korea. Other compounding ingredients, such as 2-mercaptobenzothiazole (MBT) and tetramethyl thiuram disulfide (TMTD), were purchased from Sigma-Aldrich, Seoul, Korea and used as received.

Kim G., Sahu P, & Oh J.S. (2022). Physical Properties of Slide-Ring Material Reinforced Ethylene Propylene Diene Rubber Composites. Polymers, 14(10), 2121.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!