Reverse transcription and real time quantitative polymerase chain reaction rt qpcr kits

Nicotine (CAS No. 54-11-5) and caffeine (CAS No. C0750) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethanol was obtained from Zhen Xin Co., Ltd. (Shanghai, China). The glucose colorimetric detection kit was purchased from Shanghai Rongsheng Biotech Co., Ltd. (Shanghai, China). The rat insulin enzyme linked immuno sorbent assay (ELISA) kit was provided by Mercodia (Uppsala, Sweden). The rat testosterone enzyme ELISA kit was provided by North Institute of Biological Technology (Beijing, China). The corticosterone (CORT) assay kit was purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Total cholesterol (T-CHO), triglyceride (TG), low-density lipoprotein cholesterol (LDL-c), and high-density lipoprotein cholesterol (HDL-c) commercial kits were bought from Jiancheng Bioengineering Institute (Nanjing, China). TRIzol reagent was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Reverse transcription and real-time quantitative polymerase chain reaction (RT-qPCR) kits were purchased from Takara Biotechnology (Dalian, China). Fetal bovine serum (FBS) was purchased from Gibco (Grand Island, NY, USA). All other reagents were of analytical grade.

Ethanol (analytical pure grade) was purchased from Zhen Xin Co., Ltd. (Shanghai, China). Isoflurane was obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Ultrasensitive Rat insulin ELISA kits, Rat insulin ELISA kits and Rat/Mouse Proinsulin ELISA kits were provided by Mercodia (Uppsala, Sweden). Glucose oxidase assay kits were purchased from Mind Bioengineering Co. Ltd. (Shanghai, China). TRIzol reagent was purchased from Invitrogen Co. (Carlsbad, CA, USA). Reverse transcription and real-time quantitative polymerase chain reaction (RT-qPCR) kits were offered by TaKaRa Biotechnology Co., Ltd. (Dalian, China). Oligonucleotide primers for rat RT-qPCR genes (PAGE purification) were custom synthesized by Sangon Biotech Co., Ltd. (Shanghai, China). RNAlater was provided by Qiagen (Düsseldorf, North Rhine-Westphalia, Germany). Glucocorticoid receptor (GR), IGF1, and insulin gene enhancer protein isl1 (ISL1) antibodies were obtained from Abcam (San Francisco, California, USA). Insulin antibody was provided by Sigma-Aldrich (St. Louis, Missouri, USA). Immunohistochemical staining agents were purchased from Zhongshan Golden Bridge Biotechnology Co. Ltd. (Beijing, China).