Panc 1

Human PDAC cell lines PANC-1, AsPC-1, Capan-2 and SW1990 were purchased from the Cell Resource Center, Shanghai Institute of Biochemistry and Cell Biology at the Chinese Academy of Sciences and the normal control pancreatic ductal epithelial cell line hTERT-HPNE was obtained from American Type Culture Collection (ATCC). All cell lines were cultured as recommended in growth medium supplemented with 10% fetal bovine serum (FBS) and incubated at 37 °C with a humidified atmosphere of 5 % CO2.
Three small interfering RNAs (siRNAs) against human FAM83B were transfected into human PDAC cells using the DharmaFECT 1 siRNA transfection reagent (Thermo Scientific Dharmacon lnc. USA) according to the manufacturer's instruction, while nonspecific siRNA was applied as a negative control. Real-time PCR was performed to measure the knockdown efficiency of the three FAM83B-siRNAs, and the most efficient one was chosen for further experiment. All the experiments were conducted in the log phase of cell growth. All the siRNAs were designed and synthesized at Genepharm Technologies (Shanghai, China). The sequences targeting FAM83B were listed as follows:
5'- CUCGAGGAGUAUCUGUUUATT-3';
5'- GCCAUCUGAUAGUCUCAGUTT-3';
5'- GGGUCUCAGAAGUUAAGGUTT-3'.

The human PC cell line PANC-1 and the human normal ductal epithelial cell line HPDE6-C7 were purchased from the Cell Resource Center of Shanghai Institute of Biochemistry and Cell Biology, The Chinese Academy of Sciences. The cells were cultured in RPMI-1640 medium (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% FBS (Gibco; Thermo Fisher Scientific, Inc.) and maintained at 37°C with 5% CO₂.

The human PC cell lines BxPC-3, SW1990, and PANC-1 were acquired from Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (Beijing, China). The human PC cell line CFPAC-1 was purchased from Kunming Cell Bank, Chinese Academy of Sciences (Kunming, China). The human normal pancreatic cell line H6C7 was purchased from Shanghai Yaji Biological. BxPC-3 cells were cultured in RPMI 1640 medium (Gibco, USA). H6C7, CFPAC-1, and PANC-1 cells were cultured in DMEM medium (Gibco, USA). SW1990 cells were cultured in L-15 Leibovitz Medium. All cell mediums were supplemented with 10% fetal bovine serum (FBS) (Gibco, USA) at 37 ℃ in a humidified atmosphere containing 5% CO₂.
Vectors encoding STK31 (oe-STK31), miRNA inhibitor (microRNA-543 inhibitor), negative control (NC vector), control mimic, miRNA mimic (microRNA-543 mimic), control inhibitor, LINC00847 overexpression (oe-LINC00847), and the overexpression control (vector-NC) lentiviruses for LINC00847 silencing (si-LINC00847) and the silencing control (si-NC) were purchased from Ribobio (Guangzhou, China). The manufacturer’s procedure was followed for transfection and infection of cells.