Vario micro cube machine

Artesunic Acid (ARTA) and DMF were purchased from ABCR (Karlsruhe, Germany) and Sigma Aldrich (Taufkirchen, Germany) (95 % pure). Betulinic Acid (BETA) was purchased from ABCR (Karlsruhe, Germany) (90 % pure), additionally this compound was purified via column chromatography to achieve >98 % purity (Figure 5).
Thin layer chromatography (TLC) was performed on pre-coated aluminum sheets ALUGRAM^® SIL G/UV254 (0.2 mm silica gel with fluorescent indicator, MachereyNagel & Co). ¹H-NMR (¹³C-NMR) spectra were recorded at room temperature on a Bruker Avance spectrometer operating at 300 MHz. All chemical shifts are given in the ppm-scale and refer to the nondeuterized proportion of the solvent. ESI mass spectrum was recorded on a Bruker Daltonik micrOTOF II focus TOF MS-spectrometer. Elemental Analysis (C, H, N), carried out with an Elementar vario MICRO cube machine, is within ±0.40% of the calculated values confirming a purity of >95%.
For our biological studies all drugs were solved under sterile conditions in dimethylsulfoxide (DMSO) to concentration of 20mM.

Detailed information about synthesis of hybrid compounds are given in the supplementary information. ¹H NMR and ¹³C NMR spectra were recorded at RT on a Bruker Avance spectrometer operating at 300, 500, and 600 MHz. ESI mass spectra were recorded on a Bruker micrOTOF II focus TOF MS-spectrometer. Elemental analysis (C, H, N), carried out with an Euro EA 3000 (Euro Vector) machine and an Elementar vario MICRO cube machine, calculated values confirm a purity of >95%. We obtained the crystals of SARB hybrid in solvent acetonitrile and molecular structure was unambiguously determined by X-ray crystallography (Fig. 1). The data for the crystal structure of SARB hybrid can be found free of charge in: The Cambridge Crystallographic Data Centre (via www.cam.ac.uk/data_request/cif with the deposition number CCDC-1856382).

Dihydroartemisinin (> 98% (HPLC)) was purchased from TCI Deutschland GmbH, and sulfasalazine (> 98% (HPLC)) was purchased from Sigma Aldrich, Germany. Thin layer chromatography (TLC) was performed on pre-coated aluminum sheets ALUGRAM® SIL G/UV254 (0.2 mm silica gel with fluorescent indicator, MachereyNagel & Co). NMR spectra were recorded at room temperature on a Bruker Avance spectrometer operating at 300 MHz. All chemical shifts are given in the ppm-scale and refer to the nondeuterized proportion of the solvent. ESI mass spectrum was recorded on a Bruker Daltonik micrOTOF II focus TOF MS-spectrometer. Elemental Analysis (C, H, N), carried out with an Elementar vario MICRO cube machine, is within ± 0.40% of the calculated values confirming a purity of > 95%.