U2932

Manufactured by Leibniz Institute DSMZ

Sourced in Germany

The U2932 is a laboratory equipment designed for the cultivation and analysis of microorganisms. It provides a controlled environment for the growth and study of various microbial species. The core function of the U2932 is to maintain temperature, humidity, and other environmental conditions suitable for microbial cultures.

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Lab products found in correlation

14 protocols using u2932

Culturing Burkitt's and DLBCL Cell Lines

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Burkitt's lymphoma‐derived cell line Daudi was cultured as described previously.46 The DLBCL‐derived cell lines, SUDHL‐6, OCI‐Ly3 and U2932, were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ), and cultured according to the instructions. The mycoplasma test was performed using a PCR detection kit (TaKaRa‐Bio, Shiga, Japan).

Noyori O., Komohara Y., Nasser H., Hiyoshi M., Ma C., Pan C., Carreras J., Nakamura N., Sato A., Ando K., Okuno Y., Nosaka K., Matsuoka M, & Suzu S. (2019). Expression of IL‐34 correlates with macrophage infiltration and prognosis of diffuse large B‐cell lymphoma. Clinical & Translational Immunology, 8(8), e1074.

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Cell Culture of Lymphoma Lines

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Jeko-1, Mino, Daudi, U-2932, Raji, and Ramos cell lines were obtained from the German Collection of Microorganisms and Cell Cultures or the American Type Culture Collection and cultured in standard medium.

Her J.H., Pretscher D., Patra-Kneuer M., Schanzer J., Cho S.Y., Hwang Y.K., Hoeres T., Boxhammer R., Heitmueller C., Wilhelm M., Steidl S, & Endell J. (2022). Tafasitamab mediates killing of B-cell non-Hodgkin’s lymphoma in combination with γδ T cell or allogeneic NK cell therapy. Cancer Immunology, Immunotherapy, 71(11), 2829-2836.

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Sourcing and Culturing DLBCL Cell Lines

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Fresh-frozen biopsies of histologically confirmed DLBCL samples were identified in the pathology archive at Leiden University Medical Center (LUMC). The study was approved by the Scientific Review Committee of the LUMC Department of Hematology under an applicable waiver of consent by the LUMC Ethical Committee (B16.048).
DLBCL cell lines OCI-Ly3 (RRID:CVCL_8800), TMD8 (RRID:CVCL_A442), and U2932 (RRID:CVCL_1896) were obtained from the German Collection of Microorganisms and Cell Cultures. Phoenix cells (RRID:CVCL_H717) were cultured at 5% CO₂ in Iscove’s Modified Dulbecco’s Medium (IMDM; Lonza) supplemented with 10% fetal bovine serum (FBS; Bodinco), 3 mM L-glutamine (Lonza), 100 U/ml penicillin/streptomycin (Lonza). TKO cells were used, which are generated from the Oct cell line (RRID:CVCL_WN86) that originates from transformed murine pre-B cells from Slp65^−/− mice. The TKO cell line has a Rag2 and Lambda5 KO. These cells were cultured at 7% CO₂ in Iscove’s Basal Medium (Merck) supplied with 5% FBS (PAN BioTech), 3 mM L-glutamine, 100 U/ml penicillin/streptomycin, 50 mM 2-mercaptoethanol (Sigma-Aldrich; Merck), and IL-7–containing supernatant of mouse plasmacytoma cells J558L (RRID:CVCL_3949), stably transfected with a murine IL-7 expression vector (Meixlsperger et al., 2007 (link)).

Eken J.A., Koning M.T., Kupcova K., Sepúlveda Yáñez J.H., de Groen R.A., Quinten E., Janssen J., van Bergen C.A., Vermaat J.S., Cleven A., Navarrete M.A., Ylstra B., de Jong D., Havranek O., Jumaa H, & Veelken H. (2024). Antigen-independent, autonomous B cell receptor signaling drives activated B cell DLBCL. The Journal of Experimental Medicine, 221(5), e20230941.

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B-cell Lymphoma Cell Line Cultures

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The following human B-cell lymphoma cell lines were used for culture: three Burkitt's lymphoma cell lines (BJAB, Raji, Daudi), one germinal center type DLBCL line (Toledo), and three activated B-cell type DLBCL cell lines (Riva, U2932, and OCI-Ly10). Toledo, Daudi and Raji were purchased from ATCC (Rockville, MD), and Riva and U2932 from DSMZ (Braunschweig, Germany). BJAB cells were obtained from Dr. W.S. Kim (Sungkyunkwan University) and OCI-Ly10 from Dr. Y.K. Jeon (Seoul National University Hospital). Patient-derived primary cells were acquired from malignant pleural effusion or ascites of patients with DLBCL. Seven patients had refractory disease whereas one patient achieved complete response to the 1^st line chemotherapy. Cells were cultured in RPMI 1640 supplemented with heat-inactivated 10% fetal bovine serum, penicillin and streptomycin (Gibco BRL, Grand Island, NY), except OCI-Ly10, which was cultured in Iscove's modified Dulbecco's medium.

Ryu K.J., Park C., Hong M., Ko Y.H., Kim W.S, & Kim S.J. (2016). FOXO4 expression is related to stem cell-like properties and resistance to treatment in diffuse large B-cell lymphoma. Oncotarget, 8(2), 2466-2476.

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Cell Line Maintenance and Validation

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RI-1, also known as RIVA, U2932, NU-DHL-1 cell lines were purchased from DSMZ. SU-DHL-4, SU-DHL-6 were purchased from ATCC. All lines were cultured in RPMI 1640 with L-Glutamine (ThermoFisher),10% fetal bovine serum (Thermofisher), at 37 degrees Celsius, 5% CO₂. OCI-LY10 cells, obtained from University Health Network, Ontario, Canada, were cultured in Iscove’s Modified Dulbecco’s medium (Thermofisher) with 20% human serum (Valley Biomedical). IMPACT III testing (IDEXX Bioresearch) was carried out on cell lines to confirm mycoplasma- and pathogen-free status. Cell lines were expanded and cryopreserved following 2–3 passages after obtaining from vendor. After thawing, cells were maintained (for experiments, xenografting) for a maximum of 8 weeks by sub-culturing approximately 3 times a week.

Dey J., Deckwerth T.L., Kerwin W.S., Casalini J.R., Merrell A.J., Grenley M.O., Burns C., Ditzler S.H., Dixon C.P., Beirne E., Gillespie K.C., Kleinman E.F, & Klinghoffer R.A. (2017). Voruciclib, a clinical stage oral CDK9 inhibitor, represses MCL-1 and sensitizes high-risk Diffuse Large B-cell Lymphoma to BCL2 inhibition. Scientific Reports, 7, 18007.

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Characterization of B-cell Lymphoma Lines

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For studies performed at ImmunoGen, the U-2932, SU-DHL-4, DOHH-2, OCI-Ly18, and OCI-Ly7 lines were obtained from DSMZ; the Farage cell line was purchased from ATCC. All lines were characterized by the vendor using routine DNA profiling; no further authentication was performed by the authors. Cells were expanded and maintained as recommended in a humidified 37 °C, 5% CO₂ incubator. All cell lines show membrane expression of both CD20 and CD37.

Hicks S.W., Lai K.C., Gavrilescu L.C., Yi Y., Sikka S., Shah P., Kelly M.E., Lee J., Lanieri L., Ponte J.F., Sloss C.M, & Romanelli A. (2017). The Antitumor Activity of IMGN529, a CD37-Targeting Antibody-Drug Conjugate, Is Potentiated by Rituximab in Non-Hodgkin Lymphoma Models. Neoplasia (New York, N.Y.), 19(9), 661-671.

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Cell Lines and Primary Cell Isolation

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Jurkat E6.1 T lymphocytes, Hela, HEK293T and MCF7 cells were purchased from American Type Culture Collection (ATCC). U2932, SUDHL4, BJAB, RIVA, OCI-LY3, and OCI-LY19 cells were acquired from DSMZ. HBL1, HLY1 and TMD8 cells were kindly provided by Martin Dyer, Pierre Brousset, and Daniel Krappmann, respectively. Blood was obtained from healthy donors (Etablissement Français du Sang). Peripheral blood mononuclear cells (PBMC) were acquired by Ficoll density gradient. Primary CD4 + and CD8 + T cells were subsequentially isolated from PBMC using REAlease CD4 and CD8 microbeads kits, from Miltenyi as manufacturer's instructions. Cell viability was assessed using CellTiter-Glo following manufacturer's instructions (Promega) following stimulation with 10 ng/mL TNFα (R&D systems) for 24h.

Thys A., Trillet K., Rosińska S., Gayraud A., Douanne T., Danger Y., Renaud C., Antigny L., Lavigne R., Pineau C., Com E., Vérité F., Gavard J., & Bidère N. (2020). Serine 165 Phosphorylation of SHARPIN regulates the Activation of NF-κB.

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Culturing ABC-type DLBCL Cell Lines

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ABC-type DLBCL cell lines, U2932, OCI-LY3, and RCK8 were purchased from DSMZ and were grown in a RPMI1640 medium supplemented with 10% FBS without antibiotics and kept in a 37°C, 5% CO₂ incubator. All cell lines were Mycoplasma free tested with MycoAlert mycoplasma detection kit (LONZA). ABC-type DLBCL cell lines were chosen because of their high miR-155 expression, compared with normal CD19⁺ B cells.

Anastasiadou E., Seto A.G., Beatty X., Hermreck M., Gilles M.E., Stroopinsky D., Pinter-Brown L.C., Pestano L., Marchese C., Avigan D., Trivedi P., Escolar D.M., Jackson A.L, & Slack F.J. (2020). Cobomarsen, an Oligonucleotide Inhibitor of miR-155, Slows DLBCL Tumor Cell Growth In Vitro and In Vivo. Clinical cancer research : an official journal of the American Association for Cancer Research, 27(4), 1139-1149.

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Anticancer Cell Line Panel Evaluation

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Four human cancer cell lines were used, comprising two different cell lines that are sensitive to each of the anticancer agents used, as follows: VCR or OXP. The sensitivity of each cancer cell line to chemotherapy agents was based on the literature, the DepMap, and the “Genomics of drugs and sensitivity of cancers” (GDSC) databases. The cell lines used are as follows:

Two diffuse large B-cell lymphoma cell lines, SU-DHL-4 (ATCC^® CRL-2957™, ATCC, Manassas, VA, USA) and U-2932 (DSMZ^® ACC633), sensitive to VCR (suspension cell culture).

Two colorectal cancer cell lines, HT29 (ATCC^® HTB-38™) and HCT116 (ATCC^® CCL-247™), sensitive to OXP (adherent cell culture),

Cell lines were cultured in the medium recommended by ATCC^®. Culture media were supplemented with 10% heat-inactivated fetal bovine serum, 1% L-glutamine, 1% non-essential amino acids, and 1% penicillin/streptomycin (Gibco, Waltham, MA, USA). All cells were grown at 37 °C in a humidified air atmosphere containing 5% CO₂.

Mroué M., Bessaguet F., Nizou A., Richard L., Sturtz F., Magy L., Bourthoumieu S., Danigo A, & Demiot C. (2024). Neuroprotective Effect of Polyvalent Immunoglobulins on Mouse Models of Chemotherapy-Induced Peripheral Neuropathy. Pharmaceutics, 16(1), 139.

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Cell Culture Protocol for Lymphoma Lines

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The human cell lines, BL-41, GRANTA-519, DAUDI, REC-1, SU-DHL-4, U2932, SC-1, MINO, K562, and MAVER-1 were obtained from DSMZ. J76 was a kind gift from M. Heemskerk (Leiden University Medical Center, the Netherlands). EBV-transformed B cell lines were generated in house. The cells were cultured in RPMI 1640 (PAA, Paschung, Austria) and supplemented with 10% fetal calf serum (FCS, PAA) and 50 μg/ml Gentamycin (Thermo Fischer, Oslo, Norway). The Phoenix-AMPHO (CRL-3213) cell line was purchased from ATCC (Manassas, VA, USA) and was maintained in Dulbecco's modified Eagle's medium (Sigma-Aldrich, Oslo, Norway) supplemented with 10% FCS and 50 μg/ml Gentamycin.

Köksal H., Dillard P., Juzeniene A., Kvalheim G., Smeland E.B., Myklebust J.H., Inderberg E.M, & Wälchli S. (2020). Combinatorial CAR design improves target restriction. The Journal of Biological Chemistry, 296, 100116.

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