Cacl2

Manufactured by Kanto Chemical

Sourced in Japan

Calcium chloride (CaCl2) is a chemical compound that is commonly used in laboratory settings. It is a colorless, crystalline solid that is highly soluble in water. Calcium chloride is known for its ability to absorb moisture from the air, making it a useful desiccant.

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Lab products found in correlation

Sodium hydroxide (naoh), by Kanto Chemical (1 mentions) Cacl2, by Nacalai Tesque (1 mentions) Potassium chloride (kcl), by Nacalai Tesque (1 mentions) Rpmi 1640, by Fujifilm (1 mentions) Nahco3, by Nacalai Tesque (1 mentions) Mgcl2 6h2o, by Nacalai Tesque (1 mentions) K2hpo4 3h2o, by Nacalai Tesque (1 mentions) Na2so4, by Nacalai Tesque (1 mentions) Tris hydroxymethylaminomethane ch2oh 3cnh2, by Nacalai Tesque (1 mentions) 1 m hcl, by Nacalai Tesque (1 mentions) Nutrient broth, by Eiken Chemical (1 mentions) Deoxyribonuclease, by Nippon Gene (1 mentions) Mgso4, by Kanto Chemical (1 mentions) Hank s balanced salt solution, by Fujifilm (1 mentions) Human serum, by Merck Group (1 mentions) Sodium dodecyl sulfate (sds), by Fujifilm (1 mentions) Congo red, by Merck Group (1 mentions) Sodium chloride (nacl), by Fujifilm (1 mentions) Dithiothreitol (dtt), by Fujifilm (1 mentions) Lithium chloride (licl), by Fujifilm (1 mentions)

7 protocols using cacl2

Surface Modification and Characterization of Titanium Alloys

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A commercially pure, medical-grade Ti plate (ISO5832-2) (Ti > 99.5%) was provided by Nilaco Co., Tokyo, Japan. Ti-6Al-4V alloy plate (Ti = balance, Al = 6.18, V = 4.27 mass%) and Ti-15Zr-4Nb-4Ta alloy plate (Ti = balance, Zr = 14.51, Nb = 3.83, Ta = 3.94, Pd = 0.16, and O = 0.25 mass%) were supplied by Kobelco Research Institute, Inc., Hyogo, Japan.
The chemical reagents (NaOH, CaCl₂, ICl₃, ICl, NaI and povidone iodine (PVP-I)) used for surface treatment were reagent-grade and purchased from Kanto Chemical Co., Inc., Tokyo, Japan. Reagent-grade NaCl, NaHCO₃, KCl, K₂HPO₄·3H₂O, MgCl₂·6H₂O, CaCl₂, Na₂SO₄, tris-hydroxymethylaminomethane (CH₂OH)₃CNH₂, and 1 M HCl were purchased from Nacalai Tesque, Inc., Kyoto, Japan, and used for the preparation of simulated body fluid (SBF). Minimum essential medium (MEM) was obtained from Gibco, Thermo Fisher Scientific, Waltham, MA, USA, and used for the cell culture test. Nutrient broth (Eiken Chemical Co., Ltd. Tochigi, Japan) and RPMI 1640 (Fujifilm Wako Pure Chemical Corp., Osaka, Japan) were used for the antibacterial activity test.

Yamaguchi S., Le P.T., Shintani S.A., Takadama H., Ito M., Ferraris S, & Spriano S. (2021). Iodine-Loaded Calcium Titanate for Bone Repair with Sustainable Antibacterial Activity Prepared by Solution and Heat Treatment. Nanomaterials, 11(9), 2199.

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Primary Glial Cell Isolation Protocol

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Primary glial cells, mainly comprised of astrocytes and microglia, were obtained as previously described (36 ). Briefly, we isolated the cerebrum from postnatal day 2 mice of either sex in ice-cold Hanks’ balanced salt solution not containing Ca/Mg (FUJIFILM Wako Chemicals). The cerebrum was suspended in Hanks’ balanced salt solution containing 0.25% trypsin, 0.1 μl/ml deoxyribonuclease (Nippon Gene), 0.8 mM MgSO₄ (Kanto Chemical Co), and 1.85 mM CaCl₂ (Kanto Chemical Co) at 37 °C for 15 min. The obtained cell suspension was passed through a 100 μm cell strainer (Falcon) and centrifuged with a culture medium. The cell pellet was resuspended in the culture medium and seeded on cell culture plates. The culture medium was replaced at 4 days in vitro. The enrichment of glial cells was confirmed by immunocytochemistry using anti-glial fibrillary acidic protein and anti-Iba1 antibodies (Fig. S5).

Nagai-Ito Y., Xu L., Ito K., Kajihara Y., Ito G, & Tomita T. (2022). The atypical Rab GTPase associated with Parkinson’s disease, Rab29, is localized to membranes. The Journal of Biological Chemistry, 298(10), 102499.

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Chemical Compound Procurement for Biological Assays

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SDS, sorbitol, DTT, NaCl, and LiCl were purchased from Wako Pure Chemical Industries (Osaka, Japan). Fluconazole, voriconazole, hygromycin B, caffeine, and monensin sodium salt were purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Nourseothricin was purchased from Jena Bioscience (Dortmund, Germany). Congo red was purchased from Sigma-Aldrich (St. Louis, MO, USA). G418 was purchased from Enzo Life Science, Inc. (Farmingdale, NY, USA). CaCl₂ was purchased from Kanto Chemical Co., Inc. (Tokyo, Japan). Tunicamycin was purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Brefeldin A (BFA) was purchased from Funakoshi Co., Ltd. (Tokyo, Japan). Human serum [(from male AB clotted whole blood), USA origin, sterile-filtered] (Product ID: H6914) was purchased from Sigma-Aldrich (St. Louis, MO, USA).

Matsumoto Y., Yoshikawa A., Nagamachi T., Sugiyama Y., Yamada T, & Sugita T. (2022). A critical role of calcineurin in stress responses, hyphal formation, and virulence of the pathogenic fungus Trichosporon asahii. Scientific Reports, 12, 16126.

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Gelatin Zymography Analysis of MMP Activity

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Gelatin zymography was performed as previously described (49 ). Briefly, MRC-5V1 cells were transfected with the aforementioned pQCXIB vectors. Culture medium was changed to FBS-free DMEM 24 h post-transfection to allow conditioning for 24 h, after which conditioned medium was harvested and mixed with equal volumes of 2× sample buffer [0.125 M Tris–HCL, pH 6.8, 20% (v/v) glycerol, 4% (w/v) SDS, 0.005% (w/v) bromophenol blue (Sigma; B-6131)]. Samples were subjected to SDS-PAGE electrophoresis in 10% resolving gel containing 0.1% gelatin. After electrophoresis, proteins were renatured in freshly made 1× Zymogram Renaturing Buffer [Triton X-100 2.5% (v/v) in ddH₂O] for 30 min at RT. The gel was subsequently incubated in 1× Zymogram Developing Buffer [0.01 M Tris–HCL pH 7.5 (Sigma; 10812846001), 1.25% (v/v) Triton X-100, 5 mM CaCl₂ (Kanto Chemical Co., 07058-00)] for 30 min at RT to allow equilibration. Developing buffer was refreshed and the gel was incubated at 37°C overnight. Finally, the gel was stained with Coomassie Blue R-250 (Thermo; 20278) 0.5% (w/v) for 30 min, and subsequently washed in destaining solution (water:methanol acetic acid at 5:4:1) for 4 times 15 min. The gel was imaged with the Bio-Rad Image Lab system and the image analyzed in Fiji software.

de Vos I.J., Tao E.Y., Ong S.L., Goggi J.L., Scerri T., Wilson G.R., Low C.G., Wong A.S., Grussu D., Stegmann A.P., van Geel M., Janssen R., Amor D.J., Bahlo M., Dunn N.R., Carney T.J., Lockhart P.J., Coull B.J, & van Steensel M.A. (2018). Functional analysis of a hypomorphic allele shows that MMP14 catalytic activity is the prime determinant of the Winchester syndrome phenotype. Human Molecular Genetics, 27(16), 2775-2788.

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Synthesis of Calcium Carbonate Colloids

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A colloidal
suspension of the ACC/PAA precursor was prepared according to our
previously reported method.^{41 (link)} Anhydrous
calcium chloride (CaCl₂) (Wako Pure Chemicals, Osaka, Japan)
was added to an aqueous solution of PAA (M_w = 1.8 × 10³; Aldrich, St Louis, MO, USA) to prepare
a 0.1 M CaCl₂ solution containing 7.2 × 10^–1 wt % PAA. An equal volume of a 0.1 M sodium carbonate (Na₂CO₃) (Kanto Chemical, Tokyo, Japan) solution was added
to the CaCl₂/PAA solution. After reaction for 1 h, the
ACC colloid was collected and washed by centrifugation. All chemical
reagents were used without further purification.

Kuo D., Nishimura T., Kajiyama S, & Kato T. (2018). Bioinspired Environmentally Friendly Amorphous CaCO3-Based Transparent Composites Comprising Cellulose Nanofibers. ACS Omega, 3(10), 12722-12729.

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Emulsified CaCl2 Droplets for Alginate Hydrogel Synthesis

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{CaCl}_{2}

(Kanto Chemical, Tokyo, Japan) aqueous solution was prepared at 2 M, and 80

μ L

of this solution was added into the 1

mL

organic solution of mineral oil (Sigma Aldrich, St. Louis, MO, USA) containing 0.1% (v/v) sorbitan monooleate (Span-80) (Sigma Aldrich, St. Louis, MO, USA) as a surfactant. The

{CaCl}_{2}

aqueous solution and the organic solution were stirred by a vortex mixer for 10 s followed by sonication at 40 kHz for 10 s, resulting in the formation of emulsified

{CaCl}_{2}

aqueous droplets with various sizes, typically ranging from sub-micrometer to tens of micrometer. Sodium alginate (NaAlg) (Sodium Alginate 80–120, FUJIFILM Wako Pure Chemical, Osaka, Japan) aqueous solution was prepared at 3% (w/w) as the dispersed phase. The viscosities of the 2 M

{CaCl}_{2}

aqueous solution, the mineral oil with 0.1% Span-80, and the 3% NaAlg aqueous solution measured with the viscometer (SV-10A, A & D, Tokyo, Japan) were

1.85 \pm 0.03 mPa \cdot s

22.18 \pm 0.24 mPa \cdot s

, and

440.2 \pm 1.9 mPa \cdot s

, respectively. For the experiment of particle image velocimetry (PIV) analysis, 0.3% (v/v) Span-80 was used as a surfactant in the organic phase, and polystyrene beads with the diameter 2

μ

m (Polysciences, Warrington, PA, USA) were added into both the organic and aqueous solutions.

Lee D., Kitahata H, & Ito H. (2021). Fabrication of Microparticles with Front–Back Asymmetric Shapes Using Anisotropic Gelation. Micromachines, 12(9), 1121.

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Investigating NDMA Formation in RO Membranes

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Analytical grade chemicals (NaCl, NaHCO3, CaCl2, NH4Cl, and HCl) were purchased from Kanto Chemical Co. (Tokyo, Japan). A NaOCl solution, with over 12% chlorine concentration, was purchased from Tosoh Co. (Tokyo, Japan), and a stock solution of chloramine was prepared at 0.2 M by mixing NaOCl and NH4Cl solutions. Further, hypobromite stabilized by sulfamic acid was used as stabilized hypobromite in this study. A stock solution of stabilized hypobromite comprised of hypobromite, sulfamic acid and sodium hydroxide was supplied by Organo Co. (Tokyo, Japan).
Chemicals selected as NDMA precursors were DMA, TMA, and N,N-dimethylbenzylamine (DMBA) were purchased from Kanto Chemical Co. (Tokyo, Japan) (Table 1). A flat sheet polyamide composite RO membrane sample (namely ESPA2) were supplied by Hydranautics/Nitto (Oceanside, CA, USA). This study used two types of treated wastewater: an ultrafiltration (UF)-treated wastewater and membrane bioreactor (MBR)-treated wastewater, both of which were collected at wastewater treatment plants in Japan.

Fujioka T., Yoshikawa H., Eguchi M., Boivin S, & Kodamatani H. (2020). Application of stabilized hypobromite for controlling membrane fouling and N-nitrosodimethylamine formation. Chemosphere, 240.

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