Commercially available ELISA kits were used to measure the concentrations or activity of IFNβ (DIFNB0 or 42400–1, R&D Systems), F3 (DCF300, R&D Systems; ab214091, Abcam), fibrin (MBS265263 or MBS706338, MyBioSource), D-dimer (ab196269, Abcam; MBS723281, MyBioSource), TNF (DTA00D, R&D Systems), IL1A (DLA50, R&D Systems), IL1B (DLB50, R&D Systems), IL6 (D6050, R&D Systems), and HMGB1 (ST51011, IBL International) in the indicated samples. Measurement of GPT/ALT and BUN in the plasma was performed using an IDEXX Catalyst Dx Chemistry Analyzer. PT, APTT, and fibrinogen were measured in an automated coagulometer (Sysmex CA-7000). Platelet count was measured using an IDEXX ProCyte Dx Hematology Analyzer.
Ab196269
Manufactured by Abcam
Sourced in United States
Ab196269 is a recombinant monoclonal antibody targeting an undisclosed protein. It is produced in mammalian cells and purified by affinity chromatography. The product is supplied as a liquid in phosphate buffered saline.
Automatically generated - may contain errors
Lab products found in correlation
Ab214091, by Abcam (1 mentions)
Difnb0, by R&D Systems (1 mentions)
Dcf300, by R&D Systems (1 mentions)
Mbs723281, by MyBioSource (1 mentions)
Dta00d, by R&D Systems (1 mentions)
Dla50, by R&D Systems (1 mentions)
Dlb50, by R&D Systems (1 mentions)
Catalyst dx chemistry analyzer, by IDEXX (1 mentions)
D6050, by R&D Systems (1 mentions)
Procyte dx hematology analyzer, by IDEXX (1 mentions)
Ca 7000, by Sysmex (1 mentions)
2 protocols using ab196269
1
Biomarker Quantification in Plasma
2
Blood Coagulation and D-Dimer Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Two mL blood was taken from the previously collected blood specimen using a pipette. The blood was centrifuged (2,000×g, 10 min), and the supernatant was taken. The blood coagulation analyzer (Dexer X980, German) was used to measure the coagulation parameters (PT, TT, APTT, FIB).
The D-D and AT-III were detected as follows: the resting 3 mL blood of each specimen was centrifuged (3,000 ×g, 30 min), and the supernatant was taken. The D-dimer level was determined by ELISA (Abcam ab196269, USA). The AT-III levels were determined by the chromogenic substrate method (Abcam EP5372, USA). All the procedures were carried out in comply with the product instructions.
The D-D and AT-III were detected as follows: the resting 3 mL blood of each specimen was centrifuged (3,000 ×g, 30 min), and the supernatant was taken. The D-dimer level was determined by ELISA (Abcam ab196269, USA). The AT-III levels were determined by the chromogenic substrate method (Abcam EP5372, USA). All the procedures were carried out in comply with the product instructions.
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