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Unity plus 400

Manufactured by Agilent Technologies
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The Unity-Plus-400 is a laboratory instrument designed for analytical applications. It provides accurate and reliable measurements, with core functionality to support various research and testing procedures. The detailed specifications and intended use cases for this product are not available within the scope of this request.

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Lab products found in correlation

Apex 2 mass spectrometer, by Bruker (5 mentions) Spherical c18 column, by Waters Corporation (5 mentions) Uv 240 spectrophotometer, by Jasco (5 mentions) Dip 370 polarimeter, by Jasco (5 mentions) Spherical c18 100a reversed phase silica gel rp 18, by Silicycle (5 mentions) 2000 ft ir spectrophotometer, by PerkinElmer (4 mentions) Mercury 500, by Agilent Technologies (3 mentions) Precoated silica gel 60 f254 plates, by Merck Group (3 mentions) Silica gel 60, by Merck Group (3 mentions) Vnmrs 600, by Agilent Technologies (2 mentions) Nicolet ir100 ftir spectrometer, by Thermo Fisher Scientific (2 mentions) F254 precoated plates, by Merck Group (1 mentions) Ftir spectrophotometer, by PerkinElmer (1 mentions) Vnmrs 700, by Agilent Technologies (1 mentions) Inova 500, by Agilent Technologies (1 mentions) Vnmrs 500, by Agilent Technologies (1 mentions) Spectrum bx ft ir spectrometer, by PerkinElmer (1 mentions) Mr 400, by Agilent Technologies (1 mentions) Mercury 400, by Agilent Technologies (1 mentions) X pert diffractometer, by Philips (1 mentions)

11 protocols using unity plus 400

1

Analytical Techniques for Chemical Characterization

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For the TLC, we used silica gel 60 F254 precoated plates (Merck); for column chromatography (CC), silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). For the HPLC, we used a spherical C18 column (250 × 10 mm, 5μm) (Waters) and LDC-Analytical-III apparatus. For the UV spectra, we used a Jasco UV-240 spectrophotometer, λmax (log ε) in nm. For the optical rotation, we used a Jasco DIP-370 polarimeter, in CHCl3. For the IR spectra, we used a Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm−1. For the 1H-, 13C- and 2D-NMR spectra, we used Varian-Mercury-500 and Varian-Unity-Plus-400 spectrometers; δ in ppm rel. to Me4Si, J in Hz. For the ESI and HRESIMS, we used a Bruker APEX-II mass spectrometer, in m/z.
Wu M.D, & Cheng M.J. (2022). Undescribed Metabolites from an Actinobacteria Acrocarpospora punica and Their Anti-Inflammatory Activity. Molecules, 27(22), 7982.
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2

Characterization of Organic Compounds by NMR and MS

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All chemicals were purchased from commercial suppliers and were used as received unless otherwise stated. Proton Nuclear Magnetic Resonance NMR (1H NMR) spectra and carbon nuclear magnetic resonance (13C NMR) spectra were recorded on a Varian Unity Plus 400, Varian MR400, Varian vnmrs 500, Varian Inova 500, Varian Mercury 500, and Varian vnmrs 700 spectrometers. Chemical shifts for protons are reported in parts per million and are references to the NMR solvent peak (CDCl3: δ 7.26). Chemical shifts for carbons are reported in parts per million and are referenced to the carbon resonances of the NMR solvent (CDCl3: δ 77.23). Data are represented as follows: chemical shift, multiplicity (br = broad, s = singlet, d = doublet, t = triplet, q = quartet, dq = doublet of quartet, ddq = doublet of doublet of quartet, p = pentet, dd = doublet of doublet, ddd = doublet of doublet of doublet, hept = heptet, m = multiplet), coupling constants in Hertz (Hz) and integration. Mass spectroscopic (MS) data was recorded at the Mass Spectrometry Facility at the Department of Chemistry of the University of Michigan in Ann Arbor, MI on an Agilent Q-TOF HPLC-MS with ESI high resolution mass spectrometer. Infrared (IR) spectra were obtained using either an Avatar 360 FT-IR or Perkin Elmer Spectrum BX FT-IR spectrometer. IR data are represented as frequency of absorption (cm−1).
McAtee C.C., Ellinwood D.C., McAtee R.C, & Schindler C.S. (2018). Functionalized Cyclopentanes via Sc(III)-Catalyzed Intramolecular Enolate Alkylation. Tetrahedron, 74(26), 3306-3313.
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3

Analytical Methods for Natural Product Research

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For the TLC, we used silica gel 60 F254-precoated plates (Merck); for column chromatography (CC), we used silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). For the HPLC analysis, we used a spherical C18 column (250 × 10 mm, 5 μm) (Waters) and LDC-Analytical-III apparatus. For the UV spectra, we used a Jasco UV-240 spectrophotometer, with λmax (log ε) in nm. For optical rotation, we used a Jasco DIP-370 polarimeter, in CHCl3. For the IR spectra, we used a Perkin-Elmer-2000 FT-IR spectrophotometer, with ν in cm−1. For the 1H-, 13C-, and 2D-NMR spectra, we used Varian-VNMRS-600 and Varian-Unity-Plus-400 spectrometers; δ in ppm relative to Me4Si, J in Hz. For the ESI and HRESIMS, we used a Bruker APEX-II mass spectrometer, in m/z.
Cheng M.J., Chen J.J., Wu M.D., Leu J.Y, & Tseng M. (2023). Antifungal Activities of Compounds Produced by Newly Isolated Acrocarpospora Strains. Antibiotics, 12(1), 95.
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4

Characterization of Thermoplastic Polyurethane

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The chemical structure and the quantitative composition of the TPUs were determined by proton nuclear magnetic resonance (1H-NMR). Approximately 10 mg of the samples were dissolved in 1 mL of deuterated DMSO (DMSO-d6). Heating was necessary to dissolve the samples, and in the case of white filaments (TPU-Ultimaker, FlexiSmart, PolyFlex_95A, Filaflex_95A) the solution was previously filtered to remove the inorganic white pigment. Spectra were acquired on a Unity Plus 400 instrument (Varian Inc, Palo Alto, CA, USA) at room temperature. All recorded spectra were referenced to the residual solvent signal at 2.50 ppm.
León-Calero M., Reyburn Valés S.C., Marcos-Fernández Á, & Rodríguez-Hernandez J. (2021). 3D Printing of Thermoplastic Elastomers: Role of the Chemical Composition and Printing Parameters in the Production of Parts with Controlled Energy Absorption and Damping Capacity. Polymers, 13(20), 3551.
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5

Analytical Techniques for Chemical Characterization

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TLC: silica gel 60 F254 precoated plates (Merck). Column chromatography (CC): silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). HPLC: Spherical C18 column (250 × 10 mm, 5μm) (Waters); LDC-Analytical-III apparatus. UV Spectra: Jasco UV-240 spectrophotometer; λmax (log ε) in nm. Optical rotation: Jasco DIP-370 polarimeter; in CHCl3. IR Spectra: Perkin-Elmer-2000 FT-IR spectrophotometer; ν in cm−1. 1H-, 13C- and 2D-NMR spectra: Varian-Mercury-400 and Varian-Unity-Plus-400 spectrometers; δ in ppm relative to Me4Si, J in Hz. ESI and HRESIMS: Bruker APEX-II mass spectrometer; in m/z.
Su Y.S., Chen J.J., Cheng M.J., Chai C.Y., Kwan A.L., Huang J.C, & Kuo Y.H. (2021). Saccharpiscinols A–C: Flavans with Potential Anti-Inflammatory Activities from One Actinobacteria Saccharomonospora piscinae. Molecules, 26(16), 4909.
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6

Synthesis and Characterization of Cerium-Based Catalyst

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All reagents for synthesis and analysis were commercially available from Aldrich and Merck Companies and were used without further purification. The infrared (IR) spectra were recorded using a Thermo Nicolet IR 100 FT-IR spectrometer. A field emission scanning electron microscope (FESEM), specifically the German-made ZEISS SIGMA VP model with a gold coating, was used to analyze the samples. Utilizing monochromatic Co-Kα (1.78897 Å) radiation and a Philips X’pert diffractometer, measurements of X-ray powder diffraction (XRD) were made. The N2 desorption/adsorption isotherms of the synthesized samples were obtained using the BET technique with a Microtrac Bel Corp Belsorp mini II instrument. The N2 adsorption isotherm at 77 K was measured using a Micromeritics ASAP 2030 surface area analyzer. Thermogravimetry (TGA) was performed to determine the thermal stability using an SDTQ600 V20.9 analyzer with a heating rate of 10 °C/min under airflow. The metal cerium ion loading of the catalyst was determined by inductively coupled plasma (ICP) analysis using a Vavian 715-ES instrument. Proton nuclear magnetic resonance (1H NMR) spectroscopy was conducted on a Varian UnityPlus 400 instrument.
Moghadaskhou F., Eivazzadeh-Keihan R., Sadat Z., Tadjarodi A, & Maleki A. (2023). Fabrication and characterization of a novel catalyst based on modified zirconium metal-organic-framework for synthesis of polyhydroquinolines. Scientific Reports, 13, 16584.
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7

Spectroscopic Characterization of Compounds

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1H and 13C NMR spectra were acquired on Varian Unity Plus 400 (Varian Inc, CA, USA) and Bruker Avance DRX 500 (Bruker, Switzerland) spectrometers using DMSO-d6 as a solvent and tetramethylsilane as an internal standard. Mass spectra were recorded on an LC–MS instrument with chemical ionization (CI). LC–MS data were recorded on an Agilent 1100 HPLC equipped with a diode-matrix and mass-selective detector Agilent LC/MSD SL. Column: Zorbax SB-C18, 4.6 mm × 15 mm. Eluent: A, acetonitrile–H2O with 0.1% of trifluoroacetic acid (TFA; 95:5); B, H2O with 0.1% of TFA. Flow rate: 1.8 mL/min. Thin layer chromatography (TLC) was detected on Polygram SIL G/UV254 plate (Machery-Nagel, Germany) using CHCl3–MeOH (19:1) as eluent. Column chromatography was performed using silica gel 60 (230–400 mesh, Merck, Germany) as the stationary phase. Melting points were determined using a Boetius melting point apparatus (Boetius Franz Kustner, Germany).
Muzychka L., Voronkina A., Kovalchuk V., Smolii O.B., Wysokowski M., Petrenko I., Youssef D.T., Ehrlich I, & Ehrlich H. (2021). Marine biomimetics: bromotyrosines loaded chitinous skeleton as source of antibacterial agents. Applied Physics. A, Materials Science & Processing, 127(1), 15.
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8

Analytical Methods for Compound Characterization

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For the TLC, we used silica gel 60 F254-precoated plates (Merck); for column chromatography (CC), we used silica gel 60 (70–230 or 230–400 mesh, Merck) and Spherical C18 100A Reversed Phase Silica Gel (RP-18) (particle size: 20–40 μm) (Silicycle). For the HPLC analysis, we used a spherical C18 column (250 mm × 10 mm, 5 μm) (Waters) and LDC-Analytical-III apparatus. For the UV spectra, we used a Jasco UV-240 spectrophotometer, with λmax (log ε) in nm. For optical rotation, we used a Jasco DIP-370 polarimeter, in CHCl3. For the IR spectra, we used a Perkin-Elmer-2000 FT-IR spectrophotometer, with ν in cm−1. For the 1H-, 13C-, and 2D-NMR spectra, we used Varian-VNMRS-600 and Varian-Unity-Plus-400 spectrometers; δ in ppm relative to Me4Si, J in Hz. For the ESI and HRESIMS, we used a Bruker APEX-II mass spectrometer, in m/z.
Wu M.D., Chen J.J, & Cheng M.J. (2023). Secondary Metabolites with Antifungal Activities from Mangrove Derived Fungus Monascus purpureus WMD2424. Marine Drugs, 21(4), 200.
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9

Synthesis of Quaternized Chitosan

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Chitosan was dispersed into the mixture of isopropanol and distilled water at 85°C. Glycidyl trimethyl ammonium chloride (GTMAC) was added in four portions at 2 h intervals. After stirring 8 h, the clear and yellowish reaction solution was poured into acetone. The reaction product was filtered, concentrated, and dried under vacuum at 40°C for 24 h to obtain the quaternized chitosan. The synthesis route is shown in Scheme 1. The degree of quaternization (DQ) was determined by conductometric titration of chloride ions using a standard AgNO3 solution in water. FT IR spectra were recorded by reflection method with a VECTOR 22 Fourier transform infrared spectrometer. 1H NMR spectra were obtained using a Varian UNITYplus-400 nuclear magnetic resonance.
Liu P., Meng W., Wang S., Sun Y, & Ashraf M.A. (2015). Quaternary ammonium salt of chitosan: preparation and antimicrobial property for paper. Open Medicine, 10(1), 473-478.
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10

Characterization of PAEs and HGPAE

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The chemical structure was characterized based on proton nuclear magnetic resonance spectra, which were recorded on a Varian UNITY Plus-400 nuclear magnetic resonance instrument (Palo Alto, CA, USA) using dimethyl sulfoxide as a solvent.
The buffering ability of PAEs and HGPAE was determined by acid/base titration. The details are as follows: the polymer solution was first adjusted to above pH 10 with 0.1 M NaOH and was then titrated with 0.1 M HCl. Titration profiles were plotted as changes in pH against the volume of HCl solution.
In addition, the pH sensitivity was tested by detecting the absorbance of HGPAE solutions at different pH values at 500 nm with UV spectrophotometry using a UV-2450 (Shimadzu, Kyoto, Japan).
Hu F., Wang H., Zhang S., Peng Y., Su L., Chang J, & Liu G. (2015). Inhibition of myeloid differentiation factor 88 signaling mediated by histidine-grafted poly(β-amino ester) ester nanovector induces donor-specific liver allograft tolerance. International Journal of Nanomedicine, 10, 4367-4382.
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