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Vevo software

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The Vevo software is a data acquisition and analysis platform designed for use with Fujifilm's Vevo high-resolution ultrasound imaging systems. The software enables the collection and processing of high-quality ultrasound data for research and clinical applications.

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Lab products found in correlation

Vevo 2100, by Fujifilm (2 mentions) Vevo 770 echocardiography system, by Fujifilm (2 mentions) Angiotensin 2, by Merck Group (2 mentions) Rmv 707b transducer, by Fujifilm (1 mentions) Rmv 707b, by Fujifilm (1 mentions) Vevo 770, by Fujifilm (1 mentions) Ms 400, by Fujifilm (1 mentions) Hrv 2, by Kubios (1 mentions) Vevo 3100 ultrasound system, by Fujifilm (1 mentions) Vevo 2100 high resolution imaging system, by Fujifilm (1 mentions) Ivabradine hydrochloride, by Merck Group (1 mentions) Vevo 2100 system, by Fujifilm (1 mentions)

13 protocols using vevo software

1

In vivo Assessment of Outflow Tract Hemodynamics

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OFT hemodynamics and wall dimensions were evaluated in vivo at HH24. To this end, we imaged the heart using ultrasound biomicroscopy (Vevo 2100 with a 40 MHz transducer; Visualsonics, Toronto, ON, Canada) 24 h after banding (n = 10) or sham procedure (n = 8), at approximately HH24. During ultrasound data acquisition, temperature was monitored and controlled with a heat lamp throughout the imaging session. To ensure good contact between the transducer and the embryonic heart, previously warmed (38 °C) Ringer’s solution was placed on top of the egg window. Data were recorded from embryos with heart rates within previously published norms (range: 180–210 beats per minute); as it was previously found that banding does not affect heart rate [30 (link),33 (link)]. OFT external diameters at maximal expansion were measured from B-mode images at the band site, or at an equivalent location in sham controls, using digital calipers and averaging over three cardiac cycles (Vevo software, Visualsonics). Doppler waveforms from within the OFT lumen and at the band region were also obtained, and peak velocity (maximum velocity over the cardiac cycle) was determined from an average of three velocity cycles. Embryos used for this study were discarded after ultrasound data acquisition.
Rennie M.Y., Stovall S., Carson J.P., Danilchik M., Thornburg K.L, & Rugonyi S. (2017). Hemodynamics Modify Collagen Deposition in the Early Embryonic Chicken Heart Outflow Tract. Journal of Cardiovascular Development and Disease, 4(4), 24.
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2

Echocardiographic Assessment of Diastolic Function

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Mice were anesthetized and exmined by echocardiography using a high-resolution Vevo 770 echocardiography system (VisualSonics) with a RMV-707B transducer running at 30 MHz. High-resolution images were obtained for offline measurements with Vevo Software (VisualSonics). For the assessment of diastolic function, an apical four-chamber view was acquired by positioning the transducer as parallel to the mitral inflow as possible. Tissue motion velocity was assessed by spectral pulsed-wave Tissue Doppler imaging, obtained from the mitral septal annulus in the parasternal short axis view. LV diastolic function was assessed by the measurement of the LV transmitral early peak flow velocity (E) to LV transmitral late peak flow velocity (A) wave ratio and mitral annulus early diastole tissue motion (E′) to mitral annulus late diastole tissue motion (A′) wave ratio.
Scotcher J., Prysyazhna O., Boguslavskyi A., Kistamas K., Hadgraft N., Martin E.D., Worthington J., Rudyk O., Rodriguez Cutillas P., Cuello F., Shattock M.J., Marber M.S., Conte M.R., Greenstein A., Greensmith D.J., Venetucci L., Timms J.F, & Eaton P. (2016). Disulfide-activated protein kinase G Iα regulates cardiac diastolic relaxation and fine-tunes the Frank–Starling response. Nature Communications, 7, 13187.
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3

Ultrasound Evaluation of Cardiac Function in Mice

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The mice were anesthetized with isoflurane and fixed on the thermostatic plate of the super high-frequency system. An ultrasonic diagnostic instrument for small animals was used with a central frequency of 30 MHz for ultrasonic examination. The EF and FS of mice were measured by Vevo software developed by VisualSonics in Toronto, Canada.
Shen D, & He Z. (2021). Mesenchymal stem cell-derived exosomes regulate the polarization and inflammatory response of macrophages via miR-21-5p to promote repair after myocardial reperfusion injury. Annals of Translational Medicine, 9(16), 1323.
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4

Comprehensive Echocardiography Imaging Protocol for Mice

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The Vevo MS400 30-MHz linear array transducer had an axial resolution of 50 μm and lateral resolution of 110 μm. Mean frame duration was 3.8 ms, and mean frames per cardiac cycle was 35.1±7.4. Respiration and ECG tracings were synchronised with imaging, and examinations lasted 30 min. Vevo software (VisualSonics, Toronto, Canada) was used for LV M-mode, 2D measurements, and pulsed Doppler velocities; Medimatic software (Medimatic, Genova, Italy) was used for the LA 2D measurements.
Based on a previous pilot study, mice were imaged in: 1) the left parasternal long axis view (2D LV long axis); 2) the parasternal short axis view (M-mode and 2D LV dimensions and systolic function); 3) the standard apical 4-chamber view optimized for the LA cavity (2D LA dimensions and function; mitral valve (MV) annulus dimensions and mitral pulsed Doppler flow velocity) (Fig 1A and 1C); and 4) modified apical 4-chamber views: a) a counter-clockwise rotation to optimize LAA imaging (2D dimensions and pulsed Doppler flow velocity), the LA-LAA duct, and a right pulmonary vein (PV) (Fig 1B and 1D); and b) clock- and counter-clockwise rotations in order to image additional PVs (Fig 2A–2F). Pulsed Doppler PV flow sampling was limited to the former view.
Colazzo F., Castiglioni L., Sironi L., Fontana L., Nobili E., Franzosi M, & Guerrini U. (2015). Murine Left Atrium and Left Atrial Appendage Structure and Function: Echocardiographic and Morphologic Evaluation. PLoS ONE, 10(4), e0125541.
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5

Echocardiographic Evaluation of Mice

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Telemetered (high fat feeding study) or non-telemetered (DAHP study) mice were anesthetised with 2% isoflurane in 0.5 l of oxygen per minute and examined by echocardiography using a non-invasive high resolution Vevo 770 echocardiography system (RMV707B, VisualSonics, Toronto, ON, Canada) with a RMV-707B transducer running at 30 MHz. The core body temperature was maintained at 37 °C with a feedback-regulated body temperature probe. High resolution, two-dimensional B-mode and M-mode images were obtained at the level of the papillary muscles and further analysed offline with Vevo Software (VisualSonics) as before [11] (link).
Rudyk O, & Eaton P. (2017). Examining a role for PKG Iα oxidation in the pathogenesis of cardiovascular dysfunction during diet-induced obesity. Free Radical Biology & Medicine, 110, 390-398.
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6

Cardiac Function Evaluation by Echocardiography

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Echocardiography was performed as described previously [21 ,31 ]. All measurements were performed under Isoflurane (1–2%) anesthesia with continuous monitoring of body temperature, blood pressure and heart rate. Left ventricular end-diastolic diameter (LVEDD) and end-systolic diameter (LVESD), septal wall diastolic thickness (IVSWT) and left ventricular free (posterior) wall diastolic thickness (LVWT) were measured by 2D guided M-mode echocardiography from the parasternal long-axis view by using a 12–38 MHz vascular probe (Vevo 770, 1,000 fps, Visual Sonics, Toronto, Ontario, Canada). Left ventricular end diastolic and systolic volumes (LVEDV and LVESV) and ejection fraction (EF) were calculated using Vevo software (Visual Sonics).
Soler A., Hunter I., Joseph G., Hutcheson R., Hutcheson B., Yang J., Zhang F.F., Joshi S.R., Bradford C., Gotlinger K.H., Maniyar R., Falck J.R., Proctor S., Schwartzman M.L., Gupte S.A, & Rocic P. (2018). Elevated 20-HETE in Metabolic Syndrome Regulates Arterial Stiffness and Systolic Hypertension via MMP12 Activation. Journal of molecular and cellular cardiology, 117, 88-99.
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7

Transverse Aortic Constriction Mouse Model

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C57BL/6NJ male mice (body weight 24 ± 3 g, ~12 weeks old) were anaesthetized with isoflurane placed in the supine position under a dissecting microscope. A 5-mm midline incision was performed above the sternal notch to expose the aorta and carotid arteries. The area at both sides of the aorta was cleared and a 7-0 suture was threaded around the aorta between the carotid arteries and tied against a 27 G needle. The needle was then removed, leaving a narrowing of 0.5 mm in diameter. Animals recovered from anesthesia in a 28°C heated chamber. Control, sham operations were performed by doing the same procedure without tying the knot around the aorta. In some studies, hypertrophy was induced by subcutaneous implantation of osmotic minipumps (model 1002; Alzet) for delivery of angiotensin II (Sigma) at an infusion rate of 1 mg/kg/day (23).
Echocardiography was performed with a Vevo 2100 system at heart rates between 400-450 beats per minute using a 40 MHz linear probe (Visualsonics software version 1.0.0, Canada). Mice were anesthetized with 2% isoflurane and the body temperature was maintained at 37 °C. Ejection fraction, stroke volume, and cardiac output were obtaining from high-resolution motion-mode images at the level of the papillary muscle using Vevo Software (VisualSonics, software version 1.0.0).
Fernandez-Caggiano M., Kamynina A., Francois A.A., Prysyazhna O., Eykyn T.R., Krasemann S., Crespo-Leiro M.G., Vieites M.G., Bianchi K., Morales V., Domenech N, & Eaton P. (2020). Mitochondrial pyruvate carrier abundance mediates pathological cardiac hypertrophy. Nature metabolism, 2(11), 1223-1231.
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8

Echocardiography and ECG in Mice

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Transthoracic echocardiography was performed on healthy mice and before the sacrification (day 7 or day 35). Mice were anesthetized with isoflurane and imaged using a high-frequency, high-resolution imaging system for small animals (Vevo 2100, VisualSonics, Toronto, Canada) equipped with a transducer probe operating at 18–38 MHz (MS-400, VisualSonics). In addition, surface electrocardiography (ECG) signal was acquired during echocardiography. The paws of the mice were attached to the electrode pads of the heated platform (36–37 °C). ECG data were exported from Vevo software (VisualSonics) and analyzed with rodent ECG imaging software (Kubios HRV 2.0, Kuopio, Finland) as previously18 (link).
Vuorio T., Ylä-Herttuala E., Laakkonen J.P., Laidinen S., Liimatainen T, & Ylä-Herttuala S. (2018). Downregulation of VEGFR3 signaling alters cardiac lymphatic vessel organization and leads to a higher mortality after acute myocardial infarction. Scientific Reports, 8, 16709.
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9

Echocardiographic Assessment of Cardiac Function

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In vivo echocardiography was obtained using a Vevo-3100 ultrasound system (Visual Sonics) as previously described (6 (link)). M-mode images in left ventricle short axis view at the proximal level of papillary muscles were used for measurement of left ventricle internal diameter, anterior and posterior wall thickness, left ventricle mass, and relative wall thickness. Parasternal left ventricle long axis view of 2-dimensional image was used for measurement of volume related parameters by tracing the left ventricle wall movement, followed by automatic calculation by the Vevo Software (Visual Sonics). Analysis of 3–5 cardiac cycles was used to generate the average result for each parameter. Doppler echocardiography in an apical 4-chamber view was obtained for diastolic functional analysis. Transmitral flow velocity (E, A) was obtained by pulse-wave Doppler, placing the sample volume at the mitral leaflet tips. Wall velocity (e’) was obtained by tissue Doppler, placing the sample volume at the septal mitral annulus.
Li J., Richmond B., Cluntun A.A., Bia R., Walsh M.A., Shaw K., Symons J.D., Franklin S., Rutter J., Funai K., Shaw R.M, & Hong T. (2023). Cardiac gene therapy treats diabetic cardiomyopathy and lowers blood glucose. JCI Insight, 8(18), e166713.
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10

Transthoracic Echocardiography in Mice

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At the end of the experiment, transthoracic echocardiography was measured using the Vevo2100 High‐Resolution Imaging System (Visual Sonics, Toronto, ON, Canada). Briefly, mice were anaesthetised with 2% isoflurane and maintained with 1.5% isoflurane. Afterword, they were fixed on the console. The 30‐MHz probe was slowly fine‐tuned in B‐mode to obtain clear cardiac images to the maximum extent possible; 2D left ventricular images were then obtained from M‐mode echocardiograms as described previously.
38 (link) Left ventricular ejection fraction (LVEF), left ventricular fractional shortening (LVFS), left ventricular mass (LVM), left ventricular diastolic volume (LV Vol;d) and left ventricular systolic volume (LV Vol;s) were calculated by using the Vevo software (Visual Sonics; version: 3.1.0.13029). At least three cardiac cycles were measured for each mouse, and the average value was calculated.
Cheng Y., Yan M., He S., Xie Y., Wei L., Xuan B., Shang Z., Wu M., Zheng H., Chen Y., Yuan M., Peng J, & Shen A. (2024). Baicalin alleviates angiotensin II‐induced cardiomyocyte apoptosis and autophagy and modulates the AMPK/mTOR pathway. Journal of Cellular and Molecular Medicine, 28(9), e18321.
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